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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43S-adenosyl-L-methionineUniRule annotation1
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei95S-adenosyl-L-methionineUniRule annotation1
Active sitei117By similarity1
Binding sitei117S-adenosyl-L-methionineUniRule annotation1
Binding sitei121SubstrateUniRule annotation1
Binding sitei153SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSAUR93061:G1G5Y-1734-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:SAOUHSC_01865
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002882321 – 214tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST214

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01865

Structurei

3D structure databases

ProteinModelPortaliQ2FXI2
SMRiQ2FXI2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 193Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000251689
KOiK03439
OMAiWITFPDP

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q2FXI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVRYKPWAE DYLKDHPELV DMDGQHAGKM TEWFDKTQPI HIEIGSGMGQ
60 70 80 90 100
FITTLAAQNP HINYISMERE KSIVYKVLDK VKEMGLTNLK IICNDAIELN
110 120 130 140 150
EYFKDGEVSR IYLNFSDPWP KNRHAKRRLT YHTFLALYQQ ILNDEGDLHF
160 170 180 190 200
KTDNRGLFAY SLESMSQFGM YFTKINLNLH QEDDGSNILT EYEKKFSDKG
210
SRIYRMEAKF HSQK
Length:214
Mass (Da):25,276
Last modified:March 21, 2006 - v1
Checksum:i92987FE746A9DDD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA Translation: ABD30930.1
RefSeqiWP_001266157.1, NC_007795.1
YP_500368.1, NC_007795.1

Genome annotation databases

EnsemblBacteriaiABD30930; ABD30930; SAOUHSC_01865
GeneIDi3921754
KEGGisao:SAOUHSC_01865
PATRICifig|93061.5.peg.1697

Similar proteinsi

Entry informationi

Entry nameiTRMB_STAA8
AccessioniPrimary (citable) accession number: Q2FXI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 21, 2006
Last modified: March 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome