Reviewed, UniProtKB/Swiss-Prot Q2FQV3 (AROD_METHJ)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate dehydratase
      Short name=3-dehydroquinase
    EC=4.2.1.10
Alternative name(s):
    Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	Mhun_0979

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864) [Complete proteome] [HAMAP]

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 3/7. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000325540
Sites
	Active site	122	1	Proton acceptor By similarity
	Active site	147	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FQV3-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 4BC9505875F479C0	FASTA	256	28,756
	10 20 30 40 50 60 MTSDALKADP NRLIKIIASL SSTRELLSPE VTKADALEIR LDLITEPIDD ALHSLRTSFQ 70 80 90 100 110 120 GPMVLTVRSS DEGGAYAGGS AGLWKKLEPY IGLVDIIDLE IRFKDHAPQV KEHNKAIIAS 130 140 150 160 170 180 CHQNRMPDDD EMQALIEELH SFGDIVKIAV QPQTKEDVIR LLKITAACPY PIIMSVTGTV 190 200 210 220 230 240 YRYARPLLCL FGSLYTYCYI HSETSPGQYS LREMQLLAHL LSPGFVDPWF EGRPVRSGDA 250 SGYYERAEQY RKHLEF

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000254 Genomic DNA. Translation: ABD40729.1.
RefSeq	YP_502448.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3923623.
GenomeReviews	Gene locus Mhun_0979 in contig CP000254_GR.
KEGG	mhu:Mhun_0979.
NMPDR	fig|323259.5.peg.1032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FQV3.
OMA	Q2FQV3. YARDERE.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_0979-MON.
Family and domain databases
HAMAP	MF_00214. [Tree]
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
ProDom	PD005337. DHquinase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_METHJ

Accession

Primary (citable) accession number: Q2FQV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 21, 2006
Last modified:	May 5, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)