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Q2FS43

- KAE1B_METHJ

UniProt

Q2FS43 - KAE1B_METHJ

Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

Mhun_2868

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.UniRule annotation
    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi105 – 1051IronUniRule annotation
    Metal bindingi109 – 1091IronUniRule annotation
    Metal bindingi126 – 1261IronUniRule annotation
    Binding sitei158 – 1581Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei171 – 1711Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
    Binding sitei175 – 1751Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei251 – 2511Threonylcarbamoyl-AMPUniRule annotation
    Metal bindingi279 – 2791IronUniRule annotation
    Binding sitei350 – 3501ATPUniRule annotation
    Active sitei437 – 4371Proton acceptor; for kinase activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi333 – 3419ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. metalloendopeptidase activity Source: InterPro
    4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
    5. protein serine/threonine kinase activity Source: UniProtKB-KW
    6. protein tyrosine kinase activity Source: InterPro
    7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Iron, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMHUN323259:GH0L-2911-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
    Including the following 2 domains:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name:
    t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
    Gene namesi
    Ordered Locus Names:Mhun_2868
    OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
    Taxonomic identifieri323259 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
    ProteomesiUP000001941: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 520520Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303658Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

    Protein-protein interaction databases

    STRINGi323259.Mhun_2868.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2FS43.
    SMRiQ2FS43. Positions 6-319.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini327 – 520194Protein kinaseUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 318318Kae1Add
    BLAST
    Regioni126 – 1305Threonylcarbamoyl-AMP bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
    In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
    Contains 1 protein kinase domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    HOGENOMiHOG000109569.
    KOiK15904.
    OMAiRDNAGMI.

    Family and domain databases

    HAMAPiMF_01447. Kae1_Bud32_arch.
    InterProiIPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR017860. Peptidase_M22_CS.
    IPR000719. Prot_kinase_dom.
    IPR018934. RIO-like_kinase.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    PF01163. RIO1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSiPR00789. OSIALOPTASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2FS43-1 [UniParc]FASTAAdd to Basket

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    MKIGPVLGIE GTAWNLSAAL FDDDLIKLVS HPYKPVQGGI HPREAAQHHA    50
    SVITSVIEEV LKGNPTPVAV AFSQGPGLGP CLRIVGTAAR ALALSFDVPL 100
    IGVNHCVAHV EIGRFASGFD DPVVLYASGA NTQVLGYLQG RYRIFGETLD 150
    IGIGNAIDKF ARSKGLPHPG GPEIERIAKN GSYIPLPYTV KGMDLAFSGL 200
    VSAAKDASAP LEDVCYSLQE TAFAMCTEVT ERALSQTGKE QLILVGGVGM 250
    NKRLQEMLSC MCEDRDAAFS VPNPQYLGDN GAMIAYTGRV MLESGSVLPV 300
    EESRVNPSYR ADQVLVTWRE EPSGSERHPD AYSARGAEAI VRFCDGAASK 350
    IRVSKRYRHP ELDRRLIAER TRAEARLIAE ARKAGVRTPI IREITQDTII 400
    MEHIDGVKLK ECLSPELLEE TGRMVGKLHA AQIVHGDLTT CNFLVHDGKT 450
    WLIDFGLAGT SSDIEHRGVD IHVLFQVLES TSKDSDILKE AFIQGYREKM 500
    PLADEILNRE HEIELRGRYL 520
    Length:520
    Mass (Da):56,757
    Last modified:March 21, 2006 - v1
    Checksum:i484EF8CF82FA8426
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000254 Genomic DNA. Translation: ABD42560.1.
    RefSeqiWP_011449813.1. NC_007796.1.
    YP_504279.1. NC_007796.1.

    Genome annotation databases

    EnsemblBacteriaiABD42560; ABD42560; Mhun_2868.
    GeneIDi3923825.
    KEGGimhu:Mhun_2868.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000254 Genomic DNA. Translation: ABD42560.1 .
    RefSeqi WP_011449813.1. NC_007796.1.
    YP_504279.1. NC_007796.1.

    3D structure databases

    ProteinModelPortali Q2FS43.
    SMRi Q2FS43. Positions 6-319.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323259.Mhun_2868.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD42560 ; ABD42560 ; Mhun_2868 .
    GeneIDi 3923825.
    KEGGi mhu:Mhun_2868.

    Phylogenomic databases

    eggNOGi COG0533.
    HOGENOMi HOG000109569.
    KOi K15904.
    OMAi RDNAGMI.

    Enzyme and pathway databases

    BioCyci MHUN323259:GH0L-2911-MONOMER.

    Family and domain databases

    HAMAPi MF_01447. Kae1_Bud32_arch.
    InterProi IPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR017860. Peptidase_M22_CS.
    IPR000719. Prot_kinase_dom.
    IPR018934. RIO-like_kinase.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    PF01163. RIO1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSi PR00789. OSIALOPTASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

    Entry informationi

    Entry nameiKAE1B_METHJ
    AccessioniPrimary (citable) accession number: Q2FS43
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3