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Q2FSY4

- Q2FSY4_METHJ

UniProt

Q2FSY4 - Q2FSY4_METHJ

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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381Proton acceptorUniRule annotation
Binding sitei140 – 1401SubstrateUniRule annotation
Metal bindingi164 – 1641Magnesium; via carbamate groupUniRule annotation
Metal bindingi166 – 1661MagnesiumUniRule annotation
Metal bindingi167 – 1671MagnesiumUniRule annotation
Active sitei255 – 2551Proton acceptorUniRule annotation
Binding sitei256 – 2561SubstrateUniRule annotation
Binding sitei288 – 2881SubstrateUniRule annotation
Sitei295 – 2951Transition state stabilizerUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. oxidoreductase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. AMP catabolic process Source: UniProtKB-HAMAP
  2. carbon fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciMHUN323259:GH0L-2343-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:Mhun_2315Imported
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)Imported
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
ProteomesiUP000001941: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei164 – 1641N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_2315.

Structurei

3D structure databases

ProteinModelPortaliQ2FSY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 3273Substrate bindingUniRule annotation
Regioni347 – 3504Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the RuBisCO large chain family.UniRule annotation
Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1850.
HOGENOMiHOG000230832.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
InterProiIPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2FSY4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDVIATYYF RPREGVTPEW AAQAIAEEQT TGTWTDISTR QNYVHYLDGV
60 70 80 90 100
VDEIHPSGGG YTCTIRYPSE IFEPGNIPQY LSVLAGNLFG LSRIAAVRLV
110 120 130 140 150
DVEFSRDIVP FKGPKFGIEG VRKLAGTVDR PHVGTIIKPK VGLNPKDTAA
160 170 180 190 200
VAYEAAIGGV DLIKDDETLT DQAFCPLGER LPLIMEQLDR VKSETGRNVL
210 220 230 240 250
YAVNISSAGD KIVQRAREAA RMGANMLMID VIVCGFDAVR AVAEEPGINL
260 270 280 290 300
PIHVHRTMHA AITRNPEHGI AMRPICRLVR MLGGDQLHTG TVSGKMEHDV
310 320 330 340 350
TELRGDNLAL TEPFFDLKPT FPVASGGLHP GGVHKEVSML GRDIILQAGG
360 370 380 390
GIHGHPDGTR VGATAMRQAV DAAVAGISPA TYAEDHPELK RALDKWGIA
Length:399
Mass (Da):43,121
Last modified:March 21, 2006 - v1
Checksum:i23E9553185B5A593
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000254 Genomic DNA. Translation: ABD42020.1.
RefSeqiWP_011449278.1. NC_007796.1.
YP_503739.1. NC_007796.1.

Genome annotation databases

EnsemblBacteriaiABD42020; ABD42020; Mhun_2315.
GeneIDi3922057.
KEGGimhu:Mhun_2315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000254 Genomic DNA. Translation: ABD42020.1 .
RefSeqi WP_011449278.1. NC_007796.1.
YP_503739.1. NC_007796.1.

3D structure databases

ProteinModelPortali Q2FSY4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 323259.Mhun_2315.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD42020 ; ABD42020 ; Mhun_2315 .
GeneIDi 3922057.
KEGGi mhu:Mhun_2315.

Phylogenomic databases

eggNOGi COG1850.
HOGENOMi HOG000230832.
KOi K01601.
OMAi FTQDWAS.

Enzyme and pathway databases

BioCyci MHUN323259:GH0L-2343-MONOMER.

Family and domain databases

Gene3Di 3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
InterProi IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view ]
Pfami PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1Imported.

Entry informationi

Entry nameiQ2FSY4_METHJ
AccessioniPrimary (citable) accession number: Q2FSY4
Entry historyi
Integrated into UniProtKB/TrEMBL: March 21, 2006
Last sequence update: March 21, 2006
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3