Reviewed, UniProtKB/Swiss-Prot Q2FR24 (MCH_METHJ)

Names and origin

Protein names

Recommended name:
    Methenyltetrahydromethanopterin cyclohydrolase
    EC=3.5.4.27
Alternative name(s):
    Methenyl-H4MPT cyclohydrolase

Gene names

Name:	mch
Ordered Locus Names:	Mhun_0444

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864) [Complete proteome] [HAMAP]

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible interconversion of 5-formyl-H4MPT to methenyl-H4MPT+ By similarity.
Catalytic activity	5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = 5-formyl-5,6,7,8-tetrahydromethanopterin. HAMAP MF_00486
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methenyl-H(4)MPT from CO(2): step 3/3. HAMAP MF_00486
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MCH family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methenyltetrahydromethanopterin cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Methenyltetrahydromethanopterin cyclohydrolase HAMAP MF_00486		PRO_1000014398

Sequences

	Sequence		Length	Mass (Da)
	Q2FR24-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: D43F86E187FB26E9	FASTA	315	33,984
	10 20 30 40 50 60 MISVNELALE IFDNLANYSE DFNVAYHELD NGAKIVDCGV SVPGGYAAGR AFTEICMGGL 70 80 90 100 110 120 GEVNFRMGQI KHVPMPFIEV STDYPAISCL GAQKAGWTVK AGNYFAMGSG PARALALKPK 130 140 150 160 170 180 HTYEVIGYED ESDDAVIALE SDHLPNGEVM EKIAKDCGID VENLCAVVAP TASLVGSIQV 190 200 210 220 230 240 CGRCVETAVY KLNELGFDTR KITAAMGTAP IPPVKADATK AMGTTNDATI YHGQISLTMK 250 260 270 280 290 300 APDIVDFLEK IPSNKSKGYG KPFYEIFKEA NFDFYQIDTS LFSPAEVTIN ELTEGKVYHV 310 GEVNPDVTLK SFGYL

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000254 Genomic DNA. Translation: ABD40206.1.
RefSeq	YP_501925.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3925006.
GenomeReviews	Gene locus Mhun_0444 in contig CP000254_GR.
KEGG	mhu:Mhun_0444.
NMPDR	fig|323259.5.peg.465.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FR24.
OMA	Q2FR24. YFAMGSG.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_0444-MON.
Family and domain databases
HAMAP	MF_00486. [Tree]
InterPro	IPR003209. METHMP_CycHdrlase. [Graphical view]
Pfam	PF02289. MCH. 1 hit. [Graphical view]
ProDom	PD011637. Cyclohydrolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03120. one_C_mch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCH_METHJ

Accession

Primary (citable) accession number: Q2FR24

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)