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Protein

Enolase 3

Gene

eno3

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase 2 (eno2), Enolase 1 (eno1), Enolase 3 (eno3)
  5. Pyruvate kinase (Mhun_0465)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei143SubstrateUniRule annotation1
Binding sitei152SubstrateUniRule annotation1
Active sitei193Proton donorUniRule annotation1
Metal bindingi229MagnesiumUniRule annotation1
Metal bindingi273MagnesiumUniRule annotation1
Binding sitei273SubstrateUniRule annotation1
Metal bindingi300MagnesiumUniRule annotation1
Binding sitei300SubstrateUniRule annotation1
Active sitei325Proton acceptorUniRule annotation1
Binding sitei325Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei376SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 3UniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 3UniRule annotation
2-phosphoglycerate dehydratase 3UniRule annotation
Gene namesi
Name:eno3UniRule annotation
Ordered Locus Names:Mhun_2893
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
Proteomesi
  • UP000001941 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation

  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002671461 – 402Enolase 3Add BLAST402

Interactioni

Protein-protein interaction databases

STRINGi323259.Mhun_2893.

Structurei

3D structure databases

ProteinModelPortaliQ2FTN3.
SMRiQ2FTN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni352 – 355Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169. Archaea.
COG0148. LUCA.
HOGENOMiHOG000072173.
KOiK01689.
OMAiYVEDPFH.
OrthoDBiPOG093Z03D9.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2FTN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTITRAITLR TILDSRGRKT VEAEVRTEHG FGRAAAPGGA STGIHEAAVR
60 70 80 90 100
DPLLAIADAQ GQVIPHLIGI DSSDQIGVDS LLHEIDGTDN FSGIGANVAV
110 120 130 140 150
ALSLAMAKAA ADATNIPLWQ HIGGIFVDSA PRPLGNIIGG GAHASHATQF
160 170 180 190 200
QEFLVVPTGC ERVRDGIFAN AQVHKTVYDL LKKRGIRAGK GDEGAWAPPI
210 220 230 240 250
SDPEAFRILA EAISLVSDET GFEISPGIDA AASQLYDPES GMYRYRDGTV
260 270 280 290 300
RSPEDQVTYI ADLIDEFDLI YVEDPLYEED YEGFAALNRE AGNRCLICGD
310 320 330 340 350
DLFVTNTKRI QEGISHGSAN CVLIKPNQIG TLTDTHEAIH LAKRHGMETV
360 370 380 390 400
ISHRSGETED TTIAHLGCAF QCIFIKTGVV GGERTAKLNE LIRIEESIYE

RK
Length:402
Mass (Da):43,282
Last modified:March 21, 2006 - v1
Checksum:i3F08AA5C7816B356
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA. Translation: ABD42585.1.
RefSeqiWP_011449838.1. NC_007796.1.

Genome annotation databases

EnsemblBacteriaiABD42585; ABD42585; Mhun_2893.
GeneIDi3924453.
KEGGimhu:Mhun_2893.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO3_METHJ
AccessioniPrimary (citable) accession number: Q2FTN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 21, 2006
Last modified: June 7, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families