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Protein

Clumping factor A

Gene

clfA

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface-associated protein implicated in virulence. Promotes bacterial attachment exclusively to the gamma-chain of human fibrinogen. Induces formation of bacterial clumps, which diminish the ability of group IIA phospholipase A2 to cause bacterial phospholipid hydrolysis and killing. Significantly decreases macrophage phagocytosis possibly thanks to the clumps, clumped bacteria being too large to be phagocytosed. Dominant factor responsible for human platelet aggregation, which may be an important mechanism for initiating infective endocarditis. Enhances spleen cell proliferative response in vitro, contributing significantly to the immunostimulatory activity of S.aureus.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Clumping factor A
Alternative name(s):
Fibrinogen receptor A
Fibrinogen-binding protein A
Gene namesi
Name:clfA
Ordered Locus Names:SAOUHSC_00812
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

  • Secretedcell wall PROSITE-ProRule annotation; Peptidoglycan-anchor PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi525N → A: 2-fold reduction in clumping titer compared to wild-type. 1 Publication1
Mutagenesisi526 – 527EV → AS: More than 1000-fold reduction in clumping titer compared to wild-type. No binding to soluble Fg. Dramatic reduction of ability to adhere to immobilized Fg. 2
Mutagenesisi526E → A: 32-fold reduction in clumping titer compared to wild-type. Reduced ability to bind to soluble Fg and to adhere to immobilized Fg. 1 Publication1
Mutagenesisi527V → S: 16- to 32-fold reduction in clumping titer compared to wild-type. activity. Reduced ability to bind to soluble Fg and to adhere to immobilized Fg. 1 Publication1
Mutagenesisi528A → V: 2-fold reduction in clumping titer compared to wild-type; when associated with A-532. 1 Publication1
Mutagenesisi532G → A: 2-fold reduction in clumping titer compared to wild-type; when associated with V-528. 1 Publication1
Mutagenesisi537D → A: 2-fold reduction in clumping titer compared to wild-type. 1 Publication1
Mutagenesisi546E → A: 2-fold reduction in clumping titer compared to wild-type. 1 Publication1
Mutagenesisi559E → A: 2-fold reduction in clumping titer compared to wild-type. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 39Sequence analysisAdd BLAST39
ChainiPRO_000024932440 – 893Clumping factor AAdd BLAST854
PropeptideiPRO_0000249325894 – 927Removed by sortasePROSITE-ProRule annotationAdd BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei893Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation1

Keywords - PTMi

Peptidoglycan-anchor

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00812.

Structurei

Secondary structure

1927
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi233 – 235Combined sources3
Beta strandi237 – 244Combined sources8
Beta strandi247 – 249Combined sources3
Beta strandi257 – 265Combined sources9
Beta strandi274 – 278Combined sources5
Beta strandi283 – 285Combined sources3
Beta strandi305 – 309Combined sources5
Beta strandi315 – 319Combined sources5
Helixi322 – 325Combined sources4
Beta strandi326 – 339Combined sources14
Turni341 – 343Combined sources3
Beta strandi346 – 356Combined sources11
Beta strandi359 – 367Combined sources9
Beta strandi373 – 375Combined sources3
Beta strandi378 – 388Combined sources11
Turni389 – 392Combined sources4
Beta strandi393 – 401Combined sources9
Beta strandi407 – 416Combined sources10
Turni428 – 430Combined sources3
Beta strandi432 – 437Combined sources6
Helixi441 – 443Combined sources3
Helixi459 – 461Combined sources3
Beta strandi462 – 468Combined sources7
Beta strandi471 – 475Combined sources5
Beta strandi481 – 483Combined sources3
Beta strandi487 – 495Combined sources9
Beta strandi505 – 512Combined sources8
Beta strandi518 – 530Combined sources13
Beta strandi533 – 540Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VR3X-ray1.95A/B229-545[»]
ProteinModelPortaliQ2G015.
SMRiQ2G015.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ2G015.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 542Ligand binding A regionAdd BLAST503

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi890 – 894LPXTG sorting signalPROSITE-ProRule annotation5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi68 – 183Thr-richAdd BLAST116
Compositional biasi546 – 565Pro-richAdd BLAST20
Compositional biasi560 – 856Asp/Ser-richAdd BLAST297

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106HV3. Bacteria.
ENOG410Y59N. LUCA.
KOiK14201.
OMAiQTENTSN.

Family and domain databases

Gene3Di2.60.40.1280. 1 hit.
2.60.40.1290. 1 hit.
InterProiIPR011266. Adhesin_Fg-bd_dom_2.
IPR008966. Adhesion_dom.
IPR011252. Fibrogen-bd_dom1.
IPR019948. Gram-positive_anchor.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF00746. Gram_pos_anchor. 1 hit.
PF10425. SdrG_C_C. 1 hit.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SUPFAMiSSF49401. SSF49401. 2 hits.
TIGRFAMsiTIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2G015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMKKKEKHA IRKKSIGVAS VLVGTLIGFG LLSSKEADAS ENSVTQSDSA
60 70 80 90 100
SNESKSNDSS SVSAAPKTDD TNVSDTKTSS NTNNGETSVA QNPAQQETTQ
110 120 130 140 150
SSSTNATTEE TPVTGEATTT TTNQANTPAT TQSSNTNAEE LVNQTSNETT
160 170 180 190 200
SNDTNTVSSV NSPQNSTNAE NVSTTQDTST EATPSNNESA PQSTDASNKD
210 220 230 240 250
VVNQAVNTSA PRMRAFSLAA VAADAPVAGT DITNQLTNVT VGIDSGTTVY
260 270 280 290 300
PHQAGYVKLN YGFSVPNSAV KGDTFKITVP KELNLNGVTS TAKVPPIMAG
310 320 330 340 350
DQVLANGVID SDGNVIYTFT DYVNTKDDVK ATLTMPAYID PENVKKTGNV
360 370 380 390 400
TLATGIGSTT ANKTVLVDYE KYGKFYNLSI KGTIDQIDKT NNTYRQTIYV
410 420 430 440 450
NPSGDNVIAP VLTGNLKPNT DSNALIDQQN TSIKVYKVDN AADLSESYFV
460 470 480 490 500
NPENFEDVTN SVNITFPNPN QYKVEFNTPD DQITTPYIVV VNGHIDPNSK
510 520 530 540 550
GDLALRSTLY GYNSNIIWRS MSWDNEVAFN NGSGSGDGID KPVVPEQPDE
560 570 580 590 600
PGEIEPIPED SDSDPGSDSG SDSNSDSGSD SGSDSTSDSG SDSASDSDSA
610 620 630 640 650
SDSDSASDSD SASDSDSASD SDSDNDSDSD SDSDSDSDSD SDSDSDSDSD
660 670 680 690 700
SDSDSDSDSD SDSDSDSDSD SDSDSDSDSD SDSDSDSDSD SDSDSDSDSD
710 720 730 740 750
SDSDSDSDSD SDSDSDSDSD SDSDSDSDSD SDSDSDSDSD SDSDSDSDSD
760 770 780 790 800
SDSDSDSDSD SDSDSDSASD SDSDSDSDSD SDSDSDSDSD SDSDSDSDSD
810 820 830 840 850
SDSDSDSESD SDSDSDSDSD SDSDSDSDSD SASDSDSGSD SDSSSDSDSE
860 870 880 890 900
SDSNSDSESV SNNNVVPPNS PKNGTNASNK NEAKDSKEPL PDTGSEDEAN
910 920
TSLIWGLLAS IGSLLLFRRK KENKDKK
Length:927
Mass (Da):96,448
Last modified:March 21, 2006 - v1
Checksum:iB429AEB92AEFCABB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29940.1.
RefSeqiWP_001056234.1. NC_007795.1.
YP_499368.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29940; ABD29940; SAOUHSC_00812.
GeneIDi3919375.
KEGGisao:SAOUHSC_00812.
PATRICi19579150. VBIStaAur99865_0734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29940.1.
RefSeqiWP_001056234.1. NC_007795.1.
YP_499368.1. NC_007795.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VR3X-ray1.95A/B229-545[»]
ProteinModelPortaliQ2G015.
SMRiQ2G015.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29940; ABD29940; SAOUHSC_00812.
GeneIDi3919375.
KEGGisao:SAOUHSC_00812.
PATRICi19579150. VBIStaAur99865_0734.

Phylogenomic databases

eggNOGiENOG4106HV3. Bacteria.
ENOG410Y59N. LUCA.
KOiK14201.
OMAiQTENTSN.

Miscellaneous databases

EvolutionaryTraceiQ2G015.

Family and domain databases

Gene3Di2.60.40.1280. 1 hit.
2.60.40.1290. 1 hit.
InterProiIPR011266. Adhesin_Fg-bd_dom_2.
IPR008966. Adhesion_dom.
IPR011252. Fibrogen-bd_dom1.
IPR019948. Gram-positive_anchor.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF00746. Gram_pos_anchor. 1 hit.
PF10425. SdrG_C_C. 1 hit.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SUPFAMiSSF49401. SSF49401. 2 hits.
TIGRFAMsiTIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLFA_STAA8
AccessioniPrimary (citable) accession number: Q2G015
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.