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Q2FSF9

- DP2L_METHJ

UniProt

Q2FSF9 - DP2L_METHJ

Protein

DNA polymerase II large subunit

Gene

polC

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation
    Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.UniRule annotation

    GO - Molecular functioni

    1. 3'-5' exonuclease activity Source: UniProtKB-HAMAP
    2. DNA binding Source: UniProtKB-HAMAP
    3. DNA-directed DNA polymerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA catabolic process, exonucleolytic Source: UniProtKB-HAMAP
    2. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciMHUN323259:GH0L-2468-MONOMER.

    Protein family/group databases

    MEROPSiN10.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase II large subunitUniRule annotation (EC:2.7.7.7UniRule annotation)
    Short name:
    Pol IIUniRule annotation
    Cleaved into the following chain:
    Alternative name(s):
    Mhu pol II intein
    Gene namesi
    Name:polCUniRule annotation
    Ordered Locus Names:Mhun_2435
    OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
    Taxonomic identifieri323259 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
    ProteomesiUP000001941: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 872872DNA polymerase II large subunit, 1st partSequence AnalysisPRO_0000294694Add
    BLAST
    Chaini873 – 1037165Mhu polC inteinSequence AnalysisPRO_0000294695Add
    BLAST
    Chaini1038 – 1286249DNA polymerase II large subunit, 2nd partSequence AnalysisPRO_0000294696Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Heterodimer of a large subunit and a small subunit.UniRule annotation

    Protein-protein interaction databases

    STRINGi323259.Mhun_2435.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2FSF9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the archaeal DNA polymerase II family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1933.
    HOGENOMiHOG000286435.
    KOiK02322.
    OMAiRNCDGDE.

    Family and domain databases

    Gene3Di2.170.16.10. 1 hit.
    HAMAPiMF_00324. DNApol_II_L_arch.
    InterProiIPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR006141. Intein_splice_site.
    IPR004475. PolC_DP2.
    IPR016033. PolC_DP2_N.
    [Graphical view]
    PfamiPF03833. PolC_DP2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016275. PolC_DP2. 1 hit.
    SMARTiSM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 1 hit.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00354. polC. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2FSF9-1 [UniParc]FASTAAdd to Basket

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    MADISPRMQE YYDRLNAGLA QAMEVAKTAR KTGLDPETVV EIPIANDLAD     50
    RVEAQVGIPG VAQMIRELEA VMSREEASLH IGDYFAEKKF GETTKEEILD 100
    HAIRTAMALL TEGVVSAPIE GIAKVAVKKN DDGSEYLAIY YAGPIRSAGG 150
    TAQALSVLVG DYVRRILGMS RYMPREEEIE RYIEEIRQYN SIMSLQYLPS 200
    EREIRLIISN CPVCIDGEPT EKEEVSGHRN LERVETNTVR GGMALVIAEG 250
    LALKAPKVLK NVRKMKMDGW DWLEEMIQAT AGSSSSEEKE VGIHPKDKFL 300
    RDLIGGRPVF SYPMREGGFR LVYGRSRNTG LAAAGLNPAT LHILGDFLAV 350
    GTQMKIERPG KAAGISPVDS IQGPTVRLIN GDVVRIHDAK RARELSSQVS 400
    HIIDVGEILI SYGEFMENNH VLVPSAYCES WWRLEGGTTR PADEDEAILQ 450
    CESGLYLHPD YLYLWDDLKP AEIRSLAEFI HKHGTLADKT LILPLDPTIK 500
    EYLERVLCEH QVREGMIRIT PCRIVLKCLG LDSSLALSDS WEKELPESAL 550
    DLVQFLSGMK MRSKAGTRIG GRMGRPGKSK PREMKPAPHV LFPVGEAGGS 600
    RRSVQEASKF SYQANTEGGN LQLEMGVRRC PACGTESYKN RCSCGTHTEP 650
    VLSCSRCGIE VQGPVCPQCG MEPTSVRQYT VNVRQMLQQA FADLGMRDRD 700
    VELVKGVKGL VSRNKPVELI EKGVIRAANK LFVFKDGTVR FDIIDMPLTH 750
    FRPREIGVSV ERLRELGYLQ DIYGVPLQNP DQVLELPPQD ILVPEGCGDY 800
    LFSCTRFIDT LLEKVYGLPP FYNLETRSDL VGHLVIGLAP HTSAGVLARI 850
    IGFSKANVGY GHPFFHAAKR RNCFHGDTLI EIYADGILEE IPIRRFVLEH 900
    LDLSQAGVDA LGTFYADPVR PAHVRSVDTG GIPHLRKITS VSVHKAPANL 950
    IQFSTSRGKN LLVTPDHAML VWDVSYLRKI RALEVKIGDA VPVWESGVVI 1000
    SDRIVSIDYV PCEDERVYCL TVDRDHNVVG NGIFTGQCDG DEDCVMLLLD 1050
    GLINFSRSFL PVTRGGSMDA PLVLTSRIDP TEIDKESHNL DVCATYPIEV 1100
    YETALRYGNA KDVESLVDRV ERRLNTPAQI EGFFFTHDTS DISAGPLETM 1150
    YTQLQSMLDK LDCELSLAKR IRAVDEHDVA ERVLKTHFIR DLQGNLSAFS 1200
    KQKFRCTKCN TSYRRMPLAG KCKCGGNIIP TVHEGSVKKY LQMSRQICEE 1250
    YNITEYTRQR IEVIDMNIES TFGEEKVEQM GLADFM 1286
    Length:1,286
    Mass (Da):143,304
    Last modified:March 21, 2006 - v1
    Checksum:i7169201A09413EB2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000254 Genomic DNA. Translation: ABD42136.1.
    RefSeqiYP_503855.1. NC_007796.1.

    Genome annotation databases

    EnsemblBacteriaiABD42136; ABD42136; Mhun_2435.
    GeneIDi3922423.
    KEGGimhu:Mhun_2435.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000254 Genomic DNA. Translation: ABD42136.1 .
    RefSeqi YP_503855.1. NC_007796.1.

    3D structure databases

    ProteinModelPortali Q2FSF9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323259.Mhun_2435.

    Protein family/group databases

    MEROPSi N10.006.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD42136 ; ABD42136 ; Mhun_2435 .
    GeneIDi 3922423.
    KEGGi mhu:Mhun_2435.

    Phylogenomic databases

    eggNOGi COG1933.
    HOGENOMi HOG000286435.
    KOi K02322.
    OMAi RNCDGDE.

    Enzyme and pathway databases

    BioCyci MHUN323259:GH0L-2468-MONOMER.

    Family and domain databases

    Gene3Di 2.170.16.10. 1 hit.
    HAMAPi MF_00324. DNApol_II_L_arch.
    InterProi IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR006141. Intein_splice_site.
    IPR004475. PolC_DP2.
    IPR016033. PolC_DP2_N.
    [Graphical view ]
    Pfami PF03833. PolC_DP2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016275. PolC_DP2. 1 hit.
    SMARTi SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 1 hit.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00354. polC. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

    Entry informationi

    Entry nameiDP2L_METHJ
    AccessioniPrimary (citable) accession number: Q2FSF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3