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Protein

Sensor histidine kinase GraS

Gene

graS

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system GraR/GraS involved in resistance against cationic antimicrobial peptides (CAMPs). GraS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to GraR. GraR/GraS up-regulates the dlt operon and many other genes, such as other global regulators, adhesins, exoproteins, transporters, toxins, and proteins involved in cell wall synthesis. Down-regulates the expression of many genes involved in RNA and amino acid synthesis or glycolysis. Confers resistance to vancomycin, polymyxin B, lysozyme and LL-37.3 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance, Two-component regulatory system, Virulence

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase GraS (EC:2.7.13.3)
Alternative name(s):
Glycopeptide resistance-associated protein S
Gene namesi
Name:graS
Ordered Locus Names:SAOUHSC_00666
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Transmembranei43 – 63HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003479231 – 346Sensor histidine kinase GraSAdd BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei129Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.Curated

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00666.

Structurei

3D structure databases

ProteinModelPortaliQ2G0D9.
SMRiQ2G0D9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 332Histidine kinasePROSITE-ProRule annotationAdd BLAST207

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EWM. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000032012.
KOiK19077.
OMAiAYLYSAI.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2G0D9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNLKWVAYF LKSRMNWIFW ILFLNFLMLG ISLIDYDFPI DSLFYIVSLN
60 70 80 90 100
LSLTMIFLLL TYFKEVKLYK HFDKDKEIEE IKHKDLAETP FQRHTVDYLY
110 120 130 140 150
RQISAHKEKV VEQQLQLNMH EQTITEFVHD IKTPVTAMKL LIDQEKNQER
160 170 180 190 200
KQALLYEWSR INSMLDTQLY ITRLESQRKD MYFDYVSLKR MVIDEIQLTR
210 220 230 240 250
HISQVKGIGF DVDFKVDDYV YTDIKWCRMI IRQILSNALK YSENFNIEIG
260 270 280 290 300
TELNDQHVSL YIKDYGRGIS KKDMPRIFER GFTSTANRNE TTSSGMGLYL
310 320 330 340
VNSVKDQLGI HLQVTSTVGK GTTVRLIFPL QNEIVERMSE VTNLSF
Length:346
Mass (Da):41,079
Last modified:March 21, 2006 - v1
Checksum:i50820220FB5FDEAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29799.1.
RefSeqiWP_001061252.1. NC_007795.1.
YP_499225.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29799; ABD29799; SAOUHSC_00666.
GeneIDi28379047.
3919429.
KEGGisao:SAOUHSC_00666.
PATRICi19578874. VBIStaAur99865_0597.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29799.1.
RefSeqiWP_001061252.1. NC_007795.1.
YP_499225.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ2G0D9.
SMRiQ2G0D9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00666.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29799; ABD29799; SAOUHSC_00666.
GeneIDi28379047.
3919429.
KEGGisao:SAOUHSC_00666.
PATRICi19578874. VBIStaAur99865_0597.

Phylogenomic databases

eggNOGiENOG4105EWM. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000032012.
KOiK19077.
OMAiAYLYSAI.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRAS_STAA8
AccessioniPrimary (citable) accession number: Q2G0D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.