Q2FZP7 (UGTP_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Processive diacylglycerol glucosyltransferase
EC=2.4.1.-

Alternative name(s):
Beta-gentiobiosyldiacylglycerol synthase
DGlcDAG synthase
Diglucosyldiacylglycerol synthase
Glc2-DAG synthase
Monoglucosyldiacylglycerol synthase
Short name=MGlcDAG synthase
Triglucosyldiacylglycerol synthase
Short name=TGlcDAG synthase
UDP glucosyltransferase
UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferase

Gene names

Name:	ugtP
Synonyms:	ypfP
Ordered Locus Names:	SAOUHSC_00953

Organism

Staphylococcus aureus (strain NCTC 8325)

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is a processive glucosyltransferase involved in the synthesis of both bilayer- and non-bilayer-forming membrane glycolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of mono-, di- and triglucosyldiacylglycerol. Diglucosyldiacylglycerol (Glc2-DAG) is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA). Ref.2 Ref.3
Catalytic activity	UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol.
Pathway	Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis. Ref.3
Sequence similarities	Belongs to the glycosyltransferase 28 family. UgtP subfamily.
Biophysicochemical properties	Kinetic parameters: KM=0.7 mM for UDP-glucose (at 15 degrees Celsius) Ref.2 pH dependence: Optimum pH is 7.5.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolipid biosynthetic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro glucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Processive diacylglycerol glucosyltransferase HAMAP MF_01280		PRO_0000308444

Sequences

	Sequence		Length	Mass (Da)
	Q2FZP7 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: A1D6E96C91B7E99D	FASTA	391	44,548
	10 20 30 40 50 60 MVTQNKKILI ITGSFGNGHM QVTQSIVNQL NDMNLDHLSV IEHDLFMEAH PILTSICKKW 70 80 90 100 110 120 YINSFKYFRN MYKGFYYSRP DKLDKCFYKY YGLNKLINLL IKEKPDLILL TFPTPVMSVL 130 140 150 160 170 180 TEQFNINIPV ATVMTDYRLH KNWITPYSTR YYVATKETKQ DFIDVGIDPS TVKVTGIPID 190 200 210 220 230 240 NKFETPINQK QWLIDNNLDP DKQTILMSAG AFGVSKGFDT MITDILAKSA NAQVVMICGK 250 260 270 280 290 300 SKELKRSLTA KFKSNENVLI LGYTKHMNEW MASSQLMITK PGGITITEGF ARCIPMIFLN 310 320 330 340 350 360 PAPGQELENA LYFEEKGFGK IADTPEEAIK IVASLTNGNE QLTNMISTME QDKIKYATQT 370 380 390 ICRDLLDLIG HSSQPQEIYG KVPLYARFFV K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.
[2]	"Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase." Kiriukhin M.Y., Debabov D.V., Shinabarger D.L., Neuhaus F.C. J. Bacteriol. 183:3506-3514(2001) [PubMed: 11344159] [Abstract] Cited for: FUNCTION, ROLE IN GLYCOLIPID AND LTA BIOSYNTHESIS, BIOPHYSICOCHEMICAL PROPERTIES.
[3]	"Genes required for glycolipid synthesis and lipoteichoic acid anchoring in Staphylococcus aureus." Gruendling A., Schneewind O. J. Bacteriol. 189:2521-2530(2007) [PubMed: 17209021] [Abstract] Cited for: PATHWAY, ROLE IN GLYCOLIPID AND LTA BIOSYNTHESIS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000253 Genomic DNA. Translation: ABD30078.1.
RefSeq	YP_499506.1. NC_007795.1.
3D structure databases
ProteinModelPortal	Q2FZP7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FZP7.
Protein family/group databases
CAZy	GT28. Glycosyltransferase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000028008; EBSTAP00000026959; EBSTAG00000028007.
GeneID	3920664.
GenomeReviews	Gene locus SAOUHSC_00953 in contig CP000253_GR.
KEGG	sao:SAOUHSC_00953.
PATRIC	19579428. VBIStaAur99865_0875.
Phylogenomic databases
eggNOG	COG0707.
GeneTree	EBGT00050000024210.
HOGENOM	HBG535586.
OMA	EPAGHIV.
ProtClustDB	PRK13608.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_00953-MONOMER.
Family and domain databases
HAMAP	MF_01280. Diacylglyc_glucosyltr. [Tree]
InterPro	IPR009695. Diacylglyc_glucosyltr_N. IPR007235. Glyco_trans_28_C. IPR023589. Pro_diacylglycrl_glcsylTrfase. [Graphical view]
KO	K03429.
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF06925. MGDG_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UGTP_STAA8

Accession

Primary (citable) accession number: Q2FZP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program