Reviewed, UniProtKB/Swiss-Prot Q2FZE2 (ISDG_STAA8)

Names and origin

Protein names

Recommended name:
    Heme-degrading monooxygenase isdG
    EC=1.14.99.3
Alternative name(s):
    Iron-regulated surface determinant isdG
    Iron-responsive surface determinant isdG
    Heme oxygenase

Gene names

Name:	isdG
Ordered Locus Names:	SAOUHSC_01089

Organism

Staphylococcus aureus (strain NCTC 8325) [Complete proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron. Ref.2
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. Ref.2
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. Ref.2
Induction	Transcriptionally regulated by iron and the ferric uptake repressor (fur) protein. Ref.2
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase isdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: HAMAP heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: EC monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 107	107	Heme-degrading monooxygenase isdG HAMAP MF_01272		PRO_0000270093
Sites
	Metal binding	7	1	Iron Potential
	Metal binding	77	1	Iron (heme axial ligand) Potential
	Site	67	1	Transition state stabilizer Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2FZE2-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: DB13A134D5EC4FF0	FASTA	107	12,546
	10 20 30 40 50 60 MKFMAENRLT LTKGTAKDII ERFYTRHGIE TLEGFDGMFV TQTLEQEDFD EVKILTVWKS 70 80 90 100 KQAFTDWLKS DVFKAAHKHV RSKNEDESSP IINNKVITYD IGYSYMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"IsdG and IsdI, heme-degrading enzymes in the cytoplasm of Staphylococcus aureus." Skaar E.P., Gaspar A.H., Schneewind O. J. Biol. Chem. 279:436-443(2004) [PubMed: 14570922] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, INDUCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000253 Genomic DNA. Translation: ABD30204.1.
RefSeq	YP_499634.1.
3D structure databases
SMR	Q2FZE2. Positions 1-107.
ModBase	Search...
Genome annotation databases
GeneID	3919250.
GenomeReviews	Gene locus SAOUHSC_01089 in contig CP000253_GR.
KEGG	sao:SAOUHSC_01089.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FZE2.
OMA	Q2FZE2. RDEGTSE.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_01089-MON.
Family and domain databases
HAMAP	MF_01272. [Tree]
InterPro	IPR007138. Antibiotic_mOase. [Graphical view]
Pfam	PF03992. ABM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISDG_STAA8

Accession

Primary (citable) accession number: Q2FZE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)