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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMHUN323259:GH0L-617-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mhun_0606
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
ProteomesiUP000001941 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10621062Isoleucine--tRNA ligasePRO_1000022154Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_0606.

Structurei

3D structure databases

ProteinModelPortaliQ2FPJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiVLGDWDN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2FPJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEVTSSFTP RVIEASVQKF WTQEDIYARV QEQNRDGKTW FFVDGPPYTT
60 70 80 90 100
GHIHLGTAWN KILKDSILRY KRMHGLHVID RAGYDMHGLP IEVRVEHELG
110 120 130 140 150
FENKKDIEAF GIGAFIERCK QFALSHKDIM SEQFKSLGVW MNFDDPYQTI
160 170 180 190 200
MPEYIEAAWW TLKQADEKGL LDRGHRVVNW CPRCETAIAD SEVEYWDEQD
210 220 230 240 250
PSIFVKFPIH GLMNEYLVIW TTTPWTLPAN VAVAVDKDFI YARVEAIKEG
260 270 280 290 300
KKEILWIAKD LVEPVLKRGK YQDYSILSEK TGEELAGTTY DSPLADLIPR
310 320 330 340 350
QKEIVHTVVT AGFVEMDNTG MVHIAPGHGW DDYLLGVEKG LDVFCPVDGA
360 370 380 390 400
GYYTDEGGIY AGQFVRDANE KILSDLGSHL LGRQKITHRY GHCWRCKTPI
410 420 430 440 450
IYRATEQWFI SVPKMKEKML SEIKATSWYP DWAGSARFYD FVSDARDWCI
460 470 480 490 500
SRQRYWGIPI PVWQCSSCSA HRVFGTVAEL NAAAGSNLTD PHRPYVDEIT
510 520 530 540 550
VPCSCGGTMK RVEDIFDVWF DSAMASWATL GFPRNDALFH EMWPADFITE
560 570 580 590 600
GQDQTRGWFY SQLGASTIAF NRSPYKSVLM HGFALDADGR KMSKSLGNVV
610 620 630 640 650
TPEEVVQKFG VDVLRLYILS SNAPWEDLKF NWDGVSTVNR TMNILWNVYR
660 670 680 690 700
FPLPYMILDG FSPAQTSDGK YDDEYIVRSY REMPEIDRWI ISRINTIARS
710 720 730 740 750
VSADMDEYQL HRVTRLLMNF ILEDLSRWYV QIVRPRMWLE EDSPDKKFAY
760 770 780 790 800
ETITYCLRTL CRLLAPFTPH ITEAMYENLR LPEDPVSVHM LKWPAGDIRL
810 820 830 840 850
IDENLERRMD VVRKFDEAVA NARQAGKRKL RWPVQNVIVV TSSESVIEAF
860 870 880 890 900
RSMEDLAKDR ANTRNIEVIQ GSWERMRFNA EPVMKKIGPS FGKKGPVVKG
910 920 930 940 950
LIEAADGSAL RKQLEESGSV TLSDGSEEFV LTAEHMTFSQ HLPEGIFGAE
960 970 980 990 1000
MTDASVYVDT TLTEDLEAEG YSREIIRRLQ EMRKQLDLNV EDNIVIDAVI
1010 1020 1030 1040 1050
EDVHLRELLS ASWLDLIKQE VRGKTLKIHD SVGGRDGSVL FQLDRDWDIE
1060
GVNVTLGISL AG
Length:1,062
Mass (Da):121,693
Last modified:March 21, 2006 - v1
Checksum:i55CE0BEDE48AC9EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA. Translation: ABD40364.1.
RefSeqiWP_011447649.1. NC_007796.1.
YP_502083.1. NC_007796.1.

Genome annotation databases

EnsemblBacteriaiABD40364; ABD40364; Mhun_0606.
GeneIDi3924432.
KEGGimhu:Mhun_0606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA. Translation: ABD40364.1.
RefSeqiWP_011447649.1. NC_007796.1.
YP_502083.1. NC_007796.1.

3D structure databases

ProteinModelPortaliQ2FPJ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323259.Mhun_0606.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD40364; ABD40364; Mhun_0606.
GeneIDi3924432.
KEGGimhu:Mhun_0606.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiVLGDWDN.

Enzyme and pathway databases

BioCyciMHUN323259:GH0L-617-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

Entry informationi

Entry nameiSYI_METHJ
AccessioniPrimary (citable) accession number: Q2FPJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 21, 2006
Last modified: May 27, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.