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Q2FPJ4

- SYI_METHJ

UniProt

Q2FPJ4 - SYI_METHJ

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei594 – 5941ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMHUN323259:GH0L-617-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Mhun_0606
    OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
    Taxonomic identifieri323259 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
    ProteomesiUP000001941: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10621062Isoleucine--tRNA ligasePRO_1000022154Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi323259.Mhun_0606.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2FPJ4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 5711"HIGH" regionAdd
    BLAST
    Motifi591 – 5955"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKPVHWCL.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2FPJ4-1 [UniParc]FASTAAdd to Basket

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    MQEVTSSFTP RVIEASVQKF WTQEDIYARV QEQNRDGKTW FFVDGPPYTT     50
    GHIHLGTAWN KILKDSILRY KRMHGLHVID RAGYDMHGLP IEVRVEHELG 100
    FENKKDIEAF GIGAFIERCK QFALSHKDIM SEQFKSLGVW MNFDDPYQTI 150
    MPEYIEAAWW TLKQADEKGL LDRGHRVVNW CPRCETAIAD SEVEYWDEQD 200
    PSIFVKFPIH GLMNEYLVIW TTTPWTLPAN VAVAVDKDFI YARVEAIKEG 250
    KKEILWIAKD LVEPVLKRGK YQDYSILSEK TGEELAGTTY DSPLADLIPR 300
    QKEIVHTVVT AGFVEMDNTG MVHIAPGHGW DDYLLGVEKG LDVFCPVDGA 350
    GYYTDEGGIY AGQFVRDANE KILSDLGSHL LGRQKITHRY GHCWRCKTPI 400
    IYRATEQWFI SVPKMKEKML SEIKATSWYP DWAGSARFYD FVSDARDWCI 450
    SRQRYWGIPI PVWQCSSCSA HRVFGTVAEL NAAAGSNLTD PHRPYVDEIT 500
    VPCSCGGTMK RVEDIFDVWF DSAMASWATL GFPRNDALFH EMWPADFITE 550
    GQDQTRGWFY SQLGASTIAF NRSPYKSVLM HGFALDADGR KMSKSLGNVV 600
    TPEEVVQKFG VDVLRLYILS SNAPWEDLKF NWDGVSTVNR TMNILWNVYR 650
    FPLPYMILDG FSPAQTSDGK YDDEYIVRSY REMPEIDRWI ISRINTIARS 700
    VSADMDEYQL HRVTRLLMNF ILEDLSRWYV QIVRPRMWLE EDSPDKKFAY 750
    ETITYCLRTL CRLLAPFTPH ITEAMYENLR LPEDPVSVHM LKWPAGDIRL 800
    IDENLERRMD VVRKFDEAVA NARQAGKRKL RWPVQNVIVV TSSESVIEAF 850
    RSMEDLAKDR ANTRNIEVIQ GSWERMRFNA EPVMKKIGPS FGKKGPVVKG 900
    LIEAADGSAL RKQLEESGSV TLSDGSEEFV LTAEHMTFSQ HLPEGIFGAE 950
    MTDASVYVDT TLTEDLEAEG YSREIIRRLQ EMRKQLDLNV EDNIVIDAVI 1000
    EDVHLRELLS ASWLDLIKQE VRGKTLKIHD SVGGRDGSVL FQLDRDWDIE 1050
    GVNVTLGISL AG 1062
    Length:1,062
    Mass (Da):121,693
    Last modified:March 21, 2006 - v1
    Checksum:i55CE0BEDE48AC9EB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000254 Genomic DNA. Translation: ABD40364.1.
    RefSeqiYP_502083.1. NC_007796.1.

    Genome annotation databases

    EnsemblBacteriaiABD40364; ABD40364; Mhun_0606.
    GeneIDi3924432.
    KEGGimhu:Mhun_0606.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000254 Genomic DNA. Translation: ABD40364.1 .
    RefSeqi YP_502083.1. NC_007796.1.

    3D structure databases

    ProteinModelPortali Q2FPJ4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323259.Mhun_0606.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD40364 ; ABD40364 ; Mhun_0606 .
    GeneIDi 3924432.
    KEGGi mhu:Mhun_0606.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KPVHWCL.

    Enzyme and pathway databases

    BioCyci MHUN323259:GH0L-617-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

    Entry informationi

    Entry nameiSYI_METHJ
    AccessioniPrimary (citable) accession number: Q2FPJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3