ID   HEM2_STAA8              Reviewed;         324 AA.
AC   Q2FXR3; P50915;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=Delta-aminolevulinic acid dehydratase;
DE            Short=ALADH;
DE            Short=ALAD;
DE            EC=4.2.1.24;
DE   AltName: Full=Porphobilinogen synthase;
GN   Name=hemB; OrderedLocusNames=SAOUHSC_01772;
OS   Staphylococcus aureus (strain NCTC 8325).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=98019093; PubMed=9358061; DOI=10.1016/S0378-1119(97)00372-7;
RA   Kafala B., Sasarman A.;
RT   "Isolation of the Staphylococcus aureus hemCDBL gene cluster coding
RT   for early steps in heme biosynthesis.";
RL   Gene 199:231-239(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W.,
RA   Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC8325 genome.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: 2 5-aminolevulinate = porphobilinogen + 2
CC       H(2)O.
CC   -!- COFACTOR: Zinc (By similarity).
CC   -!- PATHWAY: Porphyrin metabolism; protoporphyrin-IX biosynthesis;
CC       coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
CC   -!- SUBUNIT: Homooctamer (By similarity).
CC   -!- SIMILARITY: Belongs to the ALADH family.
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DR   EMBL; U89396; AAC45835.1; -; Genomic_DNA.
DR   EMBL; CP000253; ABD30841.1; -; Genomic_DNA.
DR   RefSeq; YP_500277.1; -.
DR   GeneID; 3919690; -.
DR   GenomeReviews; CP000253_GR; SAOUHSC_01772.
DR   KEGG; sao:SAOUHSC_01772; -.
DR   HOGENOM; Q2FXR3; -.
DR   OMA; Q2FXR3; AESTPQF.
DR   BioCyc; SAUR93061:SAOUHSC_01772-MON; -.
DR   GO; GO:0004655; F:porphobilinogen synthase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006779; P:porphyrin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001731; 4pyrrol_synth_porphobiln_synth.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   PANTHER; PTHR11458; AlaD_dehydratase; 1.
DR   Pfam; PF00490; ALAD; 1.
DR   PIRSF; PIRSF001415; Porphbilin_synth; 1.
DR   PRINTS; PR00144; DALDHYDRTASE.
DR   ProDom; PD002304; AlaD_dehydratase; 1.
DR   PROSITE; PS00169; D_ALA_DEHYDRATASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lyase; Porphyrin biosynthesis; Zinc.
FT   CHAIN         1    323       Delta-aminolevulinic acid dehydratase.
FT                                /FTId=PRO_0000247939.
FT   REGION      115    133       Zinc-binding (By similarity).
FT   ACT_SITE    248    248       By similarity.
FT   CONFLICT    314    315       FA -> C (in Ref. 1; AAC45835).
FT   CONFLICT    324    324       K -> N (in Ref. 1; AAC45835).
SQ   SEQUENCE   324 AA;  36583 MW;  BF24378E01E7C93E CRC64;
     MKFDRHRRLR SSATMRDMVR ENHVRKEDLI YPIFVVEKDD VKKEIKSLPG VYQISLNLLE
     SELKEAYDLG IRAIMFFGVP NSKDDIGTGA YIHDGVIQQA TRIAKKMYDD LLIVADTCLC
     EYTDHGHCGV IDDHTHDVDN DKSLPLLVKT AISQVEAGAD IIAPSNMMDG FVAEIRRGLD
     EAGYYNIPIM SYGVKYASSF FGPFRDAADS APSFGDRKTY QMDPANRLEA LRELESDLKE
     GCDMMIVKPA LSYLDIVRDV KNHTNVPVVA YNVSGEYSMT KAAAQNGWID EERVVMEQMV
     SMKRAGADMI ITYFAKDICR YLDK
//