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Q2FSF9 (DP2L_METHJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase II large subunit

Short name=Pol II
EC=2.7.7.7

Cleaved into the following chain:

  1. Mhu polC intein
    Alternative name(s):
    Mhu pol II intein
Gene names
Name:polC
Ordered Locus Names:Mhun_2435
OrganismMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1) [Reference proteome] [HAMAP]
Taxonomic identifier323259 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum

Protein attributes

Sequence length1286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP-Rule MF_00324

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP-Rule MF_00324

Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP-Rule MF_00324

Subunit structure

Heterodimer of a large subunit and a small subunit By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00324

Sequence similarities

Belongs to the archaeal DNA polymerase II family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 872872DNA polymerase II large subunit, 1st part Potential
PRO_0000294694
Chain873 – 1037165Mhu polC intein Potential
PRO_0000294695
Chain1038 – 1286249DNA polymerase II large subunit, 2nd part Potential
PRO_0000294696

Sequences

Sequence LengthMass (Da)Tools
Q2FSF9 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 7169201A09413EB2

FASTA1,286143,304
        10         20         30         40         50         60 
MADISPRMQE YYDRLNAGLA QAMEVAKTAR KTGLDPETVV EIPIANDLAD RVEAQVGIPG 

        70         80         90        100        110        120 
VAQMIRELEA VMSREEASLH IGDYFAEKKF GETTKEEILD HAIRTAMALL TEGVVSAPIE 

       130        140        150        160        170        180 
GIAKVAVKKN DDGSEYLAIY YAGPIRSAGG TAQALSVLVG DYVRRILGMS RYMPREEEIE 

       190        200        210        220        230        240 
RYIEEIRQYN SIMSLQYLPS EREIRLIISN CPVCIDGEPT EKEEVSGHRN LERVETNTVR 

       250        260        270        280        290        300 
GGMALVIAEG LALKAPKVLK NVRKMKMDGW DWLEEMIQAT AGSSSSEEKE VGIHPKDKFL 

       310        320        330        340        350        360 
RDLIGGRPVF SYPMREGGFR LVYGRSRNTG LAAAGLNPAT LHILGDFLAV GTQMKIERPG 

       370        380        390        400        410        420 
KAAGISPVDS IQGPTVRLIN GDVVRIHDAK RARELSSQVS HIIDVGEILI SYGEFMENNH 

       430        440        450        460        470        480 
VLVPSAYCES WWRLEGGTTR PADEDEAILQ CESGLYLHPD YLYLWDDLKP AEIRSLAEFI 

       490        500        510        520        530        540 
HKHGTLADKT LILPLDPTIK EYLERVLCEH QVREGMIRIT PCRIVLKCLG LDSSLALSDS 

       550        560        570        580        590        600 
WEKELPESAL DLVQFLSGMK MRSKAGTRIG GRMGRPGKSK PREMKPAPHV LFPVGEAGGS 

       610        620        630        640        650        660 
RRSVQEASKF SYQANTEGGN LQLEMGVRRC PACGTESYKN RCSCGTHTEP VLSCSRCGIE 

       670        680        690        700        710        720 
VQGPVCPQCG MEPTSVRQYT VNVRQMLQQA FADLGMRDRD VELVKGVKGL VSRNKPVELI 

       730        740        750        760        770        780 
EKGVIRAANK LFVFKDGTVR FDIIDMPLTH FRPREIGVSV ERLRELGYLQ DIYGVPLQNP 

       790        800        810        820        830        840 
DQVLELPPQD ILVPEGCGDY LFSCTRFIDT LLEKVYGLPP FYNLETRSDL VGHLVIGLAP 

       850        860        870        880        890        900 
HTSAGVLARI IGFSKANVGY GHPFFHAAKR RNCFHGDTLI EIYADGILEE IPIRRFVLEH 

       910        920        930        940        950        960 
LDLSQAGVDA LGTFYADPVR PAHVRSVDTG GIPHLRKITS VSVHKAPANL IQFSTSRGKN 

       970        980        990       1000       1010       1020 
LLVTPDHAML VWDVSYLRKI RALEVKIGDA VPVWESGVVI SDRIVSIDYV PCEDERVYCL 

      1030       1040       1050       1060       1070       1080 
TVDRDHNVVG NGIFTGQCDG DEDCVMLLLD GLINFSRSFL PVTRGGSMDA PLVLTSRIDP 

      1090       1100       1110       1120       1130       1140 
TEIDKESHNL DVCATYPIEV YETALRYGNA KDVESLVDRV ERRLNTPAQI EGFFFTHDTS 

      1150       1160       1170       1180       1190       1200 
DISAGPLETM YTQLQSMLDK LDCELSLAKR IRAVDEHDVA ERVLKTHFIR DLQGNLSAFS 

      1210       1220       1230       1240       1250       1260 
KQKFRCTKCN TSYRRMPLAG KCKCGGNIIP TVHEGSVKKY LQMSRQICEE YNITEYTRQR 

      1270       1280 
IEVIDMNIES TFGEEKVEQM GLADFM 

« Hide

References

[1]"Complete sequence of Methanospirillum hungatei JF-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000254 Genomic DNA. Translation: ABD42136.1.
RefSeqYP_503855.1. NC_007796.1.

3D structure databases

ProteinModelPortalQ2FSF9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323259.Mhun_2435.

Protein family/group databases

MEROPSN10.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD42136; ABD42136; Mhun_2435.
GeneID3922423.
KEGGmhu:Mhun_2435.

Phylogenomic databases

eggNOGCOG1933.
HOGENOMHOG000286435.
KOK02322.
OMARNCDGDE.
ProtClustDBPRK14714.

Enzyme and pathway databases

BioCycMHUN323259:GH0L-2468-MONOMER.

Family and domain databases

HAMAPMF_00324. DNApol_II_L_arch.
InterProIPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamPF03833. PolC_DP2. 1 hit.
[Graphical view]
PIRSFPIRSF016275. PolC_DP2. 1 hit.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDP2L_METHJ
AccessionPrimary (citable) accession number: Q2FSF9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 21, 2006
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries