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Protein

7-carboxy-7-deazaguanine synthase

Gene

queE

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.UniRule annotation

Catalytic activityi

6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH3.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation
  • Mg2+UniRule annotation

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571SubstrateUniRule annotation
Metal bindingi61 – 611Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi65 – 651Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi68 – 681Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi70 – 701MagnesiumUniRule annotation
Binding sitei100 – 1001SubstrateUniRule annotation
Binding sitei102 – 1021S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei234 – 2341Substrate; via carboxylateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMHUN323259:GH0L-2873-MONOMER.
UniPathwayiUPA00391.

Names & Taxonomyi

Protein namesi
Recommended name:
7-carboxy-7-deazaguanine synthaseUniRule annotation (EC:4.3.99.3UniRule annotation)
Short name:
CDG synthaseUniRule annotation
Alternative name(s):
Archaeosine biosynthesis protein QueEUniRule annotation
Gene namesi
Name:queEUniRule annotation
Ordered Locus Names:Mhun_2832
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
Proteomesi
  • UP000001941 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2342347-carboxy-7-deazaguanine synthasePRO_0000416220Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_2832.

Structurei

3D structure databases

ProteinModelPortaliQ2FS67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 443Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02173. Archaea.
COG0602. LUCA.
HOGENOMiHOG000266145.
KOiK10026.
OMAiCDTEYAF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00917. QueE.
InterProiIPR024924. 7-CO-7-deazaguanine_synth-like.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000370. QueE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2FS67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLNCDTKTA GEISSSIPSG SGSHQPAAQS SGMKIAEIFT SLQGEGLTSG
60 70 80 90 100
YPTIFIRLAG CNLSCSYCDT PASRQGGMDM NVSEVVAGAL LQKPHYVCIT
110 120 130 140 150
GGEPLLQKDE VAELARQLIK AGKMVSIETN GTVPFDDLPS DISICMDVKC
160 170 180 190 200
PSSGEFSNIN LLSDLKSTDS VKFVVGTDDD LQYAEKVIMS HPTKAEIFIS
210 220 230
PIYGTDYQRI ASYILSRNLP ARMQLQLHKF IGLP
Length:234
Mass (Da):25,117
Last modified:March 21, 2006 - v1
Checksum:iB2D68E9894BC36C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA. Translation: ABD42524.1.

Genome annotation databases

EnsemblBacteriaiABD42524; ABD42524; Mhun_2832.
KEGGimhu:Mhun_2832.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA. Translation: ABD42524.1.

3D structure databases

ProteinModelPortaliQ2FS67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323259.Mhun_2832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD42524; ABD42524; Mhun_2832.
KEGGimhu:Mhun_2832.

Phylogenomic databases

eggNOGiarCOG02173. Archaea.
COG0602. LUCA.
HOGENOMiHOG000266145.
KOiK10026.
OMAiCDTEYAF.

Enzyme and pathway databases

UniPathwayiUPA00391.
BioCyciMHUN323259:GH0L-2873-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00917. QueE.
InterProiIPR024924. 7-CO-7-deazaguanine_synth-like.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000370. QueE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

Entry informationi

Entry nameiQUEE_METHJ
AccessioniPrimary (citable) accession number: Q2FS67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: March 21, 2006
Last modified: February 17, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.