Reviewed, UniProtKB/Swiss-Prot Q2FZW6 (DLTA_STAA8)

Names and origin

Protein names

Recommended name:
    D-alanine--poly(phosphoribitol) ligase subunit 1
    EC=6.1.1.13
Alternative name(s):
    D-alanine-activating enzyme
      Short name=DAE
    D-alanine-D-alanyl carrier protein ligase
      Short name=DCL

Gene names

Name:	dltA
Ordered Locus Names:	SAOUHSC_00869

Organism

Staphylococcus aureus (strain NCTC 8325) [Complete proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the biosynthesis of D-alanyl-lipoteichoic acid (LTA). Catalyzes an ATP-dependent two-step reaction where it forms a high energy D-alanyl AMP intermediate and transfers the alanyl residues from AMP to Dcp By similarity.
Catalytic activity	ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate). HAMAP MF_00593
Pathway	Cell wall biogenesis; lipoteichoic acid biosynthesis. HAMAP MF_00593
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	teichoic acid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-poly(phosphoribitol) ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	D-alanine--poly(phosphoribitol) ligase subunit 1 HAMAP MF_00593		PRO_0000289546

Sequences

	Sequence		Length	Mass (Da)
	Q2FZW6-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: FFA535607F1FD328	FASTA	485	54,670
	10 20 30 40 50 60 MTDIINKLQA FADANPQSIA VRHTTDELTY QQLMDESSKL AHRLQGSKKP MILFGHMSPY 70 80 90 100 110 120 MIVGMIGAIK AGCGYVPVDT SIPEDRIKMI INKVQPEFVF NTTDESFESL EGEVFTIEDI 130 140 150 160 170 180 KTSQDPVIFD SQIKDNDTVY TIFTSGSTGE PKGVQIEYAS LVQFTEWMLE LNKSGNEQQW 190 200 210 220 230 240 LNQAPFSFDL SVMAIYPCLA SGGTLNLVDK NMINKPKLLN EMLTATPINI WVSTPSFMEM 250 260 270 280 290 300 CLLLPTLNEE QYGSLNEFFF CGEILPHRAA KALVNRFPSA TIYNTYGPTE ATVAVTSIQI 310 320 330 340 350 360 TQEILDQYPT LPVGVERPGA RLSTTDEGEL VIEGQSVSLG YLKNDQKTAE VFNFDDGIRT 370 380 390 400 410 420 YHTGDKAKFE NGQWFIQGRI DFQIKLNGYR MELEEIETQL RQSEFVKEAI VVPVYKNDKV 430 440 450 460 470 480 IHLIGAIVPT TEVTDNAEMT KNIKNDLKSR LPEYMIPRKF EWMEQLPLTS NGKIDRKKIA EVING

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inactivation of the dlt operon in Staphylococcus aureus confers sensitivity to defensins, protegrins, and other antimicrobial peptides." Peschel A., Otto M., Jack R.W., Kalbacher H., Jung G., Goetz F. J. Biol. Chem. 274:8405-8410(1999) [PubMed: 10085071] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF101234 Genomic DNA. Translation: AAD21957.1. CP000253 Genomic DNA. Translation: ABD29994.1.
RefSeq	YP_499422.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3919216.
GenomeReviews	Gene locus SAOUHSC_00869 in contig CP000253_GR.
KEGG	sao:SAOUHSC_00869.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FZW6.
OMA	Q2FZW6. CVKSGHA.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_00869-MON.
Family and domain databases
HAMAP	MF_00593. [Tree]
InterPro	IPR010071. AA_adenyl_domain. IPR000873. AMP-dep_Synth/Lig. IPR010072. D_ala_DACP_lig. [Graphical view]
PANTHER	PTHR11968:SF34. D_ala_DACP_lig. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01733. AA-adenyl-dom. 1 hit. TIGR01734. D-ala-DACP-lig. 1 hit.
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DLTA_STAA8

Accession

Primary (citable) accession number: Q2FZW6
Secondary accession number(s): P68877, Q53661, Q9S673

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)