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Protein

Processive diacylglycerol beta-glucosyltransferase

Gene

ugtP

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Processive glucosyltransferase involved in the biosynthesis of both the bilayer- and non-bilayer-forming membrane glucolipids. Is able to successively transfer two glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of beta-monoglucosyl-DAG (3-O-(beta-D-glucopyranosyl)-1,2-diacyl-sn-glycerol) and beta-diglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). Beta-diglucosyl-DAG is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA). UgtP can only use UDP-glucose as sugar donor.UniRule annotation2 Publications

Catalytic activityi

UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.1 Publication
UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol.UniRule annotation1 Publication

Kineticsi

  1. KM=0.7 mM for UDP-glucose (at 15 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Pathwayi: diglucosyl-diacylglycerol biosynthesis

    This protein is involved in the pathway diglucosyl-diacylglycerol biosynthesis, which is part of Glycolipid metabolism.UniRule annotation1 Publication
    View all proteins of this organism that are known to be involved in the pathway diglucosyl-diacylglycerol biosynthesis and in Glycolipid metabolism.

    GO - Molecular functioni

    • 1,2-diacylglycerol 3-glucosyltransferase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciSAUR93061:GIWJ-917-MONOMER.
    UniPathwayiUPA00894.

    Protein family/group databases

    CAZyiGT28. Glycosyltransferase Family 28.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Processive diacylglycerol beta-glucosyltransferaseUniRule annotation (EC:2.4.1.315)
    Alternative name(s):
    Beta-diglucosyldiacylglycerol synthaseUniRule annotation
    Short name:
    Beta-DGSUniRule annotation
    Short name:
    DGlcDAG synthaseUniRule annotation
    Short name:
    Glc2-DAG synthaseUniRule annotation
    Beta-gentiobiosyldiacylglycerol synthaseUniRule annotation
    Beta-monoglucosyldiacylglycerol synthaseUniRule annotation
    Short name:
    Beta-MGSUniRule annotation
    Short name:
    MGlcDAG synthaseUniRule annotation
    Diglucosyl diacylglycerol synthase (1,6-linking)
    Glucosyl-beta-1,6-glucosyldiacylglycerol synthaseUniRule annotation
    UDP glucosyltransferaseUniRule annotation
    UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferaseUniRule annotation
    Gene namesi
    Name:ugtPUniRule annotation
    Synonyms:ypfP
    Ordered Locus Names:SAOUHSC_00953
    OrganismiStaphylococcus aureus (strain NCTC 8325)
    Taxonomic identifieri93061 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
    Proteomesi
    • UP000008816 Componenti: Chromosome

    Subcellular locationi

    • Cell membrane UniRule annotation1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene are defective in beta-diglucosyl-DAG and are smaller than wild-type. Diacylglycerol (DAG) is the anchor of the LTA in the mutant.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391Processive diacylglycerol beta-glucosyltransferasePRO_0000308444Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi93061.SAOUHSC_00953.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2FZP7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 28 family. UgtP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4108QBV. Bacteria.
    COG0707. LUCA.
    HOGENOMiHOG000221565.
    KOiK03429.
    OMAiEPAGHIV.
    OrthoDBiEOG61VZFD.

    Family and domain databases

    HAMAPiMF_01280. Diacylglyc_glucosyltr.
    InterProiIPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    IPR023589. Pro_diacylglycrl_glcsylTrfase.
    [Graphical view]
    PfamiPF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2FZP7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVTQNKKILI ITGSFGNGHM QVTQSIVNQL NDMNLDHLSV IEHDLFMEAH
    60 70 80 90 100
    PILTSICKKW YINSFKYFRN MYKGFYYSRP DKLDKCFYKY YGLNKLINLL
    110 120 130 140 150
    IKEKPDLILL TFPTPVMSVL TEQFNINIPV ATVMTDYRLH KNWITPYSTR
    160 170 180 190 200
    YYVATKETKQ DFIDVGIDPS TVKVTGIPID NKFETPINQK QWLIDNNLDP
    210 220 230 240 250
    DKQTILMSAG AFGVSKGFDT MITDILAKSA NAQVVMICGK SKELKRSLTA
    260 270 280 290 300
    KFKSNENVLI LGYTKHMNEW MASSQLMITK PGGITITEGF ARCIPMIFLN
    310 320 330 340 350
    PAPGQELENA LYFEEKGFGK IADTPEEAIK IVASLTNGNE QLTNMISTME
    360 370 380 390
    QDKIKYATQT ICRDLLDLIG HSSQPQEIYG KVPLYARFFV K
    Length:391
    Mass (Da):44,548
    Last modified:March 21, 2006 - v1
    Checksum:iA1D6E96C91B7E99D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000253 Genomic DNA. Translation: ABD30078.1.
    RefSeqiWP_000258650.1. NC_007795.1.
    YP_499506.1. NC_007795.1.

    Genome annotation databases

    EnsemblBacteriaiABD30078; ABD30078; SAOUHSC_00953.
    GeneIDi3920664.
    KEGGisao:SAOUHSC_00953.
    PATRICi19579428. VBIStaAur99865_0875.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000253 Genomic DNA. Translation: ABD30078.1.
    RefSeqiWP_000258650.1. NC_007795.1.
    YP_499506.1. NC_007795.1.

    3D structure databases

    ProteinModelPortaliQ2FZP7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi93061.SAOUHSC_00953.

    Protein family/group databases

    CAZyiGT28. Glycosyltransferase Family 28.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABD30078; ABD30078; SAOUHSC_00953.
    GeneIDi3920664.
    KEGGisao:SAOUHSC_00953.
    PATRICi19579428. VBIStaAur99865_0875.

    Phylogenomic databases

    eggNOGiENOG4108QBV. Bacteria.
    COG0707. LUCA.
    HOGENOMiHOG000221565.
    KOiK03429.
    OMAiEPAGHIV.
    OrthoDBiEOG61VZFD.

    Enzyme and pathway databases

    UniPathwayiUPA00894.
    BioCyciSAUR93061:GIWJ-917-MONOMER.

    Family and domain databases

    HAMAPiMF_01280. Diacylglyc_glucosyltr.
    InterProiIPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    IPR023589. Pro_diacylglycrl_glcsylTrfase.
    [Graphical view]
    PfamiPF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The Staphylococcus aureus NCTC 8325 genome."
      Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
      (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 8325.
    2. "Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase."
      Kiriukhin M.Y., Debabov D.V., Shinabarger D.L., Neuhaus F.C.
      J. Bacteriol. 183:3506-3514(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, ROLE IN GLYCOLIPID AND LTA BIOSYNTHESIS, DISRUPTION PHENOTYPE.
      Strain: RN4220.
    3. "Genes required for glycolipid synthesis and lipoteichoic acid anchoring in Staphylococcus aureus."
      Gruendling A., Schneewind O.
      J. Bacteriol. 189:2521-2530(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PATHWAY, ROLE IN GLYCOLIPID AND LTA BIOSYNTHESIS.
      Strain: RN4220.

    Entry informationi

    Entry nameiUGTP_STAA8
    AccessioniPrimary (citable) accession number: Q2FZP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: March 21, 2006
    Last modified: March 16, 2016
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.