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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

Mhun_2868

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105IronUniRule annotation1
Metal bindingi109IronUniRule annotation1
Metal bindingi126IronUniRule annotation1
Binding sitei158Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei171Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation1
Binding sitei175Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei251Threonylcarbamoyl-AMPUniRule annotation1
Metal bindingi279IronUniRule annotation1
Binding sitei350ATPUniRule annotation1
Active sitei437Proton acceptor; for kinase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi333 – 341ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Kinase, Multifunctional enzyme, Serine/threonine-protein kinase, Transferase
Biological processtRNA processing
LigandATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMHUN323259:G1G5Z-3043-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:Mhun_2868
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
Proteomesi
  • UP000001941 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003036581 – 520Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinAdd BLAST520

Proteomic databases

PRIDEiQ2FS43

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_2868

Structurei

3D structure databases

ProteinModelPortaliQ2FS43
SMRiQ2FS43
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini327 – 520Protein kinaseUniRule annotationAdd BLAST194

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 318Kae1Add BLAST318
Regioni126 – 130Threonylcarbamoyl-AMP bindingUniRule annotation5

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183 Archaea
arCOG01185 Archaea
COG0533 LUCA
COG3642 LUCA
HOGENOMiHOG000109569
KOiK15904
OMAiGETMDTG
OrthoDBiPOG093Z05SV

Family and domain databases

HAMAPiMF_01446 Kae1, 1 hit
MF_01447 Kae1_Bud32_arch, 1 hit
InterProiView protein in InterPro
IPR022495 Bud32
IPR000905 Gcp-like_dom
IPR034680 Kae1/OSGEP
IPR017861 KAE1/TsaD
IPR022449 Kae1_arc
IPR011009 Kinase-like_dom_sf
IPR017860 Peptidase_M22_CS
IPR000719 Prot_kinase_dom
IPR009220 tRNA_threonyl_synthase/kinase
IPR008266 Tyr_kinase_AS
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF14 PTHR11735:SF14, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PIRSFiPIRSF036401 Gcp_STYKS, 1 hit
PRINTSiPR00789 OSIALOPTASE
SUPFAMiSSF56112 SSF56112, 1 hit
TIGRFAMsiTIGR03724 arch_bud32, 1 hit
TIGR03722 arch_KAE1, 1 hit
TIGR00329 gcp_kae1, 1 hit
PROSITEiView protein in PROSITE
PS01016 GLYCOPROTEASE, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequencei

Sequence statusi: Complete.

Q2FS43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGPVLGIE GTAWNLSAAL FDDDLIKLVS HPYKPVQGGI HPREAAQHHA
60 70 80 90 100
SVITSVIEEV LKGNPTPVAV AFSQGPGLGP CLRIVGTAAR ALALSFDVPL
110 120 130 140 150
IGVNHCVAHV EIGRFASGFD DPVVLYASGA NTQVLGYLQG RYRIFGETLD
160 170 180 190 200
IGIGNAIDKF ARSKGLPHPG GPEIERIAKN GSYIPLPYTV KGMDLAFSGL
210 220 230 240 250
VSAAKDASAP LEDVCYSLQE TAFAMCTEVT ERALSQTGKE QLILVGGVGM
260 270 280 290 300
NKRLQEMLSC MCEDRDAAFS VPNPQYLGDN GAMIAYTGRV MLESGSVLPV
310 320 330 340 350
EESRVNPSYR ADQVLVTWRE EPSGSERHPD AYSARGAEAI VRFCDGAASK
360 370 380 390 400
IRVSKRYRHP ELDRRLIAER TRAEARLIAE ARKAGVRTPI IREITQDTII
410 420 430 440 450
MEHIDGVKLK ECLSPELLEE TGRMVGKLHA AQIVHGDLTT CNFLVHDGKT
460 470 480 490 500
WLIDFGLAGT SSDIEHRGVD IHVLFQVLES TSKDSDILKE AFIQGYREKM
510 520
PLADEILNRE HEIELRGRYL
Length:520
Mass (Da):56,757
Last modified:March 21, 2006 - v1
Checksum:i484EF8CF82FA8426
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA Translation: ABD42560.1
RefSeqiWP_011449813.1, NC_007796.1

Genome annotation databases

EnsemblBacteriaiABD42560; ABD42560; Mhun_2868
GeneIDi3923825
KEGGimhu:Mhun_2868

Similar proteinsi

Entry informationi

Entry nameiKAE1B_METHJ
AccessioniPrimary (citable) accession number: Q2FS43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 21, 2006
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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