Reviewed, UniProtKB/Swiss-Prot Q2FQU2 (CHEB2_METHJ)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 2
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	Mhun_0988

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864) [Complete proteome] [HAMAP]

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Chemotaxis response regulator protein-glutamate methylesterase 2 HAMAP MF_00099		PRO_0000264339
Regions
	Domain	3 – 118	116	Response regulatory
	Domain	158 – 348	191	CheB-type methylesterase
Sites
	Active site	170	1	By similarity
	Active site	197	1	By similarity
	Active site	290	1	By similarity
Amino acid modifications
	Modified residue	54	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FQU2-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 7F4FF7EC18BD6643	FASTA	350	38,184
	10 20 30 40 50 60 MIRVLLVDDS PVTLMVLQSI LEKEPDISVI GQAKNGKEAI ILASRLAPDI ITMDINMPDL 70 80 90 100 110 120 DGFATTRQIM ERTPVPIIIV SGIDNLEEIR ASFRAVEAGA LAVFRKPPAY GDPDYEEAVS 130 140 150 160 170 180 EFVNAIRTYS EVKVIRRRSN HLKVPKPEAS TIQIPFQIHQ DIRVIVIGAS TGGPQVIQEI 190 200 210 220 230 240 ISNLPLGFPL PLVLVQHMSP GFIEGLALWL TESTGFPVSI AREGEVLQPG KLYVAPDGIH 250 260 270 280 290 300 TGVTSDLRFS FSISPPEHNL RPSVSYLFRS AAKNLGSHVL GILLSGMGSD GAEELLQIRQ 310 320 330 340 350 NGGCTIIQDR DSSFVYGMPG AAEMLNAGMF SLPPVEIARF LRSLSERRQL

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000254 Genomic DNA. Translation: ABD40738.1.
RefSeq	YP_502457.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FQU2.
Genome annotation databases
GeneID	3924035.
GenomeReviews	Gene locus Mhun_0988 in contig CP000254_GR.
KEGG	mhu:Mhun_0988.
NMPDR	fig|323259.5.peg.1041.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FQU2.
OMA	EESSAVW.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_0988-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_METHJ

Accession

Primary (citable) accession number: Q2FQU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	March 21, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)