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Protein

Ribonuclease HIII

Gene

rnhC

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mn2+UniRule annotation, Mg2+UniRule annotationNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011Divalent metal cationUniRule annotation
Metal bindingi102 – 1021Divalent metal cationUniRule annotation
Metal bindingi206 – 2061Divalent metal cationUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-1052-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HIIIUniRule annotation (EC:3.1.26.4UniRule annotation)
Short name:
RNase HIIIUniRule annotation
Gene namesi
Name:rnhCUniRule annotation
Ordered Locus Names:SAOUHSC_01095
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Ribonuclease HIIIPRO_1000031237Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01095.

Structurei

3D structure databases

ProteinModelPortaliQ2FZD7.
SMRiQ2FZD7. Positions 24-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family. RnhC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107FSS. Bacteria.
COG1039. LUCA.
HOGENOMiHOG000292531.
KOiK03471.
OMAiTGDYFGP.
OrthoDBiEOG6W723H.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00053. RNase_HIII.
InterProiIPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR004641. RNase_HIII.
IPR024568. RNase_HIII_N.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF11858. DUF3378. 1 hit.
PF01351. RNase_HII. 1 hit.
[Graphical view]
PIRSFiPIRSF037748. RnhC. 1 hit.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00716. rnhC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2FZD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANIVFKLSD KDITTLMSRI SFDTENLPQG MKARAKYQNT TVNIYQSGKV
60 70 80 90 100
MFQGNHAEAV SEELLPQHSQ LNTNKTKKKN MANSFLEQTL MYDQFNCIGS
110 120 130 140 150
DEAGSGDYFG PLTVCAAFVT KEHVPILKTL GVDDSKKLTD TKIVELAEQL
160 170 180 190 200
VTFIPHSLLT LHNEKYNIQQ AKGWTQVKMK AALHNEAIKN VLEKIDSSQL
210 220 230 240 250
DYIVIDQFAK REVYSHYALS DIPLPKKTKF ETKGESKSLA IAVASIISRY
260 270 280 290 300
AFVTYMDQIS KNINMTIPKG AGAKVDVIAA KIIKKYGLSR LDTISKKHFK
310
NREKAQKILK PL
Length:312
Mass (Da):35,055
Last modified:March 21, 2006 - v1
Checksum:iFFB2A5B878C599C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD30209.1.
RefSeqiYP_499639.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD30209; ABD30209; SAOUHSC_01095.
GeneIDi3920737.
KEGGisao:SAOUHSC_01095.
PATRICi19579689. VBIStaAur99865_1003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD30209.1.
RefSeqiYP_499639.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ2FZD7.
SMRiQ2FZD7. Positions 24-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01095.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD30209; ABD30209; SAOUHSC_01095.
GeneIDi3920737.
KEGGisao:SAOUHSC_01095.
PATRICi19579689. VBIStaAur99865_1003.

Phylogenomic databases

eggNOGiENOG4107FSS. Bacteria.
COG1039. LUCA.
HOGENOMiHOG000292531.
KOiK03471.
OMAiTGDYFGP.
OrthoDBiEOG6W723H.

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-1052-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00053. RNase_HIII.
InterProiIPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR004641. RNase_HIII.
IPR024568. RNase_HIII_N.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF11858. DUF3378. 1 hit.
PF01351. RNase_HII. 1 hit.
[Graphical view]
PIRSFiPIRSF037748. RnhC. 1 hit.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00716. rnhC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Staphylococcus aureus NCTC 8325 genome."
    Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
    (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 8325.

Entry informationi

Entry nameiRNH3_STAA8
AccessioniPrimary (citable) accession number: Q2FZD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 21, 2006
Last modified: July 6, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.