Q2FSF9 (DP2L_METHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Cleaved into the following chain:

1. Mhu polC intein
   Alternative name(s):
   Mhu pol II intein

Gene names

Name:	polC
Ordered Locus Names:	Mhun_2435

Organism

Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1) [Reference proteome] [HAMAP]

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum › Methanospirillum hungatei

Protein attributes

Sequence length	1286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP-Rule MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP-Rule MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP-Rule MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular_function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 872	872	DNA polymerase II large subunit, 1st part Potential		PRO_0000294694
	Chain	873 – 1037	165	Mhu polC intein Potential		PRO_0000294695
	Chain	1038 – 1286	249	DNA polymerase II large subunit, 2nd part Potential		PRO_0000294696

Sequences

	Sequence		Length	Mass (Da)
	Q2FSF9 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 7169201A09413EB2	FASTA	1,286	143,304
	10 20 30 40 50 60 MADISPRMQE YYDRLNAGLA QAMEVAKTAR KTGLDPETVV EIPIANDLAD RVEAQVGIPG 70 80 90 100 110 120 VAQMIRELEA VMSREEASLH IGDYFAEKKF GETTKEEILD HAIRTAMALL TEGVVSAPIE 130 140 150 160 170 180 GIAKVAVKKN DDGSEYLAIY YAGPIRSAGG TAQALSVLVG DYVRRILGMS RYMPREEEIE 190 200 210 220 230 240 RYIEEIRQYN SIMSLQYLPS EREIRLIISN CPVCIDGEPT EKEEVSGHRN LERVETNTVR 250 260 270 280 290 300 GGMALVIAEG LALKAPKVLK NVRKMKMDGW DWLEEMIQAT AGSSSSEEKE VGIHPKDKFL 310 320 330 340 350 360 RDLIGGRPVF SYPMREGGFR LVYGRSRNTG LAAAGLNPAT LHILGDFLAV GTQMKIERPG 370 380 390 400 410 420 KAAGISPVDS IQGPTVRLIN GDVVRIHDAK RARELSSQVS HIIDVGEILI SYGEFMENNH 430 440 450 460 470 480 VLVPSAYCES WWRLEGGTTR PADEDEAILQ CESGLYLHPD YLYLWDDLKP AEIRSLAEFI 490 500 510 520 530 540 HKHGTLADKT LILPLDPTIK EYLERVLCEH QVREGMIRIT PCRIVLKCLG LDSSLALSDS 550 560 570 580 590 600 WEKELPESAL DLVQFLSGMK MRSKAGTRIG GRMGRPGKSK PREMKPAPHV LFPVGEAGGS 610 620 630 640 650 660 RRSVQEASKF SYQANTEGGN LQLEMGVRRC PACGTESYKN RCSCGTHTEP VLSCSRCGIE 670 680 690 700 710 720 VQGPVCPQCG MEPTSVRQYT VNVRQMLQQA FADLGMRDRD VELVKGVKGL VSRNKPVELI 730 740 750 760 770 780 EKGVIRAANK LFVFKDGTVR FDIIDMPLTH FRPREIGVSV ERLRELGYLQ DIYGVPLQNP 790 800 810 820 830 840 DQVLELPPQD ILVPEGCGDY LFSCTRFIDT LLEKVYGLPP FYNLETRSDL VGHLVIGLAP 850 860 870 880 890 900 HTSAGVLARI IGFSKANVGY GHPFFHAAKR RNCFHGDTLI EIYADGILEE IPIRRFVLEH 910 920 930 940 950 960 LDLSQAGVDA LGTFYADPVR PAHVRSVDTG GIPHLRKITS VSVHKAPANL IQFSTSRGKN 970 980 990 1000 1010 1020 LLVTPDHAML VWDVSYLRKI RALEVKIGDA VPVWESGVVI SDRIVSIDYV PCEDERVYCL 1030 1040 1050 1060 1070 1080 TVDRDHNVVG NGIFTGQCDG DEDCVMLLLD GLINFSRSFL PVTRGGSMDA PLVLTSRIDP 1090 1100 1110 1120 1130 1140 TEIDKESHNL DVCATYPIEV YETALRYGNA KDVESLVDRV ERRLNTPAQI EGFFFTHDTS 1150 1160 1170 1180 1190 1200 DISAGPLETM YTQLQSMLDK LDCELSLAKR IRAVDEHDVA ERVLKTHFIR DLQGNLSAFS 1210 1220 1230 1240 1250 1260 KQKFRCTKCN TSYRRMPLAG KCKCGGNIIP TVHEGSVKKY LQMSRQICEE YNITEYTRQR 1270 1280 IEVIDMNIES TFGEEKVEQM GLADFM

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000254 Genomic DNA. Translation: ABD42136.1.
RefSeq	YP_503855.1. NC_007796.1.
3D structure databases
ProteinModelPortal	Q2FSF9.
ModBase	Search...
Protein-protein interaction databases
STRING	323259.Mhun_2435.
Protein family/group databases
MEROPS	N10.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD42136; ABD42136; Mhun_2435.
GeneID	3922423.
KEGG	mhu:Mhun_2435.
Phylogenomic databases
eggNOG	COG1933.
HOGENOM	HOG000286435.
KO	K02322.
OMA	RNCDGDE.
ProtClustDB	PRK14714.
Enzyme and pathway databases
BioCyc	MHUN323259:GH0L-2468-MONOMER.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch.
InterPro	IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006141. Intein_splice_site. IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR00354. polC. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DP2L_METHJ

Accession

Primary (citable) accession number: Q2FSF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 21, 2006
Last modified:	May 29, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program