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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei594ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMHUN323259:G1G5Z-644-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mhun_0606
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
Proteomesi
  • UP000001941 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221541 – 1062Isoleucine--tRNA ligaseAdd BLAST1062

Proteomic databases

PRIDEiQ2FPJ4

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_0606

Structurei

3D structure databases

ProteinModelPortaliQ2FPJ4
SMRiQ2FPJ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionAdd BLAST11
Motifi591 – 595"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q2FPJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEVTSSFTP RVIEASVQKF WTQEDIYARV QEQNRDGKTW FFVDGPPYTT
60 70 80 90 100
GHIHLGTAWN KILKDSILRY KRMHGLHVID RAGYDMHGLP IEVRVEHELG
110 120 130 140 150
FENKKDIEAF GIGAFIERCK QFALSHKDIM SEQFKSLGVW MNFDDPYQTI
160 170 180 190 200
MPEYIEAAWW TLKQADEKGL LDRGHRVVNW CPRCETAIAD SEVEYWDEQD
210 220 230 240 250
PSIFVKFPIH GLMNEYLVIW TTTPWTLPAN VAVAVDKDFI YARVEAIKEG
260 270 280 290 300
KKEILWIAKD LVEPVLKRGK YQDYSILSEK TGEELAGTTY DSPLADLIPR
310 320 330 340 350
QKEIVHTVVT AGFVEMDNTG MVHIAPGHGW DDYLLGVEKG LDVFCPVDGA
360 370 380 390 400
GYYTDEGGIY AGQFVRDANE KILSDLGSHL LGRQKITHRY GHCWRCKTPI
410 420 430 440 450
IYRATEQWFI SVPKMKEKML SEIKATSWYP DWAGSARFYD FVSDARDWCI
460 470 480 490 500
SRQRYWGIPI PVWQCSSCSA HRVFGTVAEL NAAAGSNLTD PHRPYVDEIT
510 520 530 540 550
VPCSCGGTMK RVEDIFDVWF DSAMASWATL GFPRNDALFH EMWPADFITE
560 570 580 590 600
GQDQTRGWFY SQLGASTIAF NRSPYKSVLM HGFALDADGR KMSKSLGNVV
610 620 630 640 650
TPEEVVQKFG VDVLRLYILS SNAPWEDLKF NWDGVSTVNR TMNILWNVYR
660 670 680 690 700
FPLPYMILDG FSPAQTSDGK YDDEYIVRSY REMPEIDRWI ISRINTIARS
710 720 730 740 750
VSADMDEYQL HRVTRLLMNF ILEDLSRWYV QIVRPRMWLE EDSPDKKFAY
760 770 780 790 800
ETITYCLRTL CRLLAPFTPH ITEAMYENLR LPEDPVSVHM LKWPAGDIRL
810 820 830 840 850
IDENLERRMD VVRKFDEAVA NARQAGKRKL RWPVQNVIVV TSSESVIEAF
860 870 880 890 900
RSMEDLAKDR ANTRNIEVIQ GSWERMRFNA EPVMKKIGPS FGKKGPVVKG
910 920 930 940 950
LIEAADGSAL RKQLEESGSV TLSDGSEEFV LTAEHMTFSQ HLPEGIFGAE
960 970 980 990 1000
MTDASVYVDT TLTEDLEAEG YSREIIRRLQ EMRKQLDLNV EDNIVIDAVI
1010 1020 1030 1040 1050
EDVHLRELLS ASWLDLIKQE VRGKTLKIHD SVGGRDGSVL FQLDRDWDIE
1060
GVNVTLGISL AG
Length:1,062
Mass (Da):121,693
Last modified:March 21, 2006 - v1
Checksum:i55CE0BEDE48AC9EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA Translation: ABD40364.1
RefSeqiWP_011447649.1, NC_007796.1

Genome annotation databases

EnsemblBacteriaiABD40364; ABD40364; Mhun_0606
GeneIDi3924432
KEGGimhu:Mhun_0606

Similar proteinsi

Entry informationi

Entry nameiSYI_METHJ
AccessioniPrimary (citable) accession number: Q2FPJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 21, 2006
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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