Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine protease SplA

Gene

splA

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74Charge relay systemBy similarity1
Active sitei113Charge relay systemBy similarity1
Active sitei189Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease SplA (EC:3.4.21.-)
Gene namesi
Name:splA
Ordered Locus Names:SAOUHSC_01942
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 351 PublicationAdd BLAST35
ChainiPRO_000035953336 – 235Serine protease SplAAdd BLAST200

Expressioni

Developmental stagei

Maximally expressed during early stationary phase.1 Publication

Inductioni

Positively regulated by agr (accessory gene regulator).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01942.

Structurei

Secondary structure

1235
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 43Combined sources5
Helixi50 – 52Combined sources3
Beta strandi53 – 56Combined sources4
Beta strandi59 – 65Combined sources7
Beta strandi68 – 71Combined sources4
Helixi73 – 82Combined sources10
Beta strandi86 – 89Combined sources4
Beta strandi92 – 94Combined sources3
Beta strandi102 – 107Combined sources6
Beta strandi109 – 113Combined sources5
Beta strandi115 – 119Combined sources5
Helixi129 – 132Combined sources4
Beta strandi147 – 152Combined sources6
Helixi156 – 158Combined sources3
Beta strandi163 – 174Combined sources12
Beta strandi177 – 180Combined sources4
Beta strandi192 – 194Combined sources3
Beta strandi200 – 207Combined sources8
Beta strandi215 – 220Combined sources6
Helixi223 – 231Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W7SX-ray1.80A/B/C/D36-235[»]
2W7UX-ray2.43A/B/C/D36-235[»]
3UFAX-ray1.80A/B36-235[»]
4MVNX-ray1.70A/B/C/D36-235[»]
ProteinModelPortaliQ2FXC2.
SMRiQ2FXC2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ2FXC2.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S1B family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108CHM. Bacteria.
COG3591. LUCA.
HOGENOMiHOG000107379.
KOiK01318.
OMAiNAQFEVV.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR008256. Peptidase_S1B.
IPR008353. Peptidase_S1B_tx.
IPR001254. Trypsin_dom.
IPR028301. V8_his_AS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR01774. EXFOLTOXIN.
PR00839. V8PROTEASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00672. V8_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2FXC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKNVMVKGL TALTILTSLG FAENISNQPH SIAKAEKNVK EITDATKEPY
60 70 80 90 100
NSVVAFVGGT GVVVGKNTIV TNKHIAKSND IFKNRVSAHH SSKGKGGGNY
110 120 130 140 150
DVKDIVEYPG KEDLAIVHVH ETSTEGLNFN KNVSYTKFAD GAKVKDRISV
160 170 180 190 200
IGYPKGAQTK YKMFESTGTI NHISGTFMEF DAYAQPGNSG SPVLNSKHEL
210 220 230
IGILYAGSGK DESEKNFGVY FTPQLKEFIQ NNIEK
Length:235
Mass (Da):25,549
Last modified:March 21, 2006 - v1
Checksum:iDBC6B23C6648BCF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF271715 Genomic DNA. Translation: AAF97925.1.
CP000253 Genomic DNA. Translation: ABD31004.1.
RefSeqiWP_001039435.1. NC_007795.1.
YP_500442.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD31004; ABD31004; SAOUHSC_01942.
GeneIDi3921025.
KEGGisao:SAOUHSC_01942.
PATRICi19581213. VBIStaAur99865_1767.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF271715 Genomic DNA. Translation: AAF97925.1.
CP000253 Genomic DNA. Translation: ABD31004.1.
RefSeqiWP_001039435.1. NC_007795.1.
YP_500442.1. NC_007795.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W7SX-ray1.80A/B/C/D36-235[»]
2W7UX-ray2.43A/B/C/D36-235[»]
3UFAX-ray1.80A/B36-235[»]
4MVNX-ray1.70A/B/C/D36-235[»]
ProteinModelPortaliQ2FXC2.
SMRiQ2FXC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD31004; ABD31004; SAOUHSC_01942.
GeneIDi3921025.
KEGGisao:SAOUHSC_01942.
PATRICi19581213. VBIStaAur99865_1767.

Phylogenomic databases

eggNOGiENOG4108CHM. Bacteria.
COG3591. LUCA.
HOGENOMiHOG000107379.
KOiK01318.
OMAiNAQFEVV.

Miscellaneous databases

EvolutionaryTraceiQ2FXC2.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR008256. Peptidase_S1B.
IPR008353. Peptidase_S1B_tx.
IPR001254. Trypsin_dom.
IPR028301. V8_his_AS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR01774. EXFOLTOXIN.
PR00839. V8PROTEASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00672. V8_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPLA_STAA8
AccessioniPrimary (citable) accession number: Q2FXC2
Secondary accession number(s): Q9KH51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.