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Q2G0U9

- SLE1_STAA8

UniProt

Q2G0U9 - SLE1_STAA8

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Protein
N-acetylmuramoyl-L-alanine amidase sle1
Gene
sle1, aaa, SAOUHSC_00427
Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Peptidoglycan hydrolase involved in the splitting of the septum during cell division. Binds to both alpha and beta-chains of human fibrinogen as well as fibronectin, which suggests a role in the colonization of host factor-coated material or host tissue. Also exhibits lytic activity against S.carnosus and S.aureus cells but not against M.luteus cells.1 Publication

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

GO - Biological processi

  1. barrier septum assembly Source: UniProtKB-KW
  2. cytolysis Source: UniProtKB-KW
  3. defense response to bacterium Source: UniProtKB-KW
  4. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Cell wall biogenesis/degradation, Septation, Virulence

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-405-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase sle1 (EC:3.5.1.28)
Gene namesi
Name:sle1
Synonyms:aaa
Ordered Locus Names:SAOUHSC_00427
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000008816: Chromosome

Subcellular locationi

Secreted By similarity. Cell surface 1 Publication

GO - Cellular componenti

  1. cell surface Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 334309N-acetylmuramoyl-L-alanine amidase sle1
PRO_0000249326Add
BLAST

Expressioni

Inductioni

Repressed by mgrA. More protein is secreted in a secG mutant (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00427.

Structurei

3D structure databases

ProteinModelPortaliQ2G0U9.
SMRiQ2G0U9. Positions 228-334.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati29 – 7143LysM 1
Add
BLAST
Repeati93 – 13543LysM 2
Add
BLAST
Repeati160 – 20243LysM 3
Add
BLAST
Domaini210 – 334125Peptidase C51
Add
BLAST

Sequence similaritiesi

Contains 3 LysM repeats.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG3942.
HOGENOMiHOG000279823.
KOiK01238.
OMAiEMNYSAS.
OrthoDBiEOG6GTZPF.

Family and domain databases

Gene3Di3.10.350.10. 3 hits.
InterProiIPR007921. CHAP_dom.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF05257. CHAP. 1 hit.
PF01476. LysM. 3 hits.
[Graphical view]
SMARTiSM00257. LysM. 3 hits.
[Graphical view]
SUPFAMiSSF54106. SSF54106. 3 hits.
PROSITEiPS50911. CHAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2G0U9-1 [UniParc]FASTAAdd to Basket

« Hide

MQKKVIAAII GTSAISAVAA TQANAATTHT VKPGESVWAI SNKYGISIAK    50
LKSLNNLTSN LIFPNQVLKV SGSSNSTSNS SRPSTNSGGG SYYTVQAGDS 100
LSLIASKYGT TYQNIMRLNG LNNFFIYPGQ KLKVSGTASS SNAASNSSRP 150
STNSGGGSYY TVQAGDSLSL IASKYGTTYQ KIMSLNGLNN FFIYPGQKLK 200
VTGNASTNSG SATTTNRGYN TPVFSHQNLY TWGQCTYHVF NRRAEIGKGI 250
STYWWNANNW DNAAAADGYT IDNRPTVGSI AQTDVGYYGH VMFVERVNND 300
GSILVSEMNY SAAPGILTYR TVPAYQVNNY RYIH 334
Length:334
Mass (Da):35,836
Last modified:March 21, 2006 - v1
Checksum:i4C1E30AD9DE61D36
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB113206 Genomic DNA. Translation: BAE47503.1.
CP000253 Genomic DNA. Translation: ABD29587.1.
RefSeqiYP_499011.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29587; ABD29587; SAOUHSC_00427.
GeneIDi3919100.
KEGGisao:SAOUHSC_00427.
PATRICi19578430. VBIStaAur99865_0391.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB113206 Genomic DNA. Translation: BAE47503.1 .
CP000253 Genomic DNA. Translation: ABD29587.1 .
RefSeqi YP_499011.1. NC_007795.1.

3D structure databases

ProteinModelPortali Q2G0U9.
SMRi Q2G0U9. Positions 228-334.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 93061.SAOUHSC_00427.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD29587 ; ABD29587 ; SAOUHSC_00427 .
GeneIDi 3919100.
KEGGi sao:SAOUHSC_00427.
PATRICi 19578430. VBIStaAur99865_0391.

Phylogenomic databases

eggNOGi COG3942.
HOGENOMi HOG000279823.
KOi K01238.
OMAi EMNYSAS.
OrthoDBi EOG6GTZPF.

Enzyme and pathway databases

BioCyci SAUR93061:GIWJ-405-MONOMER.

Family and domain databases

Gene3Di 3.10.350.10. 3 hits.
InterProi IPR007921. CHAP_dom.
IPR018392. LysM_dom.
[Graphical view ]
Pfami PF05257. CHAP. 1 hit.
PF01476. LysM. 3 hits.
[Graphical view ]
SMARTi SM00257. LysM. 3 hits.
[Graphical view ]
SUPFAMi SSF54106. SSF54106. 3 hits.
PROSITEi PS50911. CHAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and molecular characterization of an N-acetylmuramyl-L-alanine amidase Sle1 involved in cell separation of Staphylococcus aureus."
    Kajimura J., Fujiwara T., Yamada S., Suzawa Y., Nishida T., Oyamada Y., Hayashi I., Yamagishi J., Komatsuzawa H., Sugai M.
    Mol. Microbiol. 58:1087-1101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-39, FUNCTION.
  2. "The Staphylococcus aureus NCTC 8325 genome."
    Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
    (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 8325.
  3. "Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus."
    Sibbald M.J., Winter T., van der Kooi-Pol M.M., Buist G., Tsompanidou E., Bosma T., Schafer T., Ohlsen K., Hecker M., Antelmann H., Engelmann S., van Dijl J.M.
    J. Bacteriol. 192:3788-3800(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INDUCTION.
    Strain: RN4220.

Entry informationi

Entry nameiSLE1_STAA8
AccessioniPrimary (citable) accession number: Q2G0U9
Secondary accession number(s): Q33E91, Q99WD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 21, 2006
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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