Q2FWL2 (GCP_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp

Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein

Gene names

Name:	gcp
Ordered Locus Names:	SAOUHSC_02277

Organism

Staphylococcus aureus (strain NCTC 8325)

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. Critical mediator involved in the modification of cell wall peptidoglycan synthesis and/or cell division as well as in the positive regulation of the activities of different murein hydrolases. Essential for cell viability. Negatively affects the expression of lrgA. Positively affects cidA expression, maybe indirectly. May be an important chelator of excess zinc. Down-regulation of gcp eliminates penicillin- and vancomycin-caused cell lysis, inhibits several extracellular hydrolase activities, dramatically increasing tolerance to hydrolases and leads to a bacteriostatic effect. in the modification of cell wall peptidoglycan synthesis and/or cell division. Essential for cell viability By similarity. Ref.2 Ref.3
Subcellular location	Cytoplasm Potential HAMAP MF_01445.
Sequence similarities	Belongs to the KAE1 / YgjD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp HAMAP MF_01445		PRO_0000303551

Sequences

	Sequence		Length	Mass (Da)
	Q2FWL2 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: AB4C57F8D21132C6	FASTA	341	36,819
	10 20 30 40 50 60 MTKDILILAV ETSCDETSVS VIKNGRDILS NTVLSQIESH KRFGGVVPEV ASRHHVEGIT 70 80 90 100 110 120 ATINEALGDA DVSIEDIDAI AVTEGPGLIG ALLIGVNAAK ALAFAYDKPL IPVHHIAGHI 130 140 150 160 170 180 YANHIEEPLT FPLIALIVSG GHTELVYMKD HLSFEVIGET RDDAVGEAYD KVARTIGLNY 190 200 210 220 230 240 PGGPQVDRLA AEGEDTYSFP RVWLDKDSYD FSFSGLKSAV INQLHNQRQK NIPIIEANVA 250 260 270 280 290 300 TSFQNSVVEV LTFKAIQACK EYGVQRLIVA GGVASNKGLR QSLADQCKVN DIQLTIPSPK 310 320 330 340 LCTDNAAMIG VAGHYLYQQG RFADLALNGH SNIDLEEYSA E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.
[2]	"Identification of an essential glycoprotease in Staphylococcus aureus." Zheng L., Yang J., Landwehr C., Fan F., Ji Y. FEMS Microbiol. Lett. 245:279-285(2005) [PubMed: 15837383] [Abstract] Cited for: FUNCTION IN CELL VIABILITY.
[3]	"Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus." Zheng L., Yu C., Bayles K., Lasa I., Ji Y. J. Bacteriol. 189:2734-2742(2007) [PubMed: 17237169] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000253 Genomic DNA. Translation: ABD31315.1.
RefSeq	YP_500758.1. NC_007795.1.
3D structure databases
ProteinModelPortal	Q2FWL2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FWL2.
Protein family/group databases
MEROPS	M22.A01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000027974; EBSTAP00000026925; EBSTAG00000027973.
GeneID	3919152.
GenomeReviews	Gene locus SAOUHSC_02277 in contig CP000253_GR.
KEGG	sao:SAOUHSC_02277.
PATRIC	19581891. VBIStaAur99865_2067.
Phylogenomic databases
eggNOG	COG0533.
GeneTree	EBGT00050000024961.
HOGENOM	HBG304663.
OMA	PAVGVHH.
PhylomeDB	Q2FWL2.
ProtClustDB	PRK09604.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_02277-MONOMER.
Family and domain databases
HAMAP	MF_01445. gcp. [Tree]
InterPro	IPR022450. Pept_M22_O-sialoglycopept. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
KO	K01409.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR03723. Bact_gcp. 1 hit. TIGR00329. Gcp_kae1. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_STAA8

Accession

Primary (citable) accession number: Q2FWL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program