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Q2FZE9

- ISDA_STAA8

UniProt

Q2FZE9 - ISDA_STAA8

Protein

Iron-regulated surface determinant protein A

Gene

isdA

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Transfers its hemin to hemin-free IsdC (apo-IsdC) directly probably through the activation of the holo-IsdA-apo-IsdC complex and driven by the higher affinity of apo-IsdC for the cofactor. The reaction is reversible. Binds transferrin, lactoferrin, heme, hemoglobin, hemin, fetuin, asialofetuin and protein A. Also binds fibronectin and chains B-beta and gamma of fibrinogen, promoting clumping of S.aureus with fibrinogen. Was also shown to adhere to plastic. Inactivation of isdA leads to a decrease both in the amount of heme-iron associated with S.aureus cells and the amount of heme-iron that enters the staphylococcal cytoplasm. This suggests that IsdA could play a role in the removal of heme from hemoglobin. It was also demonstrated that the IsdA-mediated iron-acquisition system from transferrin could play only an ancillary role in the iron uptake whereas the siderophore-mediated iron-acquisition system from transferrin seems to play an essential or dominant role. May function as a reservoir for heme. Involved in adherence of S.aureus to human desquamated nasal epithelial cells and is required for nasal colonization. Protects S.aureus against the bactericidal protease activity of apolactoferrin in vitro and confers resistance to bovine lactoferricin. Also IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei75 – 751Heme1 Publication
    Binding sitei82 – 821Heme1 Publication
    Metal bindingi166 – 1661Iron (heme axial ligand)

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciSAUR93061:GIWJ-1040-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Iron-regulated surface determinant protein A
    Alternative name(s):
    Fur-regulated protein A
    Staphylococcal transferrin-binding protein A
    Gene namesi
    Name:isdA
    Synonyms:frpA, stbA
    Ordered Locus Names:SAOUHSC_01081
    OrganismiStaphylococcus aureus (strain NCTC 8325)
    Taxonomic identifieri93061 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000008816: Chromosome

    Subcellular locationi

    Secretedcell wall 1 Publication; Peptidoglycan-anchor 1 Publication

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell wall, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi83 – 831H → A: Does not affect heme-binding. 1 Publication
    Mutagenesisi87 – 871Y → A: Decreases heme-binding. 1 Publication
    Mutagenesisi101 – 1011Y → A: Does not affect heme-binding. 1 Publication
    Mutagenesisi102 – 1021Y → A: Does not affect heme-binding. 1 Publication
    Mutagenesisi150 – 1501Y → A: Does not affect heme-binding. 1 Publication
    Mutagenesisi166 – 1661Y → A: Almost abolishes heme-binding. 1 Publication
    Mutagenesisi170 – 1701Y → A: Strongly decreases heme-binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4646By similarityAdd
    BLAST
    Chaini47 – 316270Iron-regulated surface determinant protein APRO_0000247966Add
    BLAST
    Propeptidei317 – 35034Removed by sortase APROSITE-ProRule annotationPRO_0000247967Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei316 – 3161Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

    Keywords - PTMi

    Peptidoglycan-anchor

    Expressioni

    Inductioni

    Repressed by fur in the presence of iron.

    Interactioni

    Subunit structurei

    Monomer. Interacts with IsdC By similarity.By similarity

    Protein-protein interaction databases

    STRINGi93061.SAOUHSC_01081.

    Structurei

    Secondary structure

    1
    350
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi65 – 695
    Beta strandi71 – 755
    Beta strandi78 – 814
    Helixi83 – 875
    Beta strandi90 – 978
    Beta strandi100 – 10910
    Helixi110 – 1123
    Beta strandi113 – 1197
    Beta strandi128 – 1347
    Turni135 – 1384
    Beta strandi139 – 1468
    Beta strandi152 – 16110
    Helixi162 – 1643
    Beta strandi166 – 17813

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ITEX-ray1.60A/B62-184[»]
    2ITFX-ray1.90A/B/C/D62-184[»]
    ProteinModelPortaliQ2FZE9.
    SMRiQ2FZE9. Positions 63-184.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ2FZE9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini62 – 184123NEATPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi313 – 3175LPXTG sorting signalPROSITE-ProRule annotation

    Domaini

    The NEAT domain is responsible for binding Fe3+ and Fe2+ heme and fibrinogen. The NEAT domain is an inhibitor of apolactoferrin activity, while the C-domain confers resistance to bovine lactoferricin By similarity.By similarity

    Sequence similaritiesi

    Contains 1 NEAT domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5386.
    HOGENOMiHOG000107381.
    KOiK14193.
    OMAiVNDDKKA.
    OrthoDBiEOG68SVXG.

    Family and domain databases

    InterProiIPR019948. Gram-positive_anchor.
    IPR019931. LPXTG_anchor.
    IPR006635. NEAT_dom.
    [Graphical view]
    PfamiPF00746. Gram_pos_anchor. 1 hit.
    PF05031. NEAT. 1 hit.
    [Graphical view]
    SMARTiSM00725. NEAT. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
    PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
    PS50978. NEAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2FZE9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKHYLNSKY QSEQRSSAMK KITMGTASII LGSLVYIGAD SQQVNAATEA    50
    TNATNNQSTQ VSQATSQPIN FQVQKDGSSE KSHMDDYMQH PGKVIKQNNK 100
    YYFQTVLNNA SFWKEYKFYN ANNQELATTV VNDNKKADTR TINVAVEPGY 150
    KSLTTKVHIV VPQINYNHRY TTHLEFEKAI PTLADAAKPN NVKPVQPKPA 200
    QPKTPTEQTK PVQPKVEKVK PTVTTTSKVE DNHSTKVVST DTTKDQTKTQ 250
    TAHTVKTAQT AQEQNKVQTP VKDVATAKSE SNNQAVSDNK SQQTNKVTKH 300
    NETPKQASKA KELPKTGLTS VDNFISTVAF ATLALLGSLS LLLFKRKESK 350
    Length:350
    Mass (Da):38,746
    Last modified:March 21, 2006 - v1
    Checksum:i14882D25C0EA3CA6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti105 – 1051T → A in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti134 – 1341N → D in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti222 – 2221T → A in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti225 – 2262TT → AP in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti229 – 2346VEDNHS → NENRQT in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti240 – 2423TDT → SEA in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti247 – 2482TK → SQ in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti251 – 2533TAH → SAR in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti258 – 2581A → T in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti263 – 2631E → D in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti300 – 3001H → Q in AAL33768. (PubMed:11952908)Curated
    Sequence conflicti303 – 3042TP → VH AA sequence (PubMed:11952908)Curated
    Sequence conflicti307 – 3071A → GPSKD AA sequence (PubMed:11952908)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY061874 Genomic DNA. Translation: AAL33768.1.
    CP000253 Genomic DNA. Translation: ABD30197.1.
    RefSeqiYP_499627.1. NC_007795.1.

    Genome annotation databases

    EnsemblBacteriaiABD30197; ABD30197; SAOUHSC_01081.
    GeneIDi3919243.
    KEGGisao:SAOUHSC_01081.
    PATRICi19579665. VBIStaAur99865_0991.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY061874 Genomic DNA. Translation: AAL33768.1 .
    CP000253 Genomic DNA. Translation: ABD30197.1 .
    RefSeqi YP_499627.1. NC_007795.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ITE X-ray 1.60 A/B 62-184 [» ]
    2ITF X-ray 1.90 A/B/C/D 62-184 [» ]
    ProteinModelPortali Q2FZE9.
    SMRi Q2FZE9. Positions 63-184.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 93061.SAOUHSC_01081.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD30197 ; ABD30197 ; SAOUHSC_01081 .
    GeneIDi 3919243.
    KEGGi sao:SAOUHSC_01081.
    PATRICi 19579665. VBIStaAur99865_0991.

    Phylogenomic databases

    eggNOGi COG5386.
    HOGENOMi HOG000107381.
    KOi K14193.
    OMAi VNDDKKA.
    OrthoDBi EOG68SVXG.

    Enzyme and pathway databases

    BioCyci SAUR93061:GIWJ-1040-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q2FZE9.
    PROi Q2FZE9.

    Family and domain databases

    InterProi IPR019948. Gram-positive_anchor.
    IPR019931. LPXTG_anchor.
    IPR006635. NEAT_dom.
    [Graphical view ]
    Pfami PF00746. Gram_pos_anchor. 1 hit.
    PF05031. NEAT. 1 hit.
    [Graphical view ]
    SMARTi SM00725. NEAT. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
    PROSITEi PS50847. GRAM_POS_ANCHORING. 1 hit.
    PS50978. NEAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transferrin binding in Staphylococcus aureus: involvement of a cell wall-anchored protein."
      Taylor J.M., Heinrichs D.E.
      Mol. Microbiol. 43:1603-1614(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 47-60, INTERACTION WITH TRANSFERRIN, SUBCELLULAR LOCATION.
    2. "The Staphylococcus aureus NCTC 8325 genome."
      Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
      (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 8325.
    3. "Conservation, surface exposure, and in vivo expression of the Frp family of iron-regulated cell wall proteins in Staphylococcus aureus."
      Morrissey J.A., Cockayne A., Hammacott J., Bishop K., Denman-Johnson A., Hill P.J., Williams P.
      Infect. Immun. 70:2399-2407(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 47-56, NON-SPECIFIC BINDING TO TRANSFERRIN AND PROTEIN A, BINDING TO PLASTIC, REGULATION BY FUR.
    4. "IsdA of Staphylococcus aureus is a broad spectrum, iron-regulated adhesin."
      Clarke S.R., Wiltshire M.D., Foster S.J.
      Mol. Microbiol. 51:1509-1519(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HEMIN; FETUIN; HEMOGLOBIN; TRANSFERRIN; FIBRONECTIN AND FIBRINOGEN, IRON-REGULATED EXPRESSION, ROLE OF THE NEAT DOMAIN.
    5. "Characterization of the heme binding properties of Staphylococcus aureus IsdA."
      Vermeiren C.L., Pluym M., Mack J., Heinrichs D.E., Stillman M.J.
      Biochemistry 45:12867-12875(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    6. "Heme binding in the NEAT domains of IsdA and IsdC of Staphylococcus aureus."
      Pluym M., Muryoi N., Heinrichs D.E., Stillman M.J.
      J. Inorg. Biochem. 102:480-488(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE OF THE NEAT DOMAIN.
    7. "Haem recognition by a Staphylococcus aureus NEAT domain."
      Grigg J.C., Vermeiren C.L., Heinrichs D.E., Murphy M.E.P.
      Mol. Microbiol. 63:139-149(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 62-184 OF APOPROTEIN AND IN COMPLEX WITH HEME, ROLE IN IRON ACQUISITION, DOMAIN, MUTAGENESIS OF HIS-83; TYR-87; TYR-101; TYR-102; TYR-150; TYR-166 AND TYR-170.

    Entry informationi

    Entry nameiISDA_STAA8
    AccessioniPrimary (citable) accession number: Q2FZE9
    Secondary accession number(s): Q9KW67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 22, 2006
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Expressed in vivo during infection or colonization by S.aureus.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3