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Protein

DNA polymerase II large subunit

Gene

polC

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Multifunctional enzyme, Nuclease, Nucleotidyltransferase, Transferase
Biological processDNA replication

Enzyme and pathway databases

BioCyciMHUN323259:G1G5Z-2577-MONOMER

Protein family/group databases

MEROPSiN10.006

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase II large subunitUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IIUniRule annotation
Alternative name(s):
Exodeoxyribonuclease large subunitUniRule annotation (EC:3.1.11.1UniRule annotation)
Cleaved into the following chain:
Alternative name(s):
Mhu pol II intein
Gene namesi
Name:polCUniRule annotation
Ordered Locus Names:Mhun_2435
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
Proteomesi
  • UP000001941 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002946941 – 872DNA polymerase II large subunit, 1st partSequence analysisAdd BLAST872
ChainiPRO_0000294695873 – 1037Mhu polC inteinSequence analysisAdd BLAST165
ChainiPRO_00002946961038 – 1286DNA polymerase II large subunit, 2nd partSequence analysisAdd BLAST249

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiQ2FSF9

Interactioni

Subunit structurei

Heterodimer of a large subunit and a small subunit.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_2435

Structurei

3D structure databases

ProteinModelPortaliQ2FSF9
SMRiQ2FSF9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal DNA polymerase II family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04447 Archaea
COG1933 LUCA
HOGENOMiHOG000286435
KOiK02322
OMAiKRRNCDG
OrthoDBiPOG093Z06CT

Family and domain databases

HAMAPiMF_00324 DNApol_II_L_arch, 1 hit
InterProiView protein in InterPro
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR030934 Intein_C
IPR006141 Intein_N
IPR004475 PolC_DP2
IPR016033 PolC_DP2_N
PANTHERiPTHR42210 PTHR42210, 1 hit
PfamiView protein in Pfam
PF03833 PolC_DP2, 1 hit
PIRSFiPIRSF016275 PolC_DP2, 1 hit
SMARTiView protein in SMART
SM00305 HintC, 1 hit
SM00306 HintN, 1 hit
SUPFAMiSSF51294 SSF51294, 1 hit
TIGRFAMsiTIGR01443 intein_Cterm, 1 hit
TIGR01445 intein_Nterm, 1 hit
TIGR00354 polC, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 1 hit
PS50817 INTEIN_N_TER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2FSF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADISPRMQE YYDRLNAGLA QAMEVAKTAR KTGLDPETVV EIPIANDLAD
60 70 80 90 100
RVEAQVGIPG VAQMIRELEA VMSREEASLH IGDYFAEKKF GETTKEEILD
110 120 130 140 150
HAIRTAMALL TEGVVSAPIE GIAKVAVKKN DDGSEYLAIY YAGPIRSAGG
160 170 180 190 200
TAQALSVLVG DYVRRILGMS RYMPREEEIE RYIEEIRQYN SIMSLQYLPS
210 220 230 240 250
EREIRLIISN CPVCIDGEPT EKEEVSGHRN LERVETNTVR GGMALVIAEG
260 270 280 290 300
LALKAPKVLK NVRKMKMDGW DWLEEMIQAT AGSSSSEEKE VGIHPKDKFL
310 320 330 340 350
RDLIGGRPVF SYPMREGGFR LVYGRSRNTG LAAAGLNPAT LHILGDFLAV
360 370 380 390 400
GTQMKIERPG KAAGISPVDS IQGPTVRLIN GDVVRIHDAK RARELSSQVS
410 420 430 440 450
HIIDVGEILI SYGEFMENNH VLVPSAYCES WWRLEGGTTR PADEDEAILQ
460 470 480 490 500
CESGLYLHPD YLYLWDDLKP AEIRSLAEFI HKHGTLADKT LILPLDPTIK
510 520 530 540 550
EYLERVLCEH QVREGMIRIT PCRIVLKCLG LDSSLALSDS WEKELPESAL
560 570 580 590 600
DLVQFLSGMK MRSKAGTRIG GRMGRPGKSK PREMKPAPHV LFPVGEAGGS
610 620 630 640 650
RRSVQEASKF SYQANTEGGN LQLEMGVRRC PACGTESYKN RCSCGTHTEP
660 670 680 690 700
VLSCSRCGIE VQGPVCPQCG MEPTSVRQYT VNVRQMLQQA FADLGMRDRD
710 720 730 740 750
VELVKGVKGL VSRNKPVELI EKGVIRAANK LFVFKDGTVR FDIIDMPLTH
760 770 780 790 800
FRPREIGVSV ERLRELGYLQ DIYGVPLQNP DQVLELPPQD ILVPEGCGDY
810 820 830 840 850
LFSCTRFIDT LLEKVYGLPP FYNLETRSDL VGHLVIGLAP HTSAGVLARI
860 870 880 890 900
IGFSKANVGY GHPFFHAAKR RNCFHGDTLI EIYADGILEE IPIRRFVLEH
910 920 930 940 950
LDLSQAGVDA LGTFYADPVR PAHVRSVDTG GIPHLRKITS VSVHKAPANL
960 970 980 990 1000
IQFSTSRGKN LLVTPDHAML VWDVSYLRKI RALEVKIGDA VPVWESGVVI
1010 1020 1030 1040 1050
SDRIVSIDYV PCEDERVYCL TVDRDHNVVG NGIFTGQCDG DEDCVMLLLD
1060 1070 1080 1090 1100
GLINFSRSFL PVTRGGSMDA PLVLTSRIDP TEIDKESHNL DVCATYPIEV
1110 1120 1130 1140 1150
YETALRYGNA KDVESLVDRV ERRLNTPAQI EGFFFTHDTS DISAGPLETM
1160 1170 1180 1190 1200
YTQLQSMLDK LDCELSLAKR IRAVDEHDVA ERVLKTHFIR DLQGNLSAFS
1210 1220 1230 1240 1250
KQKFRCTKCN TSYRRMPLAG KCKCGGNIIP TVHEGSVKKY LQMSRQICEE
1260 1270 1280
YNITEYTRQR IEVIDMNIES TFGEEKVEQM GLADFM
Length:1,286
Mass (Da):143,304
Last modified:March 21, 2006 - v1
Checksum:i7169201A09413EB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA Translation: ABD42136.1
RefSeqiWP_011449394.1, NC_007796.1

Genome annotation databases

EnsemblBacteriaiABD42136; ABD42136; Mhun_2435
GeneIDi3922423
KEGGimhu:Mhun_2435

Similar proteinsi

Entry informationi

Entry nameiDP2L_METHJ
AccessioniPrimary (citable) accession number: Q2FSF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 21, 2006
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

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