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Q2G160

- NANA_STAA8

UniProt

Q2G160 - NANA_STAA8

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Protein
N-acetylneuraminate lyase
Gene
nanA, SAOUHSC_00295
Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate By similarity.UniRule annotation

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei137 – 1371Involved in proton transfer during cleavage By similarity
Active sitei165 – 1651Schiff-base intermediate with substrate By similarity

GO - Molecular functioni

  1. N-acetylneuraminate lyase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway
  2. carbohydrate metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-279-MONOMER.
UniPathwayiUPA00629; UER00680.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:nanA
Ordered Locus Names:SAOUHSC_00295
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000008816: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293N-acetylneuraminate lyaseUniRule annotation
PRO_1000066938Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00295.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115
Helixi24 – 3613
Beta strandi41 – 477
Helixi48 – 503
Helixi52 – 543
Helixi57 – 6913
Beta strandi75 – 806
Helixi86 – 9914
Beta strandi102 – 1076
Helixi116 – 13015
Beta strandi134 – 1385
Helixi140 – 1434
Helixi149 – 1568
Beta strandi161 – 1666
Helixi171 – 18010
Beta strandi184 – 1885
Helixi191 – 1933
Helixi194 – 1996
Beta strandi203 – 2086
Helixi210 – 22516
Helixi229 – 24921
Helixi251 – 26010
Turni261 – 2633
Helixi278 – 2803
Helixi281 – 29111

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AH7X-ray2.30A/B/C/D2-293[»]
4AHOX-ray2.00A/B/C/D1-293[»]
4AHPX-ray2.10A/B/C/D2-293[»]
4AHQX-ray1.95A/B/C/D2-293[»]
4AMAX-ray2.35A/B/C/D2-293[»]
ProteinModelPortaliQ2G160.
SMRiQ2G160. Positions 2-293.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 492Substrate binding By similarity

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000173608.
KOiK01639.
OMAiELVPSDM.
OrthoDBiEOG6W7235.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01237. N_acetylneuram_lyase.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR005264. NanA.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
PROSITEiPS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2G160-1 [UniParc]FASTAAdd to Basket

« Hide

MNKDLKGLYA ALLVPFDENG QVNEQGLKQI AQNAIETEEL DGLYVNGSSG    50
ENFLLNTEQK KQVFKVAKEA VGDKVKLIAQ VGSLDLNEAI ELGKYATELG 100
YDALSAVTPF YYPFTFEEIR DYYFDIIEAT QNNMIIYAIP DLTGVNISIE 150
QFSELFNHEK IVGVKYTAPN FFLLERIRKA FPDKLILSGF DEMLVQATIS 200
GVDGAIGSTY NVNGRRARKI FDLARQGQIQ EAYQLQHDSN DIIETVLSMG 250
IYPTLKEILR HRGIDAGLPK RPFKPFNEAH RQTLDQLIAK YDL 293
Length:293
Mass (Da):33,043
Last modified:March 21, 2006 - v1
Checksum:i62D7DEFFDC1AF431
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000253 Genomic DNA. Translation: ABD29464.1.
RefSeqiYP_498885.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29464; ABD29464; SAOUHSC_00295.
GeneIDi3918976.
KEGGisao:SAOUHSC_00295.
PATRICi19578184. VBIStaAur99865_0268.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000253 Genomic DNA. Translation: ABD29464.1 .
RefSeqi YP_498885.1. NC_007795.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AH7 X-ray 2.30 A/B/C/D 2-293 [» ]
4AHO X-ray 2.00 A/B/C/D 1-293 [» ]
4AHP X-ray 2.10 A/B/C/D 2-293 [» ]
4AHQ X-ray 1.95 A/B/C/D 2-293 [» ]
4AMA X-ray 2.35 A/B/C/D 2-293 [» ]
ProteinModelPortali Q2G160.
SMRi Q2G160. Positions 2-293.
ModBasei Search...

Protein-protein interaction databases

STRINGi 93061.SAOUHSC_00295.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD29464 ; ABD29464 ; SAOUHSC_00295 .
GeneIDi 3918976.
KEGGi sao:SAOUHSC_00295.
PATRICi 19578184. VBIStaAur99865_0268.

Phylogenomic databases

eggNOGi COG0329.
HOGENOMi HOG000173608.
KOi K01639.
OMAi ELVPSDM.
OrthoDBi EOG6W7235.

Enzyme and pathway databases

UniPathwayi UPA00629 ; UER00680 .
BioCyci SAUR93061:GIWJ-279-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01237. N_acetylneuram_lyase.
InterProi IPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR005264. NanA.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
PROSITEi PS00666. DHDPS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Staphylococcus aureus NCTC 8325 genome."
    Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
    (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 8325.

Entry informationi

Entry nameiNANA_STAA8
AccessioniPrimary (citable) accession number: Q2G160
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 21, 2006
Last modified: July 9, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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