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Q2FSY4

- Q2FSY4_METHJ

UniProt

Q2FSY4 - Q2FSY4_METHJ

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei138 – 1381Proton acceptorUniRule annotation
    Binding sitei140 – 1401SubstrateUniRule annotation
    Metal bindingi164 – 1641Magnesium; via carbamate groupUniRule annotation
    Metal bindingi166 – 1661MagnesiumUniRule annotation
    Metal bindingi167 – 1671MagnesiumUniRule annotation
    Active sitei255 – 2551Proton acceptorUniRule annotation
    Binding sitei256 – 2561SubstrateUniRule annotation
    Binding sitei288 – 2881SubstrateUniRule annotation
    Sitei295 – 2951Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: InterPro

    Keywords - Molecular functioni

    LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciMHUN323259:GH0L-2343-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    Ordered Locus Names:Mhun_2315Imported
    OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)Imported
    Taxonomic identifieri323259 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
    ProteomesiUP000001941: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei164 – 1641N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi323259.Mhun_2315.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2FSY4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni325 – 3273Substrate bindingUniRule annotation
    Regioni347 – 3504Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family.UniRule annotation
    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1850.
    HOGENOMiHOG000230832.
    KOiK01601.
    OMAiFTQDWAS.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProiIPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2FSY4-1 [UniParc]FASTAAdd to Basket

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    MTDVIATYYF RPREGVTPEW AAQAIAEEQT TGTWTDISTR QNYVHYLDGV    50
    VDEIHPSGGG YTCTIRYPSE IFEPGNIPQY LSVLAGNLFG LSRIAAVRLV 100
    DVEFSRDIVP FKGPKFGIEG VRKLAGTVDR PHVGTIIKPK VGLNPKDTAA 150
    VAYEAAIGGV DLIKDDETLT DQAFCPLGER LPLIMEQLDR VKSETGRNVL 200
    YAVNISSAGD KIVQRAREAA RMGANMLMID VIVCGFDAVR AVAEEPGINL 250
    PIHVHRTMHA AITRNPEHGI AMRPICRLVR MLGGDQLHTG TVSGKMEHDV 300
    TELRGDNLAL TEPFFDLKPT FPVASGGLHP GGVHKEVSML GRDIILQAGG 350
    GIHGHPDGTR VGATAMRQAV DAAVAGISPA TYAEDHPELK RALDKWGIA 399
    Length:399
    Mass (Da):43,121
    Last modified:March 21, 2006 - v1
    Checksum:i23E9553185B5A593
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000254 Genomic DNA. Translation: ABD42020.1.
    RefSeqiYP_503739.1. NC_007796.1.

    Genome annotation databases

    EnsemblBacteriaiABD42020; ABD42020; Mhun_2315.
    GeneIDi3922057.
    KEGGimhu:Mhun_2315.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000254 Genomic DNA. Translation: ABD42020.1 .
    RefSeqi YP_503739.1. NC_007796.1.

    3D structure databases

    ProteinModelPortali Q2FSY4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323259.Mhun_2315.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD42020 ; ABD42020 ; Mhun_2315 .
    GeneIDi 3922057.
    KEGGi mhu:Mhun_2315.

    Phylogenomic databases

    eggNOGi COG1850.
    HOGENOMi HOG000230832.
    KOi K01601.
    OMAi FTQDWAS.

    Enzyme and pathway databases

    BioCyci MHUN323259:GH0L-2343-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProi IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1Imported.

    Entry informationi

    Entry nameiQ2FSY4_METHJ
    AccessioniPrimary (citable) accession number: Q2FSY4
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 21, 2006
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3