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Protein

7-carboxy-7-deazaguanine synthase

Gene

queE

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.UniRule annotation

Catalytic activityi

6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH3.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation
  • Mg2+UniRule annotation

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57SubstrateUniRule annotation1
Metal bindingi61Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi65Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi68Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi70MagnesiumUniRule annotation1
Binding sitei100SubstrateUniRule annotation1
Binding sitei102S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei234Substrate; via carboxylateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionLyase
Ligand4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00391

Names & Taxonomyi

Protein namesi
Recommended name:
7-carboxy-7-deazaguanine synthaseUniRule annotation (EC:4.3.99.3UniRule annotation)
Short name:
CDG synthaseUniRule annotation
Alternative name(s):
Archaeosine biosynthesis protein QueEUniRule annotation
Gene namesi
Name:queEUniRule annotation
Ordered Locus Names:Mhun_2832
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
Proteomesi
  • UP000001941 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004162201 – 2347-carboxy-7-deazaguanine synthaseAdd BLAST234

Proteomic databases

PRIDEiQ2FS67

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_2832

Structurei

3D structure databases

ProteinModelPortaliQ2FS67
SMRiQ2FS67
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 44Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02173 Archaea
COG0602 LUCA
HOGENOMiHOG000266145
KOiK10026
OMAiCDTEYAF
OrthoDBiPOG093Z06RD

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00917 QueE, 1 hit
InterProiView protein in InterPro
IPR024924 7-CO-7-deazaguanine_synth-like
IPR013785 Aldolase_TIM
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF000370 QueE, 1 hit
SFLDiSFLDS00029 Radical_SAM, 1 hit

Sequencei

Sequence statusi: Complete.

Q2FS67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLNCDTKTA GEISSSIPSG SGSHQPAAQS SGMKIAEIFT SLQGEGLTSG
60 70 80 90 100
YPTIFIRLAG CNLSCSYCDT PASRQGGMDM NVSEVVAGAL LQKPHYVCIT
110 120 130 140 150
GGEPLLQKDE VAELARQLIK AGKMVSIETN GTVPFDDLPS DISICMDVKC
160 170 180 190 200
PSSGEFSNIN LLSDLKSTDS VKFVVGTDDD LQYAEKVIMS HPTKAEIFIS
210 220 230
PIYGTDYQRI ASYILSRNLP ARMQLQLHKF IGLP
Length:234
Mass (Da):25,117
Last modified:March 21, 2006 - v1
Checksum:iB2D68E9894BC36C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA Translation: ABD42524.1

Genome annotation databases

EnsemblBacteriaiABD42524; ABD42524; Mhun_2832
KEGGimhu:Mhun_2832

Similar proteinsi

Entry informationi

Entry nameiQUEE_METHJ
AccessioniPrimary (citable) accession number: Q2FS67
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: March 21, 2006
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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