Reviewed, UniProtKB/Swiss-Prot Q2FXR3 (HEM2_STAA8)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	SAOUHSC_01772

Organism

Staphylococcus aureus (strain NCTC 8325) [Complete proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Zinc By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Ligand	Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	porphobilinogen synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Delta-aminolevulinic acid dehydratase		PRO_0000247939
Regions
	Region	115 – 133	19	Zinc-binding By similarity
Sites
	Active site	248	1	By similarity
Experimental info
	Sequence conflict	314 – 315	2	FA → C in AAC45835. Ref.1
	Sequence conflict	324	1	K → N in AAC45835. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q2FXR3-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: BF24378E01E7C93E	FASTA	324	36,583
	10 20 30 40 50 60 MKFDRHRRLR SSATMRDMVR ENHVRKEDLI YPIFVVEKDD VKKEIKSLPG VYQISLNLLE 70 80 90 100 110 120 SELKEAYDLG IRAIMFFGVP NSKDDIGTGA YIHDGVIQQA TRIAKKMYDD LLIVADTCLC 130 140 150 160 170 180 EYTDHGHCGV IDDHTHDVDN DKSLPLLVKT AISQVEAGAD IIAPSNMMDG FVAEIRRGLD 190 200 210 220 230 240 EAGYYNIPIM SYGVKYASSF FGPFRDAADS APSFGDRKTY QMDPANRLEA LRELESDLKE 250 260 270 280 290 300 GCDMMIVKPA LSYLDIVRDV KNHTNVPVVA YNVSGEYSMT KAAAQNGWID EERVVMEQMV 310 320 SMKRAGADMI ITYFAKDICR YLDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the Staphylococcus aureus hemCDBL gene cluster coding for early steps in heme biosynthesis." Kafala B., Sasarman A. Gene 199:231-239(1997) [PubMed: 9358061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U89396 Genomic DNA. Translation: AAC45835.1. CP000253 Genomic DNA. Translation: ABD30841.1.
RefSeq	YP_500277.1.
3D structure databases
SMR	Q2FXR3. Positions 3-320.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FXR3.
Genome annotation databases
GeneID	3919690.
GenomeReviews	Gene locus SAOUHSC_01772 in contig CP000253_GR.
KEGG	sao:SAOUHSC_01772.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0113.
HOGENOM	HBG285270.
OMA	ITYFAKE.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_01772-MONOMER.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_STAA8

Accession

Primary (citable) accession number: Q2FXR3
Secondary accession number(s): P50915

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 21, 2006
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)