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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi490MagnesiumUniRule annotation1
Metal bindingi496MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR93061:G1G5Y-1169-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Synonyms:pnpA
Ordered Locus Names:SAOUHSC_01251
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002600601 – 698Polyribonucleotide nucleotidyltransferaseAdd BLAST698

Interactioni

Subunit structurei

Homodimer (Probable). Component of a possible RNA degradosome complex composed of cshA, eno, pfkA, pnp, rnjA, rnjB, rnpA and rny. Interacts specifically RNA helicase CshA and RNase J1.Curated1 Publication

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01251

Structurei

Secondary structure

1698
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Beta strandi15 – 24Combined sources10
Beta strandi28 – 36Combined sources9
Beta strandi39 – 47Combined sources9
Beta strandi53 – 55Combined sources3
Beta strandi59 – 61Combined sources3
Helixi67 – 70Combined sources4
Helixi87 – 99Combined sources13
Helixi100 – 102Combined sources3
Beta strandi111 – 119Combined sources9
Helixi126 – 140Combined sources15
Beta strandi141 – 143Combined sources3
Beta strandi150 – 157Combined sources8
Beta strandi160 – 164Combined sources5
Helixi167 – 170Combined sources4
Beta strandi174 – 181Combined sources8
Beta strandi183 – 196Combined sources14
Helixi198 – 226Combined sources29
Helixi240 – 252Combined sources13
Helixi255 – 259Combined sources5
Helixi264 – 278Combined sources15
Helixi292 – 316Combined sources25
Beta strandi331 – 335Combined sources5
Beta strandi338 – 349Combined sources12
Beta strandi352 – 361Combined sources10
Beta strandi380 – 385Combined sources6
Helixi388 – 391Combined sources4
Helixi402 – 416Combined sources15
Turni422 – 424Combined sources3
Beta strandi428 – 437Combined sources10
Helixi442 – 456Combined sources15
Beta strandi466 – 474Combined sources9
Beta strandi479 – 484Combined sources6
Helixi487 – 492Combined sources6
Beta strandi493 – 501Combined sources9
Beta strandi506 – 516Combined sources11
Helixi520 – 544Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5XEXX-ray2.20A/B/C/D/E/F1-553[»]
ProteinModelPortaliQ2FZ20
SMRiQ2FZ20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini557 – 616KHUniRule annotationAdd BLAST60
Domaini626 – 694S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62 Bacteria
COG1185 LUCA
HOGENOMiHOG000218327
KOiK00962
OMAiRYMHNYN

Family and domain databases

Gene3Di3.30.230.70, 2 hits
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR015848 PNPase_PH_RNA-bd_bac/org-type
IPR036456 PNPase_PH_RNA-bd_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF03726 PNPase, 1 hit
PF01138 RNase_PH, 2 hits
PF03725 RNase_PH_C, 2 hits
PF00575 S1, 1 hit
PIRSFiPIRSF005499 PNPase, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF46915 SSF46915, 1 hit
SSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Q2FZ20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEKKVFKT EWAGRSLTIE TGQLAKQANG AVLVRYGDTV VLSTATASKE
60 70 80 90 100
PRDGDFFPLT VNYEEKMYAA GKIPGGFKKR EGRPGDDATL TARLIDRPIR
110 120 130 140 150
PLFPKGYKHD VQIMNMVLSA DPDCSPQMAA MIGSSMALSV SDIPFQGPIA
160 170 180 190 200
GVNVGYIDGK YIINPTVEEK EVSRLDLEVA GHKDAVNMVE AGASEITEQE
210 220 230 240 250
MLEAIFFGHE EIQRLVDFQQ QIVDHIQPVK QEFIPAERDE ALVERVKSLT
260 270 280 290 300
EEKGLKETVL TFDKQQRDEN LDNLKEEIVN EFIDEEDPEN ELLIKEVYAI
310 320 330 340 350
LNELVKEEVR RLIADEKIRP DGRKPDEIRP LDSEVGILPR THGSGLFTRG
360 370 380 390 400
QTQALSVLTL GALGDYQLID GLGPEEEKRF MHHYNFPNFS VGETGPVRAP
410 420 430 440 450
GRREIGHGAL GERALKYIIP DTADFPYTIR IVSEVLESNG SSSQASICGS
460 470 480 490 500
TLALMDAGVP IKAPVAGIAM GLVTREDSYT ILTDIQGMED ALGDMDFKVA
510 520 530 540 550
GTKEGITAIQ MDIKIDGLTR EIIEEALEQA RRGRLEIMNH MLQTIDQPRT
560 570 580 590 600
ELSAYAPKVV TMTIKPDKIR DVIGPGGKKI NEIIDETGVK LDIEQDGTIF
610 620 630 640 650
IGAVDQAMIN RAREIIEEIT REAEVGQTYQ ATVKRIEKYG AFVGLFPGKD
660 670 680 690
ALLHISQISK NRIEKVEDVL KIGDTIEVKI TEIDKQGRVN ASHRALEE
Length:698
Mass (Da):77,362
Last modified:March 21, 2006 - v1
Checksum:i6EABFB86EDFBFB2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA Translation: ABD30352.1
RefSeqiWP_000076690.1, NC_007795.1
YP_499784.1, NC_007795.1

Genome annotation databases

EnsemblBacteriaiABD30352; ABD30352; SAOUHSC_01251
GeneIDi3919982
KEGGisao:SAOUHSC_01251
PATRICifig|93061.5.peg.1145

Similar proteinsi

Entry informationi

Entry nameiPNP_STAA8
AccessioniPrimary (citable) accession number: Q2FZ20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: March 21, 2006
Last modified: March 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health