Q2FXX8 (MTNN_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Short name=MTA/SAH nucleosidase
Short name=MTAN
EC=3.2.2.9

Alternative name(s):
5'-methylthioadenosine nucleosidase
Short name=MTA nucleosidase
S-adenosylhomocysteine nucleosidase
Short name=AdoHcy nucleosidase
Short name=SAH nucleosidase
Short name=SRH nucleosidase

Gene names

Name:	mtnN
Ordered Locus Names:	SAOUHSC_01702

Organism

Staphylococcus aureus (strain NCTC 8325)

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684 S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: EC methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684		PRO_0000359372
Regions
	Region	172 – 173	2	Substrate binding By similarity
Sites
	Active site	11	1	Proton acceptor By similarity
	Binding site	77	1	Substrate; via amide nitrogen By similarity
	Binding site	151	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	196	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FXX8 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: C756E9386E9B19DD	FASTA	228	24,534
	10 20 30 40 50 60 MIGIIGAMEE EVTILKNKLT QLSEISVAHV KFYTGILKDR EVVITQSGIG KVNAAISTTL 70 80 90 100 110 120 LINKFKPDVI INTGSAGALD ESLNVGDVLI SDDVKYHDAD ATAFGYEYGQ IPQMPVAFQS 130 140 150 160 170 180 SKPLIEKVSQ VVQQQQLTAK VGLIVSGDSF IGSVEQRQKI KKAFPNAMAV EMEATAIAQT 190 200 210 220 CYQFNVPFVV VRAVSDLANG EAEMSFEAFL EKAAVSSSQT VEALVSQL

References

[1]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000253 Genomic DNA. Translation: ABD30776.1.
RefSeq	YP_500212.1. NC_007795.1.
3D structure databases
HSSP	HSSP built from PDB template 1ZOS based on UniProtKB Q8DQ16.
ProteinModelPortal	Q2FXX8.
SMR	Q2FXX8. Positions 2-227.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FXX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000028094; EBSTAP00000027045; EBSTAG00000028093.
GeneID	3921092.
GenomeReviews	Gene locus SAOUHSC_01702 in contig CP000253_GR.
KEGG	sao:SAOUHSC_01702.
PATRIC	19580781. VBIStaAur99865_1551.
Phylogenomic databases
eggNOG	COG0775.
GeneTree	EBGT00050000025335.
HOGENOM	HBG367723.
OMA	LEHFPTM.
PhylomeDB	Q2FXX8.
ProtClustDB	CLSK885455.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_01702-MONOMER.
Family and domain databases
HAMAP	MF_01684. Salvage_tnN. [Tree]
InterPro	IPR010049. MTA_SAH_Nsdase. IPR018017. Nucleoside_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
KO	K01243.
PANTHER	PTHR21234. PNP_UDP. 1 hit. PTHR21234:SF6. PTHR21234:SF6. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNN_STAA8

Accession

Primary (citable) accession number: Q2FXX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program