Q2FTH4 (DNLI_METHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	Mhun_2882

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864)

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 547	547	DNA ligase HAMAP MF_00407		PRO_0000365261
Sites
	Active site	246	1	N6-AMP-lysine intermediate By similarity
	Binding site	244	1	ATP By similarity
	Binding site	251	1	ATP By similarity
	Binding site	266	1	ATP By similarity
	Binding site	295	1	ATP By similarity
	Binding site	334	1	ATP By similarity
	Binding site	405	1	ATP By similarity
	Binding site	411	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FTH4 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: EEB45E15FAC1AA3F	FASTA	547	61,698
	10 20 30 40 50 60 MIFLEFAELC SRLEKISGRL ETISILAETI SSLSDDDLPH FCRLILGKPF PEWSGKKLGV 70 80 90 100 110 120 GPNLLYEAVA YVTGRKREEV IDRLSRVGDA GAAVEELLSQ KSQTSFFTVE LTLADIMAAL 130 140 150 160 170 180 IEISGMEGGR SQKEKVRVIQ RILSSASPLE GHYITAILLE DFRIGVGEGN LRDAIAQAFS 190 200 210 220 230 240 VDPNLVEYAN QVRNDMGEVA VLARKGEEAL RSVRLVPFHP VRMMLARQGT ISGVLKEGDP 250 260 270 280 290 300 VAVEFKYDGA RFQFHKQNKT CRMYSRRLEE VTNAMPDVVA LLDEALPDDI IVDGEVIAVQ 310 320 330 340 350 360 GGHPMPFQTV LRRFRRKHNV AEAADAITMI PNLFDILYYN QEMLIDLPFR ERRNILTQVA 370 380 390 400 410 420 SRYVTPQLVS DDETEIEAYY HTALDAGHEG VMLKLQGSRY TPGVRGKDWV KIKPEADTLD 430 440 450 460 470 480 LVVTGAEWGE GKRAHVFGSF LLSVRDDDRL VPISRVATGF SDEQLIWLFD TLQDDIIRKD 490 500 510 520 530 540 GKMVYFEPRL VFEIGYSEIQ KSPNYEGGYA LRFPRFIEVR EDKDLKEANT AEDVEERYIQ THSSLNT

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-1 / DSM 864.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000254 Genomic DNA. Translation: ABD42574.1.
RefSeq	YP_504293.1. NC_007796.1.
3D structure databases
HSSP	HSSP built from PDB template 1VS0 based on UniProtKB P71571.
ProteinModelPortal	Q2FTH4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FTH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3924177.
GenomeReviews	Gene locus Mhun_2882 in contig CP000254_GR.
KEGG	mhu:Mhun_2882.
NMPDR	fig|323259.5.peg.3020.
Phylogenomic databases
eggNOG	arNOG04596.
HOGENOM	HBG570821.
OMA	GRPRPFQ.
PhylomeDB	Q2FTH4.
ProtClustDB	CLSK949919.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_2882-MONOMER.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10747.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. False negative. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METHJ

Accession

Primary (citable) accession number: Q2FTH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 21, 2006
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program