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Q2FSF9

- DP2L_METHJ

UniProt

Q2FSF9 - DP2L_METHJ

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Protein
DNA polymerase II large subunit
Gene
polC, Mhun_2435
Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation
Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.UniRule annotation

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA-directed DNA polymerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA catabolic process, exonucleolytic Source: UniProtKB-HAMAP
  2. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMHUN323259:GH0L-2468-MONOMER.

Protein family/group databases

MEROPSiN10.006.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase II large subunit (EC:2.7.7.7)
Short name:
Pol II
Cleaved into the following chain:
Alternative name(s):
Mhu pol II intein
Gene namesi
Name:polC
Ordered Locus Names:Mhun_2435
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
ProteomesiUP000001941: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 872872DNA polymerase II large subunit, 1st part Reviewed prediction
PRO_0000294694Add
BLAST
Chaini873 – 1037165Mhu polC intein Reviewed prediction
PRO_0000294695Add
BLAST
Chaini1038 – 1286249DNA polymerase II large subunit, 2nd part Reviewed prediction
PRO_0000294696Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heterodimer of a large subunit and a small subunit By similarity.

Protein-protein interaction databases

STRINGi323259.Mhun_2435.

Structurei

3D structure databases

ProteinModelPortaliQ2FSF9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1933.
HOGENOMiHOG000286435.
KOiK02322.
OMAiRNCDGDE.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
HAMAPiMF_00324. DNApol_II_L_arch.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamiPF03833. PolC_DP2. 1 hit.
[Graphical view]
PIRSFiPIRSF016275. PolC_DP2. 1 hit.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2FSF9-1 [UniParc]FASTAAdd to Basket

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MADISPRMQE YYDRLNAGLA QAMEVAKTAR KTGLDPETVV EIPIANDLAD     50
RVEAQVGIPG VAQMIRELEA VMSREEASLH IGDYFAEKKF GETTKEEILD 100
HAIRTAMALL TEGVVSAPIE GIAKVAVKKN DDGSEYLAIY YAGPIRSAGG 150
TAQALSVLVG DYVRRILGMS RYMPREEEIE RYIEEIRQYN SIMSLQYLPS 200
EREIRLIISN CPVCIDGEPT EKEEVSGHRN LERVETNTVR GGMALVIAEG 250
LALKAPKVLK NVRKMKMDGW DWLEEMIQAT AGSSSSEEKE VGIHPKDKFL 300
RDLIGGRPVF SYPMREGGFR LVYGRSRNTG LAAAGLNPAT LHILGDFLAV 350
GTQMKIERPG KAAGISPVDS IQGPTVRLIN GDVVRIHDAK RARELSSQVS 400
HIIDVGEILI SYGEFMENNH VLVPSAYCES WWRLEGGTTR PADEDEAILQ 450
CESGLYLHPD YLYLWDDLKP AEIRSLAEFI HKHGTLADKT LILPLDPTIK 500
EYLERVLCEH QVREGMIRIT PCRIVLKCLG LDSSLALSDS WEKELPESAL 550
DLVQFLSGMK MRSKAGTRIG GRMGRPGKSK PREMKPAPHV LFPVGEAGGS 600
RRSVQEASKF SYQANTEGGN LQLEMGVRRC PACGTESYKN RCSCGTHTEP 650
VLSCSRCGIE VQGPVCPQCG MEPTSVRQYT VNVRQMLQQA FADLGMRDRD 700
VELVKGVKGL VSRNKPVELI EKGVIRAANK LFVFKDGTVR FDIIDMPLTH 750
FRPREIGVSV ERLRELGYLQ DIYGVPLQNP DQVLELPPQD ILVPEGCGDY 800
LFSCTRFIDT LLEKVYGLPP FYNLETRSDL VGHLVIGLAP HTSAGVLARI 850
IGFSKANVGY GHPFFHAAKR RNCFHGDTLI EIYADGILEE IPIRRFVLEH 900
LDLSQAGVDA LGTFYADPVR PAHVRSVDTG GIPHLRKITS VSVHKAPANL 950
IQFSTSRGKN LLVTPDHAML VWDVSYLRKI RALEVKIGDA VPVWESGVVI 1000
SDRIVSIDYV PCEDERVYCL TVDRDHNVVG NGIFTGQCDG DEDCVMLLLD 1050
GLINFSRSFL PVTRGGSMDA PLVLTSRIDP TEIDKESHNL DVCATYPIEV 1100
YETALRYGNA KDVESLVDRV ERRLNTPAQI EGFFFTHDTS DISAGPLETM 1150
YTQLQSMLDK LDCELSLAKR IRAVDEHDVA ERVLKTHFIR DLQGNLSAFS 1200
KQKFRCTKCN TSYRRMPLAG KCKCGGNIIP TVHEGSVKKY LQMSRQICEE 1250
YNITEYTRQR IEVIDMNIES TFGEEKVEQM GLADFM 1286
Length:1,286
Mass (Da):143,304
Last modified:March 21, 2006 - v1
Checksum:i7169201A09413EB2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000254 Genomic DNA. Translation: ABD42136.1.
RefSeqiYP_503855.1. NC_007796.1.

Genome annotation databases

EnsemblBacteriaiABD42136; ABD42136; Mhun_2435.
GeneIDi3922423.
KEGGimhu:Mhun_2435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000254 Genomic DNA. Translation: ABD42136.1 .
RefSeqi YP_503855.1. NC_007796.1.

3D structure databases

ProteinModelPortali Q2FSF9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 323259.Mhun_2435.

Protein family/group databases

MEROPSi N10.006.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD42136 ; ABD42136 ; Mhun_2435 .
GeneIDi 3922423.
KEGGi mhu:Mhun_2435.

Phylogenomic databases

eggNOGi COG1933.
HOGENOMi HOG000286435.
KOi K02322.
OMAi RNCDGDE.

Enzyme and pathway databases

BioCyci MHUN323259:GH0L-2468-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 1 hit.
HAMAPi MF_00324. DNApol_II_L_arch.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view ]
Pfami PF03833. PolC_DP2. 1 hit.
[Graphical view ]
PIRSFi PIRSF016275. PolC_DP2. 1 hit.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

Entry informationi

Entry nameiDP2L_METHJ
AccessioniPrimary (citable) accession number: Q2FSF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 21, 2006
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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