Q2FSK9 (ASPD_METHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase
EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	Mhun_2354

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864)

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000067305
Sites
	Active site	203	1	By similarity
	Binding site	119	1	NAD; via amide nitrogen By similarity
	Binding site	175	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FSK9 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: DB0A2E0F122E4710	FASTA	252	27,227
	10 20 30 40 50 60 MVRIGLLGCG NVGRIIATHQ DGFTVEALFD RLPDHAEELA RMCGAPAYAD FQEFISQDFD 70 80 90 100 110 120 ICVEAASVLA VREYAPKILE NGKHVLILSV GALSDTNFRK ILLDVARSQG KKIHIPSGAI 130 140 150 160 170 180 MGLDNLKVGG ISRIDSVLLR TTKSPASLGM QVSHRTLAFR GKANECIKQF PKNINVSVAL 190 200 210 220 230 240 ALAVHHDVDV ELWADPEVDR NIHDIFVSGE FGEASIRVVN HPSPDNPATS YLAALSVLSL 250 LKNLDSPLVI GS

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-1 / DSM 864.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000254 Genomic DNA. Translation: ABD42058.1.
RefSeq	YP_503777.1. NC_007796.1.
3D structure databases
HSSP	HSSP built from PDB template 1J5P based on UniProtKB Q9X1X6.
ProteinModelPortal	Q2FSK9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FSK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3923251.
GenomeReviews	Gene locus Mhun_2354 in contig CP000254_GR.
KEGG	mhu:Mhun_2354.
NMPDR	fig|323259.5.peg.2478.
Phylogenomic databases
eggNOG	arNOG05209.
HOGENOM	HBG649642.
OMA	ECAGHSA.
ProtClustDB	CLSK949789.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_2354-MONOMER.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR022487. Asp_DH_NAD_synth_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K06989.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMs	TIGR03855. NAD_NadX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_METHJ

Accession

Primary (citable) accession number: Q2FSK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 21, 2006
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program