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Q2FW03 (TOP3_STAA8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:topB
Ordered Locus Names:SAOUHSC_02517
OrganismStaphylococcus aureus (strain NCTC 8325) [Reference proteome] [HAMAP]
Taxonomic identifier93061 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00953

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00953

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00953

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   LigandDNA-binding
Magnesium
Metal-binding
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711DNA topoisomerase 3 HAMAP-Rule MF_00953
PRO_0000286368

Regions

Domain2 – 135134Toprim
Region186 – 1916Interaction with DNA By similarity

Sites

Active site3051O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding81Magnesium; catalytic By similarity
Metal binding1041Magnesium; catalytic By similarity
Site601Interaction with DNA By similarity
Site1671Interaction with DNA By similarity
Site1751Interaction with DNA By similarity
Site3071Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2FW03 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: E83A8F21CFE6918A

FASTA71181,552
        10         20         30         40         50         60 
MKSLILAEKP SVARDIADAL QINQKRNGYF ENNQYIVTWA LGHLVTNATP EQYDKNLKEW 

        70         80         90        100        110        120 
RLEDLPIIPK YMKTVVIGKT SKQFKTVKAL ILDNKVKDII IATDAGREGE LVARLILDKV 

       130        140        150        160        170        180 
GNKKPIRRLW ISSVTKKAIQ QGFKNLKDGR QYNDLYYAAL ARSEADWIVG INATRALTTK 

       190        200        210        220        230        240 
YDAQLSLGRV QTPTIQLVNT RQQEINQFKP QQYFTLSLTV KGFDFQLESN QRYTNKETLE 

       250        260        270        280        290        300 
QMVNNLKNVD GKIKSVATKH KKSYPQSLYN LTDLQQDMYR RYKIGPKETL NTLQSLYERH 

       310        320        330        340        350        360 
KVVTYPRTDS NYLTTDMVDT MKERIQVTMA TTYKDQARPL MSKTFSSKMS IFNNQKVSDH 

       370        380        390        400        410        420 
HAIIPTEVRP VMSDLSNREL KLYDMIVERF LEALMPPHEY DAITVTLEVA GHTFVLKENV 

       430        440        450        460        470        480 
TTVLGFKSIR QGESITEMQQ PFSEGDEVKI SKTNIREHET TPPEYFNEGS LLKAMENPQN 

       490        500        510        520        530        540 
FIQLKDKKYA QTLKQTGGIG TVATRADIID KLFNMNAIES RDGKIKVTSK GKQILELAPE 

       550        560        570        580        590        600 
ELTSPLLTAQ WEEKLLLIER GKYQAKTFIN EMKDFTKDVV NGIKNSDRKY KHDNLTTTEC 

       610        620        630        640        650        660 
PTCGKFMIKV KTKNGQMLVC QDPSCKTKKN VQRKTNARCP NCKKKLTLFG KGKEAVYRCV 

       670        680        690        700        710 
CGHSETQAHM DQRMKSKSSG KVSRKEMKKY MNKNEGLDNN PFKDALKNLN L 

« Hide

References

[1]"The Staphylococcus aureus NCTC 8325 genome."
Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
(In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 8325.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000253 Genomic DNA. Translation: ABD31533.1.
RefSeqYP_500982.1. NC_007795.1.

3D structure databases

ProteinModelPortalQ2FW03.
SMRQ2FW03. Positions 4-630.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING93061.SAOUHSC_02517.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD31533; ABD31533; SAOUHSC_02517.
GeneID3920891.
KEGGsao:SAOUHSC_02517.
PATRIC19582330. VBIStaAur99865_2271.

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000086847.
KOK03169.
OMATKHDEYF.
OrthoDBEOG60CWM6.

Enzyme and pathway databases

BioCycSAUR93061:GIWJ-2450-MONOMER.

Family and domain databases

Gene3D1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00953. Topoisom_3_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01056. topB. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP3_STAA8
AccessionPrimary (citable) accession number: Q2FW03
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: March 21, 2006
Last modified: July 9, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families