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Protein

Protein-arginine kinase

Gene

mcsB

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific phosphorylation of arginine residues in proteins (By similarity). Is required for stress tolerance and virulence in S.aureus. Acts as a modulator of the repressor activity of CtsR upon heavy metal and oxidative stress caused by these metal ions (PubMed:22902728).UniRule annotation1 Publication

Catalytic activityi

ATP + a [protein]-L-arginine = ADP + a [protein]-N(omega)-phospho-L-arginine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82ATPUniRule annotation1
Binding sitei115ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 28ATPUniRule annotation5
Nucleotide bindingi166 – 170ATPUniRule annotation5
Nucleotide bindingi197 – 202ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

  • stress response to cadmium ion Source: UniProtKB
  • stress response to copper ion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-arginine kinaseUniRule annotation (EC:2.7.14.1UniRule annotation)
Gene namesi
Name:mcsBUniRule annotation
Ordered Locus Names:SAOUHSC_00504
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Hypersensitivity to heavy metal stress, temperature stress, osmotic pressure stress and oxidative stress. Deletion of mcsB also results in sensitivity to variations in pH and lowered expression of the clpC operon under adverse extracellular conditions. Additionally, virulence traits such as hemolytic activity, proteolysis, biofilm formation, and evasion from peritoneal fluid killing are substantially reduced in cells lacking this gene. Interestingly, mutant cells also show a significant reduction in expression of virulence determinants hla and saeS.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000258791 – 335Protein-arginine kinaseAdd BLAST335

Expressioni

Inductioni

Up-regulated by heavy metals such as Cu2+ and Cd2+, but Zn2+ and Co2+ have no effect. Forms part of an operon with ctsR, mcsA and clpC, which is repressed by CtsR.2 Publications

Interactioni

Subunit structurei

Interacts with McsA and CtsR.1 Publication

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00504.

Structurei

3D structure databases

ProteinModelPortaliQ2G0P6.
SMRiQ2G0P6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 244Phosphagen kinase C-terminalUniRule annotationAdd BLAST224

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.UniRule annotation
Contains 1 phosphagen kinase C-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG41066A3. Bacteria.
COG3869. LUCA.
HOGENOMiHOG000082112.
KOiK19405.
OMAiIIMQERV.

Family and domain databases

CDDicd07930. bacterial_phosphagen_kinase. 1 hit.
Gene3Di3.30.590.10. 1 hit.
HAMAPiMF_00602. Prot_Arg_kinase. 1 hit.
InterProiIPR023660. Arg_Kinase.
IPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 2 hits.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
[Graphical view]
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2G0P6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHNIHDNIS QWMKSNEETP IVMSSRIRLA RNLENHVHPL MYATENDGFR
60 70 80 90 100
VINEVQDALP NFELMRLDQM DQQSKMKMVA KHLISPELIK QPAAAVLVND
110 120 130 140 150
DESLSVMINE EDHIRIQAMG TDTTLQALYN QASSIDDELD RSLDISYDEQ
160 170 180 190 200
LGYLTTCPTN IGTGMRASVM LHLPGLSIMK RMTRIAQTIN RFGYTIRGIY
210 220 230 240 250
GEGSQVYGHT YQVSNQLTLG KSELEIIETL TEVVNQIIHE EKQIRQKLDT
260 270 280 290 300
YNQLETQDRV FRSLGILQNC RMITMEEASY RLSEVKLGID LNYIELQNFK
310 320 330
FNELMVAIQS PFLLDEEDDK SVKEKRADIL REHIK
Length:335
Mass (Da):38,610
Last modified:March 21, 2006 - v1
Checksum:iFB1B26912A0A64BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29653.1.
RefSeqiWP_000149503.1. NC_007795.1.
YP_499077.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29653; ABD29653; SAOUHSC_00504.
GeneIDi28379195.
3920416.
KEGGisao:SAOUHSC_00504.
PATRICi19578582. VBIStaAur99865_0451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29653.1.
RefSeqiWP_000149503.1. NC_007795.1.
YP_499077.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ2G0P6.
SMRiQ2G0P6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00504.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29653; ABD29653; SAOUHSC_00504.
GeneIDi28379195.
3920416.
KEGGisao:SAOUHSC_00504.
PATRICi19578582. VBIStaAur99865_0451.

Phylogenomic databases

eggNOGiENOG41066A3. Bacteria.
COG3869. LUCA.
HOGENOMiHOG000082112.
KOiK19405.
OMAiIIMQERV.

Family and domain databases

CDDicd07930. bacterial_phosphagen_kinase. 1 hit.
Gene3Di3.30.590.10. 1 hit.
HAMAPiMF_00602. Prot_Arg_kinase. 1 hit.
InterProiIPR023660. Arg_Kinase.
IPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 2 hits.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
[Graphical view]
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCSB_STAA8
AccessioniPrimary (citable) accession number: Q2G0P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.