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Q2FS43 (KAE1B_METHJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. tRNA N6-adenosine threonylcarbamoyltransferase
    EC=2.6.99.4
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name=t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
  2. Serine/threonine-protein kinase Bud32
    EC=2.7.11.1
Gene names
Ordered Locus Names:Mhun_2868
OrganismMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1) [Reference proteome] [HAMAP]
Taxonomic identifier323259 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

L-threonylcarbamoyladenylate + adenine37 in tRNA = AMP + N(6)-L-threonylcarbamoyladenine37 in tRNA. HAMAP-Rule MF_01447

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01447

Subunit structure

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / TsaD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 520520Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000303658

Regions

Domain327 – 520194Protein kinase
Nucleotide binding333 – 3419ATP By similarity
Region1 – 318318Kae1 HAMAP-Rule MF_01447
Region126 – 1305Threonylcarbamoyl-AMP binding By similarity

Sites

Active site4371Proton acceptor; for kinase activity By similarity
Metal binding1051Iron By similarity
Metal binding1091Iron By similarity
Metal binding1261Iron By similarity
Metal binding2791Iron By similarity
Binding site1581Threonylcarbamoyl-AMP By similarity
Binding site1711Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding site1751Threonylcarbamoyl-AMP By similarity
Binding site2511Threonylcarbamoyl-AMP By similarity
Binding site3501ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2FS43 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 484EF8CF82FA8426

FASTA52056,757
        10         20         30         40         50         60 
MKIGPVLGIE GTAWNLSAAL FDDDLIKLVS HPYKPVQGGI HPREAAQHHA SVITSVIEEV 

        70         80         90        100        110        120 
LKGNPTPVAV AFSQGPGLGP CLRIVGTAAR ALALSFDVPL IGVNHCVAHV EIGRFASGFD 

       130        140        150        160        170        180 
DPVVLYASGA NTQVLGYLQG RYRIFGETLD IGIGNAIDKF ARSKGLPHPG GPEIERIAKN 

       190        200        210        220        230        240 
GSYIPLPYTV KGMDLAFSGL VSAAKDASAP LEDVCYSLQE TAFAMCTEVT ERALSQTGKE 

       250        260        270        280        290        300 
QLILVGGVGM NKRLQEMLSC MCEDRDAAFS VPNPQYLGDN GAMIAYTGRV MLESGSVLPV 

       310        320        330        340        350        360 
EESRVNPSYR ADQVLVTWRE EPSGSERHPD AYSARGAEAI VRFCDGAASK IRVSKRYRHP 

       370        380        390        400        410        420 
ELDRRLIAER TRAEARLIAE ARKAGVRTPI IREITQDTII MEHIDGVKLK ECLSPELLEE 

       430        440        450        460        470        480 
TGRMVGKLHA AQIVHGDLTT CNFLVHDGKT WLIDFGLAGT SSDIEHRGVD IHVLFQVLES 

       490        500        510        520 
TSKDSDILKE AFIQGYREKM PLADEILNRE HEIELRGRYL 

« Hide

References

[1]"Complete sequence of Methanospirillum hungatei JF-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000254 Genomic DNA. Translation: ABD42560.1.
RefSeqYP_504279.1. NC_007796.1.

3D structure databases

ProteinModelPortalQ2FS43.
SMRQ2FS43. Positions 6-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323259.Mhun_2868.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD42560; ABD42560; Mhun_2868.
GeneID3923825.
KEGGmhu:Mhun_2868.

Phylogenomic databases

eggNOGCOG0533.
HOGENOMHOG000109569.
KOK15904.
OMARDNAGMI.

Enzyme and pathway databases

BioCycMHUN323259:GH0L-2911-MONOMER.

Family and domain databases

HAMAPMF_01447. Kae1_Bud32_arch.
InterProIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. SSF56112. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEPS01016. GLYCOPROTEASE. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAE1B_METHJ
AccessionPrimary (citable) accession number: Q2FS43
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 21, 2006
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families