Q2FZE2 (HDOX1_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme-degrading monooxygenase 1
EC=1.14.99.-

Alternative name(s):
Heme oxygenase 1
Iron-regulated surface determinant 1
Iron-responsive surface determinant 1

Gene names

Name:	isdG
Ordered Locus Names:	SAOUHSC_01089

Organism

Staphylococcus aureus (strain NCTC 8325) [Reference proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the oxo-bilirubin chromophore staphylobilin (a mixture of the linear tetrapyrroles 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin) in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron By similarity. Ref.2
Catalytic activity	Heme + 3 AH2 + 3 O2 = staphylobilins + Fe2+ + CO + 3 A + 3 H2O. Ref.2
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Ref.2.
Induction	Transcriptionally regulated by iron and the ferric uptake repressor (fur) protein. Ref.2
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heme catabolic process Inferred from electronic annotation. Source: HAMAP iron assimilation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme binding Inferred from electronic annotation. Source: HAMAP heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 107	107	Heme-degrading monooxygenase 1 HAMAP-Rule MF_01272		PRO_0000270093
Regions
	Region	22 – 29	8	Heme binding By similarity
Sites
	Metal binding	7	1	Iron By similarity
	Metal binding	77	1	Iron (heme axial ligand) By similarity
	Site	67	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FZE2 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: DB13A134D5EC4FF0	FASTA	107	12,546
	10 20 30 40 50 60 MKFMAENRLT LTKGTAKDII ERFYTRHGIE TLEGFDGMFV TQTLEQEDFD EVKILTVWKS 70 80 90 100 KQAFTDWLKS DVFKAAHKHV RSKNEDESSP IINNKVITYD IGYSYMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.
[2]	"IsdG and IsdI, heme-degrading enzymes in the cytoplasm of Staphylococcus aureus." Skaar E.P., Gaspar A.H., Schneewind O. J. Biol. Chem. 279:436-443(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, INDUCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000253 Genomic DNA. Translation: ABD30204.1.
RefSeq	YP_499634.1. NC_007795.1.
3D structure databases
ProteinModelPortal	Q2FZE2.
SMR	Q2FZE2. Positions 1-107.
ModBase	Search...
Protein-protein interaction databases
STRING	93061.SAOUHSC_01089.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD30204; ABD30204; SAOUHSC_01089.
GeneID	3919250.
KEGG	sao:SAOUHSC_01089.
PATRIC	19579679. VBIStaAur99865_0998.
Phylogenomic databases
eggNOG	COG2329.
HOGENOM	HOG000008026.
KO	K00510.
OMA	GEGERIM.
ProtClustDB	PRK13312.
Enzyme and pathway databases
BioCyc	SAUR93061:GIWJ-1047-MONOMER.
Family and domain databases
HAMAP	MF_01272. Heme_degrading_monooxygenase.
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. IPR023953. Heme-degrad_mOase. [Graphical view]
Pfam	PF03992. ABM. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HDOX1_STAA8

Accession

Primary (citable) accession number: Q2FZE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 21, 2006
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program