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Protein

Nitrogenase iron protein

Gene

nifH

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.UniRule annotation

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi95Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi128Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMHUN323259:G1G5Z-844-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron proteinUniRule annotation (EC:1.18.6.1UniRule annotation)
Alternative name(s):
Nitrogenase Fe proteinUniRule annotation
Nitrogenase component IIUniRule annotation
Nitrogenase reductaseUniRule annotation
Gene namesi
Name:nifHUniRule annotation
Ordered Locus Names:Mhun_0793
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
Proteomesi
  • UP000001941 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002118741 – 280Nitrogenase iron proteinAdd BLAST280

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei98ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseUniRule annotation1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-98 inactivates the nitrogenase reductase and regulates nitrogenase activity.UniRule annotation

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_0793

Structurei

3D structure databases

ProteinModelPortaliQ2FUB7
SMRiQ2FUB7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00590 Archaea
COG1348 LUCA
HOGENOMiHOG000228825
KOiK02588
OMAiPGDIVCG
OrthoDBiPOG093Z00RZ

Family and domain databases

CDDicd02040 NifH, 1 hit
HAMAPiMF_00533 NifH, 1 hit
InterProiView protein in InterPro
IPR030655 NifH/chlL_CS
IPR000392 NifH/frxC
IPR005977 Nitrogenase_Fe_NifH
IPR027417 P-loop_NTPase
PANTHERiPTHR42864 PTHR42864, 1 hit
PfamiView protein in Pfam
PF00142 Fer4_NifH, 1 hit
PIRSFiPIRSF000363 Nitrogenase_iron, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00746 NIFH_FRXC_1, 1 hit
PS00692 NIFH_FRXC_2, 1 hit
PS51026 NIFH_FRXC_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q2FUB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQIALYGKG GIGKSTTSAN LSAALVNRGL SVMQIGCDPK RDSTRMLMKG
60 70 80 90 100
ILIPTVLDLI RERGEENLTL DDVVFTGYKG VRCVEAGGPE PGVGCAGRGI
110 120 130 140 150
IATFQLLERL SAFDEDIIVY DVLGDVVCGG FAMPMRKGYA QEIYLVTSGE
160 170 180 190 200
LMSLYAANNI CKAISRISQN VRQVCRLGGV ICNSRNLPDE EKLVGAFASE
210 220 230 240 250
VGSKIIAYIP RSGLVQYAEL NNQTVIEFAP DSSLSATYQS LAEEIMTNTD
260 270 280
FVIPKPLEIE ELEKLARSYL PTIDHQGINH
Length:280
Mass (Da):30,372
Last modified:March 21, 2006 - v1
Checksum:iCA9398B775AE1C4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA Translation: ABD40545.1
RefSeqiWP_011447824.1, NC_007796.1

Genome annotation databases

EnsemblBacteriaiABD40545; ABD40545; Mhun_0793
GeneIDi3924521
KEGGimhu:Mhun_0793

Similar proteinsi

Entry informationi

Entry nameiNIFH_METHJ
AccessioniPrimary (citable) accession number: Q2FUB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 21, 2006
Last modified: March 28, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health