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Protein

DNA polymerase II large subunit

Gene

polC

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation
Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.UniRule annotation

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA-directed DNA polymerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA catabolic process, exonucleolytic Source: UniProtKB-HAMAP
  2. DNA-dependent DNA replication Source: UniProtKB-HAMAP
  3. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMHUN323259:GH0L-2468-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase II large subunitUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IIUniRule annotation
Cleaved into the following chain:
Alternative name(s):
Mhu pol II intein
Gene namesi
Name:polCUniRule annotation
Ordered Locus Names:Mhun_2435
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
ProteomesiUP000001941 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 872872DNA polymerase II large subunit, 1st partSequence AnalysisPRO_0000294694Add
BLAST
Chaini873 – 1037165Mhu polC inteinSequence AnalysisPRO_0000294695Add
BLAST
Chaini1038 – 1286249DNA polymerase II large subunit, 2nd partSequence AnalysisPRO_0000294696Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heterodimer of a large subunit and a small subunit.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_2435.

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal DNA polymerase II family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1933.
HOGENOMiHOG000286435.
KOiK02322.
OMAiRNCDGDE.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
HAMAPiMF_00324. DNApol_II_L_arch.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamiPF03833. PolC_DP2. 1 hit.
[Graphical view]
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2FSF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADISPRMQE YYDRLNAGLA QAMEVAKTAR KTGLDPETVV EIPIANDLAD
60 70 80 90 100
RVEAQVGIPG VAQMIRELEA VMSREEASLH IGDYFAEKKF GETTKEEILD
110 120 130 140 150
HAIRTAMALL TEGVVSAPIE GIAKVAVKKN DDGSEYLAIY YAGPIRSAGG
160 170 180 190 200
TAQALSVLVG DYVRRILGMS RYMPREEEIE RYIEEIRQYN SIMSLQYLPS
210 220 230 240 250
EREIRLIISN CPVCIDGEPT EKEEVSGHRN LERVETNTVR GGMALVIAEG
260 270 280 290 300
LALKAPKVLK NVRKMKMDGW DWLEEMIQAT AGSSSSEEKE VGIHPKDKFL
310 320 330 340 350
RDLIGGRPVF SYPMREGGFR LVYGRSRNTG LAAAGLNPAT LHILGDFLAV
360 370 380 390 400
GTQMKIERPG KAAGISPVDS IQGPTVRLIN GDVVRIHDAK RARELSSQVS
410 420 430 440 450
HIIDVGEILI SYGEFMENNH VLVPSAYCES WWRLEGGTTR PADEDEAILQ
460 470 480 490 500
CESGLYLHPD YLYLWDDLKP AEIRSLAEFI HKHGTLADKT LILPLDPTIK
510 520 530 540 550
EYLERVLCEH QVREGMIRIT PCRIVLKCLG LDSSLALSDS WEKELPESAL
560 570 580 590 600
DLVQFLSGMK MRSKAGTRIG GRMGRPGKSK PREMKPAPHV LFPVGEAGGS
610 620 630 640 650
RRSVQEASKF SYQANTEGGN LQLEMGVRRC PACGTESYKN RCSCGTHTEP
660 670 680 690 700
VLSCSRCGIE VQGPVCPQCG MEPTSVRQYT VNVRQMLQQA FADLGMRDRD
710 720 730 740 750
VELVKGVKGL VSRNKPVELI EKGVIRAANK LFVFKDGTVR FDIIDMPLTH
760 770 780 790 800
FRPREIGVSV ERLRELGYLQ DIYGVPLQNP DQVLELPPQD ILVPEGCGDY
810 820 830 840 850
LFSCTRFIDT LLEKVYGLPP FYNLETRSDL VGHLVIGLAP HTSAGVLARI
860 870 880 890 900
IGFSKANVGY GHPFFHAAKR RNCFHGDTLI EIYADGILEE IPIRRFVLEH
910 920 930 940 950
LDLSQAGVDA LGTFYADPVR PAHVRSVDTG GIPHLRKITS VSVHKAPANL
960 970 980 990 1000
IQFSTSRGKN LLVTPDHAML VWDVSYLRKI RALEVKIGDA VPVWESGVVI
1010 1020 1030 1040 1050
SDRIVSIDYV PCEDERVYCL TVDRDHNVVG NGIFTGQCDG DEDCVMLLLD
1060 1070 1080 1090 1100
GLINFSRSFL PVTRGGSMDA PLVLTSRIDP TEIDKESHNL DVCATYPIEV
1110 1120 1130 1140 1150
YETALRYGNA KDVESLVDRV ERRLNTPAQI EGFFFTHDTS DISAGPLETM
1160 1170 1180 1190 1200
YTQLQSMLDK LDCELSLAKR IRAVDEHDVA ERVLKTHFIR DLQGNLSAFS
1210 1220 1230 1240 1250
KQKFRCTKCN TSYRRMPLAG KCKCGGNIIP TVHEGSVKKY LQMSRQICEE
1260 1270 1280
YNITEYTRQR IEVIDMNIES TFGEEKVEQM GLADFM
Length:1,286
Mass (Da):143,304
Last modified:March 21, 2006 - v1
Checksum:i7169201A09413EB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA. Translation: ABD42136.1.
RefSeqiYP_503855.1. NC_007796.1.

Genome annotation databases

EnsemblBacteriaiABD42136; ABD42136; Mhun_2435.
GeneIDi3922423.
KEGGimhu:Mhun_2435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA. Translation: ABD42136.1.
RefSeqiYP_503855.1. NC_007796.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323259.Mhun_2435.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD42136; ABD42136; Mhun_2435.
GeneIDi3922423.
KEGGimhu:Mhun_2435.

Phylogenomic databases

eggNOGiCOG1933.
HOGENOMiHOG000286435.
KOiK02322.
OMAiRNCDGDE.

Enzyme and pathway databases

BioCyciMHUN323259:GH0L-2468-MONOMER.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
HAMAPiMF_00324. DNApol_II_L_arch.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamiPF03833. PolC_DP2. 1 hit.
[Graphical view]
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

Entry informationi

Entry nameiDP2L_METHJ
AccessioniPrimary (citable) accession number: Q2FSF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 21, 2006
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.