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Q2G160

- NANA_STAA8

UniProt

Q2G160 - NANA_STAA8

Protein

N-acetylneuraminate lyase

Gene

nanA

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate.UniRule annotation

    Catalytic activityi

    N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei137 – 1371Involved in proton transfer during cleavageUniRule annotation
    Active sitei165 – 1651Schiff-base intermediate with substrateUniRule annotation

    GO - Molecular functioni

    1. N-acetylneuraminate lyase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-HAMAP
    2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciSAUR93061:GIWJ-279-MONOMER.
    UniPathwayiUPA00629; UER00680.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylneuraminate lyaseUniRule annotation (EC:4.1.3.3UniRule annotation)
    Alternative name(s):
    N-acetylneuraminate pyruvate-lyaseUniRule annotation
    N-acetylneuraminic acid aldolaseUniRule annotation
    Sialate lyaseUniRule annotation
    Sialic acid aldolaseUniRule annotation
    Sialic acid lyaseUniRule annotation
    Gene namesi
    Name:nanAUniRule annotation
    Ordered Locus Names:SAOUHSC_00295
    OrganismiStaphylococcus aureus (strain NCTC 8325)
    Taxonomic identifieri93061 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000008816: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 293293N-acetylneuraminate lyasePRO_1000066938Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi93061.SAOUHSC_00295.

    Structurei

    Secondary structure

    1
    293
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 115
    Helixi24 – 3613
    Beta strandi41 – 477
    Helixi48 – 503
    Helixi52 – 543
    Helixi57 – 6913
    Beta strandi75 – 806
    Helixi86 – 9914
    Beta strandi102 – 1076
    Helixi116 – 13015
    Beta strandi134 – 1385
    Helixi140 – 1434
    Helixi149 – 1568
    Beta strandi161 – 1666
    Helixi171 – 18010
    Beta strandi184 – 1885
    Helixi191 – 1933
    Helixi194 – 1996
    Beta strandi203 – 2086
    Helixi210 – 22516
    Helixi229 – 24921
    Helixi251 – 26010
    Turni261 – 2633
    Helixi278 – 2803
    Helixi281 – 29111

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AH7X-ray2.30A/B/C/D2-293[»]
    4AHOX-ray2.00A/B/C/D1-293[»]
    4AHPX-ray2.10A/B/C/D2-293[»]
    4AHQX-ray1.95A/B/C/D2-293[»]
    4AMAX-ray2.35A/B/C/D2-293[»]
    ProteinModelPortaliQ2G160.
    SMRiQ2G160. Positions 2-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 492Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the DapA family. NanA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0329.
    HOGENOMiHOG000173608.
    KOiK01639.
    OMAiELVPSDM.
    OrthoDBiEOG6W7235.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01237. N_acetylneuram_lyase.
    InterProiIPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    IPR005264. NanA.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.
    PROSITEiPS00666. DHDPS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2G160-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKDLKGLYA ALLVPFDENG QVNEQGLKQI AQNAIETEEL DGLYVNGSSG    50
    ENFLLNTEQK KQVFKVAKEA VGDKVKLIAQ VGSLDLNEAI ELGKYATELG 100
    YDALSAVTPF YYPFTFEEIR DYYFDIIEAT QNNMIIYAIP DLTGVNISIE 150
    QFSELFNHEK IVGVKYTAPN FFLLERIRKA FPDKLILSGF DEMLVQATIS 200
    GVDGAIGSTY NVNGRRARKI FDLARQGQIQ EAYQLQHDSN DIIETVLSMG 250
    IYPTLKEILR HRGIDAGLPK RPFKPFNEAH RQTLDQLIAK YDL 293
    Length:293
    Mass (Da):33,043
    Last modified:March 21, 2006 - v1
    Checksum:i62D7DEFFDC1AF431
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000253 Genomic DNA. Translation: ABD29464.1.
    RefSeqiYP_498885.1. NC_007795.1.

    Genome annotation databases

    EnsemblBacteriaiABD29464; ABD29464; SAOUHSC_00295.
    GeneIDi3918976.
    KEGGisao:SAOUHSC_00295.
    PATRICi19578184. VBIStaAur99865_0268.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000253 Genomic DNA. Translation: ABD29464.1 .
    RefSeqi YP_498885.1. NC_007795.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AH7 X-ray 2.30 A/B/C/D 2-293 [» ]
    4AHO X-ray 2.00 A/B/C/D 1-293 [» ]
    4AHP X-ray 2.10 A/B/C/D 2-293 [» ]
    4AHQ X-ray 1.95 A/B/C/D 2-293 [» ]
    4AMA X-ray 2.35 A/B/C/D 2-293 [» ]
    ProteinModelPortali Q2G160.
    SMRi Q2G160. Positions 2-293.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 93061.SAOUHSC_00295.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD29464 ; ABD29464 ; SAOUHSC_00295 .
    GeneIDi 3918976.
    KEGGi sao:SAOUHSC_00295.
    PATRICi 19578184. VBIStaAur99865_0268.

    Phylogenomic databases

    eggNOGi COG0329.
    HOGENOMi HOG000173608.
    KOi K01639.
    OMAi ELVPSDM.
    OrthoDBi EOG6W7235.

    Enzyme and pathway databases

    UniPathwayi UPA00629 ; UER00680 .
    BioCyci SAUR93061:GIWJ-279-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01237. N_acetylneuram_lyase.
    InterProi IPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    IPR005264. NanA.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    PROSITEi PS00666. DHDPS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Staphylococcus aureus NCTC 8325 genome."
      Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
      (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 8325.

    Entry informationi

    Entry nameiNANA_STAA8
    AccessioniPrimary (citable) accession number: Q2G160
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3