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Reviewed, UniProtKB/Swiss-Prot Q2FXR3 (HEM2_STAA8)

Last modified January 19, 2010. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase
Gene names
Name: hemB
Ordered Locus Names: SAOUHSC_01772
OrganismStaphylococcus aureus (strain NCTC 8325) [Complete proteome] [HAMAP]
Taxonomic identifier93061 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesStaphylococcus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactor

Zinc By similarity.

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.

Subunit structure

Homooctamer By similarity.

Sequence similarities

Belongs to the ALADH family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   LigandZinc
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processporphyrin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionporphobilinogen synthase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Delta-aminolevulinic acid dehydratase
PRO_0000247939

Regions

Region115 – 13319Zinc-binding By similarity

Sites

Active site2481 By similarity

Experimental info

Sequence conflict314 – 3152FA → C in AAC45835. Ref.1
Sequence conflict3241K → N in AAC45835. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q2FXR3-1 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: BF24378E01E7C93E

FASTA32436,583
        10         20         30         40         50         60 
MKFDRHRRLR SSATMRDMVR ENHVRKEDLI YPIFVVEKDD VKKEIKSLPG VYQISLNLLE 

        70         80         90        100        110        120 
SELKEAYDLG IRAIMFFGVP NSKDDIGTGA YIHDGVIQQA TRIAKKMYDD LLIVADTCLC 

       130        140        150        160        170        180 
EYTDHGHCGV IDDHTHDVDN DKSLPLLVKT AISQVEAGAD IIAPSNMMDG FVAEIRRGLD 

       190        200        210        220        230        240 
EAGYYNIPIM SYGVKYASSF FGPFRDAADS APSFGDRKTY QMDPANRLEA LRELESDLKE 

       250        260        270        280        290        300 
GCDMMIVKPA LSYLDIVRDV KNHTNVPVVA YNVSGEYSMT KAAAQNGWID EERVVMEQMV 

       310        320 
SMKRAGADMI ITYFAKDICR YLDK 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of the Staphylococcus aureus hemCDBL gene cluster coding for early steps in heme biosynthesis."
Kafala B., Sasarman A.
Gene 199:231-239(1997) [PubMed: 9358061] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The Staphylococcus aureus NCTC8325 genome."
Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U89396 Genomic DNA. Translation: AAC45835.1.
CP000253 Genomic DNA. Translation: ABD30841.1.
RefSeqYP_500277.1.

3D structure databases

SMRQ2FXR3. Positions 3-320.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2FXR3.

Genome annotation databases

GeneID3919690.
GenomeReviewsGene locus SAOUHSC_01772 in contig CP000253_GR.
KEGGsao:SAOUHSC_01772.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0113.
HOGENOMHBG285270.
OMAITYFAKE.

Enzyme and pathway databases

BioCycSAUR93061:SAOUHSC_01772-MONOMER.

Family and domain databases

InterProIPR001731. 4pyrrol_synth_porphobiln_synth.
IPR013785. Aldolase_TIM.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHERPTHR11458. AlaD_dehydratase. 1 hit.
PfamPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSPR00144. DALDHYDRTASE.
PROSITEPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM2_STAA8
AccessionPrimary (citable) accession number: Q2FXR3
Secondary accession number(s): P50915
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 21, 2006
Last modified: January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents