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Q2FZ93 (MRAY_STAA8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospho-N-acetylmuramoyl-pentapeptide-transferase
EC=2.7.8.13
Alternative name(s):
UDP-MurNAc-pentapeptide phosphotransferase
Gene names
Name:mraY
Ordered Locus Names:SAOUHSC_01146
OrganismStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifier93061 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity. HAMAP MF_00038

Catalytic activity

UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038

Subcellular location

Cell membrane; Multi-pass membrane protein HAMAP MF_00038.

Sequence similarities

Belongs to the glycosyltransferase 4 family. MraY subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 321321Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038
PRO_0000247938

Regions

Transmembrane1 – 2121Helical HAMAP MF_00038
Topological domain22 – 5231Cytoplasmic Potential
Transmembrane53 – 6917Helical HAMAP MF_00038
Topological domain70 – 756Extracellular Potential
Transmembrane76 – 9116Helical HAMAP MF_00038
Topological domain92 – 11120Cytoplasmic Potential
Transmembrane112 – 13322Helical HAMAP MF_00038
Topological domain134 – 15017Extracellular Potential
Transmembrane151 – 16515Helical HAMAP MF_00038
Topological domain166 – 17611Cytoplasmic Potential
Transmembrane177 – 19721Helical HAMAP MF_00038
Topological domain198 – 2025Extracellular Potential
Transmembrane203 – 21614Helical HAMAP MF_00038
Topological domain217 – 23216Cytoplasmic Potential
Transmembrane233 – 24614Helical HAMAP MF_00038
Topological domain247 – 2493Extracellular Potential
Transmembrane250 – 26819Helical HAMAP MF_00038
Topological domain269 – 30032Cytoplasmic Potential
Transmembrane301 – 32020Helical HAMAP MF_00038
Topological domain3211Extracellular Potential

Experimental info

Sequence conflict1041Q → P in AAC45625. Ref.1
Sequence conflict2541F → I in AAC45625. Ref.1
Sequence conflict299 – 32123KVVTV…WIGVH → ESTYSILGCWSDFRFNRFMD WSALRCLIIQG in AAC45625. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q2FZ93 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 0A2F82D042255848

FASTA32135,232
        10         20         30         40         50         60 
MIFVYALLAL VITFVLVPVL IPTLKRMKFG QSIREEGPQS HMKKTGTPTM GGLTFLLSIV 

        70         80         90        100        110        120 
ITSLVAIIFV DQANPIILLL FVTIGFGLIG FIDDYIIVVK KNNQGLTSKQ KFLAQIGIAI 

       130        140        150        160        170        180 
IFFVLSNVFH LVNFSTSIHI PFTNVAIPLS FAYVIFIVFW QVGFSNAVNL TDGLDGLATG 

       190        200        210        220        230        240 
LSIIGFTMYA IMSFVLGETA IGIFCIIMLF ALLGFLPYNI NPAKVFMGDT GSLALGGIFA 

       250        260        270        280        290        300 
TISIMLNQEL SLIFIGLVFV IETLSVMLQV ASFKLTGKRI FKMSPIHHHF ELIGWSEWKV 

       310        320 
VTVFWAVGLI SGLIGLWIGV H

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of cell wall-cell division gene clusters in pathogenic Gram-positive cocci."
Pucci M.J., Thanassi J.A., Discotto L.F., Kessler R.E., Dougherty T.J.
J. Bacteriol. 179:5632-5635(1997) [PubMed: 9287029] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Penicillin-binding protein 1 of Staphylococcus aureus is essential for growth."
Wada A., Watanabe H.
J. Bacteriol. 180:2759-2765(1998) [PubMed: 9573165] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The Staphylococcus aureus NCTC8325 genome."
Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 8325.
[4]"Topological analysis of the mraY protein catalyzing the first membrane step of peptidoglycan synthesis."
Bouhss A., Mengin-Lecreulx D., Le Beller D., Van Heijenoort J.
Mol. Microbiol. 34:576-585(1999) [PubMed: 10564498] [Abstract]
Cited for: TOPOLOGY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U94706 Genomic DNA. Translation: AAC45625.1.
AB007500 Genomic DNA. Translation: BAA22555.1.
CP000253 Genomic DNA. Translation: ABD30256.1.
RefSeqYP_499688.1. NC_007795.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ2FZ93.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000029344; EBSTAP00000028295; EBSTAG00000029342.
GeneID3920706.
GenomeReviewsGene locus SAOUHSC_01146 in contig CP000253_GR.
KEGGsao:SAOUHSC_01146.
PATRIC19579785. VBIStaAur99865_1051.

Phylogenomic databases

eggNOGCOG0472.
GeneTreeEBGT00050000024846.
HOGENOMHBG708263.
OMARFWIISM.
PhylomeDBQ2FZ93.
ProtClustDBPRK00108.

Enzyme and pathway databases

BioCycSAUR93061:SAOUHSC_01146-MONOMER.

Family and domain databases

HAMAPMF_00038. MraY.
[Tree]
InterProIPR000715. Glycosyl_transferase_4.
IPR003524. PNAcMuramoyl-5peptid_Trfase.
IPR018480. PNAcMuramoyl-5peptid_Trfase_CS.
[Graphical view]
KOK01000.
PANTHERPTHR22926. Glyco_trans_4. 1 hit.
PTHR22926:SF3. PNAcPpept_trans. 1 hit.
PfamPF00953. Glycos_transf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00445. MraY. 1 hit.
PROSITEPS01347. MRAY_1. 1 hit.
PS01348. MRAY_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMRAY_STAA8
AccessionPrimary (citable) accession number: Q2FZ93
Secondary accession number(s): O07322, O24815, P68784
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 21, 2006
Last modified: January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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