Reviewed, UniProtKB/Swiss-Prot Q2FPM5 (RIFK_METHJ)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	Mhun_1259

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864) [Complete proteome] [HAMAP]

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Riboflavin kinase HAMAP MF_01285		PRO_0000322091
Regions
	Nucleotide binding	102 – 107	6	CDP By similarity
	Nucleotide binding	201 – 204	4	CDP By similarity
	Region	1 – 92	92	H-T-H motif-like HAMAP MF_01285
	Region	93 – 221	129	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	131	1	Magnesium By similarity
	Metal binding	133	1	Magnesium By similarity
	Binding site	188	1	FMN By similarity
	Binding site	196	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FPM5-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 6A015D40E52115D2	FASTA	221	24,699
	10 20 30 40 50 60 MVTPEDLECL KRIALMGGLN TPVFLKTISL GSELGFSPQT ASRRLRSLEQ QGFISRTLGT 70 80 90 100 110 120 DGQQVTITHG GEDALRQEYC DYYRLFGRQE KSLMLNGTVQ SGLGEGAYYM SLQPYADQFQ 130 140 150 160 170 180 RILGFTPFPG TLNIRLLPGA TLQRKRLSSA EWKVVQGFES EGRTFGDVRC LPCFIRDVPC 190 200 210 220 GIIIPGRTHY PEELIEVVSP EGLRKRFSLK DGDEVTIEVT L

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000254 Genomic DNA. Translation: ABD41003.1.
RefSeq	YP_502722.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FPM5.
Genome annotation databases
GeneID	3925214.
GenomeReviews	Gene locus Mhun_1259 in contig CP000254_GR.
KEGG	mhu:Mhun_1259.
NMPDR	fig|323259.5.peg.1327.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FPM5.
OMA	GAYYVRQ.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_1259-MON.
Family and domain databases
HAMAP	MF_01285. Fused. [Tree]
InterPro	IPR000835. HTH_MarR. IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. PF01047. MarR. 1 hit. [Graphical view]
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RIFK_METHJ

Accession

Primary (citable) accession number: Q2FPM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 21, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)