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Protein

Diacylglycerol kinase

Gene

dagK

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. This ion appears to have a structural role and is required for catalytic activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Binding sitei94 – 941ATPPROSITE-ProRule annotation
Metal bindingi213 – 2131Magnesium; via carbonyl oxygenBy similarity
Metal bindingi216 – 2161MagnesiumBy similarity
Metal bindingi218 – 2181Magnesium; via carbonyl oxygenBy similarity
Active sitei273 – 2731Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 145ATPPROSITE-ProRule annotation
Nucleotide bindingi67 – 737ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-2059-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Ordered Locus Names:SAOUHSC_02114
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000008816 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Diacylglycerol kinasePRO_0000386488Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi93061.SAOUHSC_02114.

Structurei

3D structure databases

ProteinModelPortaliQ2FWZ2.
SMRiQ2FWZ2. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 132132DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the diacylglycerol/lipid kinase family.Curated
Contains 1 DAGKc domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1597.
HOGENOMiHOG000261395.
KOiK07029.
OMAiPGEFVNL.
OrthoDBiEOG613098.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2FWZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKRARIIYN PTSGKELFKR ELPDALIKLE KAGYETSAYA TEKIGDATLE
60 70 80 90 100
AERAMHENYD VLIAAGGDGT LNEVVNGIAE KPNRPKLGVI PMGTVNDFGR
110 120 130 140 150
ALHIPNDIMG ALDVIIEGHS TKVDIGKMNN RYFINLAAGG QLTQVSYETP
160 170 180 190 200
SKLKSIVGPF AYYIKGFEML PQMKAVDLRI EYDGNVFQGE ALLFFLGLTN
210 220 230 240 250
SMAGFEKLVP DAKLDDGYFT LIIVEKSNLA ELGHIMTLAS RGEHTKHPKV
260 270 280 290 300
IYEKAKAINI SSFTDLQLNV DGEYGGKLPA NFLNLERHID VFAPNDIVNE
310
ELINNDHVDD NLIEE
Length:315
Mass (Da):34,887
Last modified:March 21, 2006 - v1
Checksum:iC5CC706B5E3A0F00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD31164.1.
RefSeqiWP_001231451.1. NC_007795.1.
YP_500606.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD31164; ABD31164; SAOUHSC_02114.
GeneIDi23197755.
3921186.
KEGGisao:SAOUHSC_02114.
PATRICi19581593. VBIStaAur99865_1918.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000253 Genomic DNA. Translation: ABD31164.1.
RefSeqiWP_001231451.1. NC_007795.1.
YP_500606.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ2FWZ2.
SMRiQ2FWZ2. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_02114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD31164; ABD31164; SAOUHSC_02114.
GeneIDi23197755.
3921186.
KEGGisao:SAOUHSC_02114.
PATRICi19581593. VBIStaAur99865_1918.

Phylogenomic databases

eggNOGiCOG1597.
HOGENOMiHOG000261395.
KOiK07029.
OMAiPGEFVNL.
OrthoDBiEOG613098.

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-2059-MONOMER.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Staphylococcus aureus NCTC 8325 genome."
    Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
    (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 8325.

Entry informationi

Entry nameiDAGK_STAA8
AccessioniPrimary (citable) accession number: Q2FWZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: March 21, 2006
Last modified: July 22, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.