Reviewed,
UniProtKB/Swiss-Prot Q2FSF9 (DP2L_METHJ)
Last modified
May 5, 2009.
Version 20.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: DNA polymerase II large subunit Short name=Pol II EC=2.7.7.7 Cleaved into the following chain: 1- Recommended name: Mhu polC intein Alternative name(s): Mhu pol II intein | ||||
| Gene names |
| ||||
| Organism | Methanospirillum hungatei (strain JF-1 / DSM 864) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 323259 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum |
Protein attributes
| Sequence length | 1286 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. |
| Catalytic activity | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324 |
| Subunit structure | Heterodimer of a large subunit and a small subunit By similarity. |
| Post-translational modification | This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. |
| Sequence similarities | Belongs to the archaeal DNA polymerase II family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA replication |
| Ligand | DNA-binding |
| Molecular function | DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase |
| PTM | Autocatalytic cleavage Protein splicing |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replicationInferred from electronic annotation. Source: HAMAP intein-mediated protein splicingInferred from electronic annotation. Source: InterPro |
| Molecular function | 3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA bindingInferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 872 | 872 | DNA polymerase II large subunit, 1st part Potential | PRO_0000294694 | |||||
| Chain | 873 – 1037 | 165 | Mhu polC intein Potential | PRO_0000294695 | |||||
| Chain | 1038 – 1286 | 249 | DNA polymerase II large subunit, 2nd part Potential | PRO_0000294696 | |||||
Sequences
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References
| [1] | "Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. Richardson P.Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| CP000254 Genomic DNA. Translation: ABD42136.1. | |
| RefSeq | YP_503855.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 3922423. |
| GenomeReviews | Gene locus Mhun_2435 in contig CP000254_GR. |
| KEGG | mhu:Mhun_2435. |
| NMPDR | fig|323259.5.peg.2561. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q2FSF9. |
| OMA | Q2FSF9. NCDGDED. |
Enzyme and pathway databases | |
| BioCyc | MHUN323259:MHUN_2435-MON. |
Family and domain databases | |
| HAMAP | MF_00324. [Tree] |
| InterPro | IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006141. Intein_splicing_site. IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view] |
| Pfam | PF03833. PolC_DP2. 1 hit. [Graphical view] |
| PIRSF | PIRSF016275. PolC_DP2. 1 hit. |
| SMART | SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01443. intein_Cterm. 1 hit. TIGR00354. polC. 1 hit. |
| PROSITE | PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DP2L_METHJ | ||||||||
| Accession | Primary (citable) accession number: Q2FSF9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Intein-containing proteins List of intein-containing protein entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


