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Protein

3-dehydroquinate dehydratase

Gene

aroD

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.UniRule annotation

Catalytic activityi

3-dehydroquinate = 3-dehydroshikimate + H2O.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 7013-dehydroquinateUniRule annotation
Active sitei133 – 1331Proton donor/acceptorUniRule annotation
Active sitei160 – 1601Schiff-base intermediate with substrateUniRule annotation
Binding sitei202 – 20213-dehydroquinateUniRule annotation
Binding sitei225 – 22513-dehydroquinateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-797-MONOMER.
UniPathwayiUPA00053; UER00086.

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate dehydrataseUniRule annotation (EC:4.2.1.10UniRule annotation)
Short name:
3-dehydroquinaseUniRule annotation
Alternative name(s):
Type I DHQaseUniRule annotation
Type I dehydroquinaseUniRule annotation
Short name:
DHQ1UniRule annotation
Gene namesi
Name:aroDUniRule annotation
Ordered Locus Names:SAOUHSC_00832
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2382383-dehydroquinate dehydratasePRO_1000043187Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00832.

Structurei

3D structure databases

ProteinModelPortaliQ2G002.
SMRiQ2G002. Positions 3-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 3733-dehydroquinate bindingUniRule annotation

Sequence similaritiesi

Belongs to the type-I 3-dehydroquinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105FF2. Bacteria.
COG0710. LUCA.
HOGENOMiHOG000105514.
KOiK03785.
OMAiLFTFRSK.
OrthoDBiEOG6P33BK.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00214. AroD.
InterProiIPR013785. Aldolase_TIM.
IPR001381. DHquinase_I.
[Graphical view]
PfamiPF01487. DHquinase_I. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01093. aroD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2G002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHVEVVATI APQLSIEETL IQKINHRIDA IDVLELRIDQ IENVTVDQVA
60 70 80 90 100
EMITKLKVMQ DSFKLLVTYR TKLQGGYGQF TNDSYLNLIS DLANINGIDM
110 120 130 140 150
IDIEWQADID IEKHQRIITH LQQYNKEVVI SHHNFESTPP LDELQFIFFK
160 170 180 190 200
MQKFNPEYVK LAVMPHNKND VLNLLQAMST FSDTMDCKVV GISMSKLGLI
210 220 230
SRTAQGVFGG ALTYGCIGVP QAPGQIDVTD LKAQVTLY
Length:238
Mass (Da):26,869
Last modified:March 21, 2006 - v1
Checksum:i108F24B67BDD76A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29958.1.
RefSeqiWP_000150017.1. NC_007795.1.
YP_499386.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29958; ABD29958; SAOUHSC_00832.
GeneIDi3918944.
KEGGisao:SAOUHSC_00832.
PATRICi19579186. VBIStaAur99865_0752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD29958.1.
RefSeqiWP_000150017.1. NC_007795.1.
YP_499386.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ2G002.
SMRiQ2G002. Positions 3-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29958; ABD29958; SAOUHSC_00832.
GeneIDi3918944.
KEGGisao:SAOUHSC_00832.
PATRICi19579186. VBIStaAur99865_0752.

Phylogenomic databases

eggNOGiENOG4105FF2. Bacteria.
COG0710. LUCA.
HOGENOMiHOG000105514.
KOiK03785.
OMAiLFTFRSK.
OrthoDBiEOG6P33BK.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00086.
BioCyciSAUR93061:GIWJ-797-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00214. AroD.
InterProiIPR013785. Aldolase_TIM.
IPR001381. DHquinase_I.
[Graphical view]
PfamiPF01487. DHquinase_I. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01093. aroD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Staphylococcus aureus NCTC 8325 genome."
    Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
    (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 8325.

Entry informationi

Entry nameiAROD_STAA8
AccessioniPrimary (citable) accession number: Q2G002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 21, 2006
Last modified: March 16, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.