Q2FXU4 (SYH_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidine--tRNA ligase
EC=6.1.1.21

Alternative name(s):
Histidyl-tRNA synthetase
Short name=HisRS

Gene names

Name:	hisS
Ordered Locus Names:	SAOUHSC_01738

Organism

Staphylococcus aureus (strain NCTC 8325) [Reference proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP-Rule MF_00127
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histidyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP histidine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Histidine--tRNA ligase HAMAP-Rule MF_00127		PRO_1000016460

Sequences

	Sequence		Length	Mass (Da)
	Q2FXU4 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 3B79521695278CA4	FASTA	420	48,283
	10 20 30 40 50 60 MIKIPRGTQD ILPEDSKKWR YIENQLDELM TFYNYKEIRT PIFESTDLFA RGVGDSTDVV 70 80 90 100 110 120 QKEMYTFKDK GDRSITLRPE GTAAVVRSYI EHKMQGNPNQ PIKLYYNGPM FRYERKQKGR 130 140 150 160 170 180 YRQFNQFGVE AIGAENPSVD AEVLAMVMHI YQSFGLKHLK LVINSVGDMA SRKEYNEALV 190 200 210 220 230 240 KHFEPVIHEF CSDCQSRLHT NPMRILDCKV DRDKEAIKTA PRITDFLNEE SKAYYEQVKA 250 260 270 280 290 300 YLDDLGIPYI EDPNLVRGLD YYTHTAFELM MDNPNYDGAI TTLCGGGRYN GLLELLDGPS 310 320 330 340 350 360 ETGIGFALSI ERLLLALEEE GIELDIEENL DLFIVTMGDQ ADRYAVKLLN HLRHNGIKAD 370 380 390 400 410 420 KDYLQRKIKG QMKQADRLGA KFTIVIGDQE LENNKIDVKN MTTGESETIE LDALVEYFKK

References

[1]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000253 Genomic DNA. Translation: ABD30810.1.
RefSeq	YP_500246.1. NC_007795.1.
3D structure databases
ProteinModelPortal	Q2FXU4.
SMR	Q2FXU4. Positions 1-420.
ModBase	Search...
Protein-protein interaction databases
STRING	93061.SAOUHSC_01738.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD30810; ABD30810; SAOUHSC_01738.
GeneID	3921087.
KEGG	sao:SAOUHSC_01738.
PATRIC	19580847. VBIStaAur99865_1584.
Phylogenomic databases
eggNOG	COG0124.
HOGENOM	HOG000018072.
KO	K01892.
OMA	NMFRYEE.
ProtClustDB	PRK00037.
Enzyme and pathway databases
BioCyc	SAUR93061:GIWJ-1660-MONOMER.
Family and domain databases
Gene3D	3.40.50.800. 1 hit.
HAMAP	MF_00127. His_tRNA_synth.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR015807. His-tRNA-ligase. IPR004516. HisRS/HisZ. [Graphical view]
PANTHER	PTHR11476. PTHR11476. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001549. His-tRNA_synth. 1 hit.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00442. hisS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYH_STAA8

Accession

Primary (citable) accession number: Q2FXU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 21, 2006
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program