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Q2FS43

- KAE1B_METHJ

UniProt

Q2FS43 - KAE1B_METHJ

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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

Mhun_2868

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi105 – 1051IronUniRule annotation
Metal bindingi109 – 1091IronUniRule annotation
Metal bindingi126 – 1261IronUniRule annotation
Binding sitei158 – 1581Threonylcarbamoyl-AMPUniRule annotation
Binding sitei171 – 1711Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
Binding sitei175 – 1751Threonylcarbamoyl-AMPUniRule annotation
Binding sitei251 – 2511Threonylcarbamoyl-AMPUniRule annotation
Metal bindingi279 – 2791IronUniRule annotation
Binding sitei350 – 3501ATPUniRule annotation
Active sitei437 – 4371Proton acceptor; for kinase activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi333 – 3419ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMHUN323259:GH0L-2911-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:Mhun_2868
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
ProteomesiUP000001941: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303658Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_2868.

Structurei

3D structure databases

ProteinModelPortaliQ2FS43.
SMRiQ2FS43. Positions 6-319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini327 – 520194Protein kinaseUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 318318Kae1Add
BLAST
Regioni126 – 1305Threonylcarbamoyl-AMP bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
HOGENOMiHOG000109569.
KOiK15904.
OMAiRDNAGMI.

Family and domain databases

HAMAPiMF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2FS43-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIGPVLGIE GTAWNLSAAL FDDDLIKLVS HPYKPVQGGI HPREAAQHHA
60 70 80 90 100
SVITSVIEEV LKGNPTPVAV AFSQGPGLGP CLRIVGTAAR ALALSFDVPL
110 120 130 140 150
IGVNHCVAHV EIGRFASGFD DPVVLYASGA NTQVLGYLQG RYRIFGETLD
160 170 180 190 200
IGIGNAIDKF ARSKGLPHPG GPEIERIAKN GSYIPLPYTV KGMDLAFSGL
210 220 230 240 250
VSAAKDASAP LEDVCYSLQE TAFAMCTEVT ERALSQTGKE QLILVGGVGM
260 270 280 290 300
NKRLQEMLSC MCEDRDAAFS VPNPQYLGDN GAMIAYTGRV MLESGSVLPV
310 320 330 340 350
EESRVNPSYR ADQVLVTWRE EPSGSERHPD AYSARGAEAI VRFCDGAASK
360 370 380 390 400
IRVSKRYRHP ELDRRLIAER TRAEARLIAE ARKAGVRTPI IREITQDTII
410 420 430 440 450
MEHIDGVKLK ECLSPELLEE TGRMVGKLHA AQIVHGDLTT CNFLVHDGKT
460 470 480 490 500
WLIDFGLAGT SSDIEHRGVD IHVLFQVLES TSKDSDILKE AFIQGYREKM
510 520
PLADEILNRE HEIELRGRYL
Length:520
Mass (Da):56,757
Last modified:March 21, 2006 - v1
Checksum:i484EF8CF82FA8426
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000254 Genomic DNA. Translation: ABD42560.1.
RefSeqiWP_011449813.1. NC_007796.1.
YP_504279.1. NC_007796.1.

Genome annotation databases

EnsemblBacteriaiABD42560; ABD42560; Mhun_2868.
GeneIDi3923825.
KEGGimhu:Mhun_2868.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000254 Genomic DNA. Translation: ABD42560.1 .
RefSeqi WP_011449813.1. NC_007796.1.
YP_504279.1. NC_007796.1.

3D structure databases

ProteinModelPortali Q2FS43.
SMRi Q2FS43. Positions 6-319.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 323259.Mhun_2868.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD42560 ; ABD42560 ; Mhun_2868 .
GeneIDi 3923825.
KEGGi mhu:Mhun_2868.

Phylogenomic databases

eggNOGi COG0533.
HOGENOMi HOG000109569.
KOi K15904.
OMAi RDNAGMI.

Enzyme and pathway databases

BioCyci MHUN323259:GH0L-2911-MONOMER.

Family and domain databases

HAMAPi MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

Entry informationi

Entry nameiKAE1B_METHJ
AccessioniPrimary (citable) accession number: Q2FS43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 21, 2006
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3