Q2FPC7 (G1PDH_METHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Short name=G1P dehydrogenase
Short name=G1PDH
EC=1.1.1.261

Alternative name(s):
Enantiomeric glycerophosphate synthase
sn-glycerol-1-phosphate dehydrogenase

Gene names

Name:	egsA
Ordered Locus Names:	Mhun_1136

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864)

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity. HAMAP MF_00497_A
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00497_A
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497_A
Subcellular location	Cytoplasm Potential HAMAP MF_00497_A.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497_A		PRO_0000350651
Regions
	Nucleotide binding	107 – 111	5	NAD By similarity
	Nucleotide binding	129 – 132	4	NAD By similarity
Sites
	Metal binding	181	1	Zinc; catalytic By similarity
	Metal binding	261	1	Zinc; catalytic By similarity
	Metal binding	277	1	Zinc; catalytic By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	138	1	NAD By similarity
	Binding site	181	1	Substrate By similarity
	Binding site	265	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FPC7 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: A6C175F79048EA59	FASTA	359	38,362
	10 20 30 40 50 60 MSTDPVQVLQ PDGFNKSRWT QLPRDVLIGH QAIMQLPDII ADIKPGRSVL LISGGTTREV 70 80 90 100 110 120 AGNTVADILK DQYEVRRFVA GKLDADTLEA CSQASSSADF LIGVGGGRVI DCAKIVSYKQ 130 140 150 160 170 180 GKPFISVPTA ASHDGIISGR ATLPTETGSV SVGAHPPIAV VADTGIISQA PHRLMASGCA 190 200 210 220 230 240 DVISNYTAIL DWELAHRLRG EQISEYAIAL SKMTAEILVK DANLIKPGQE EAAWIVVKAL 250 260 270 280 290 300 VSSGVSMAIA GSSRPASGGE HKFGHALERL MPGAALHGEA CGIGSIMTMY LHGGDWREIR 310 320 330 340 350 SSLARIGAPT TPRELNIPDE VIVEALMKAR DIRPERFTIL DMGLTRESAE HLVQMLYEE

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-1 / DSM 864.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000254 Genomic DNA. Translation: ABD40885.1.
RefSeq	YP_502604.1. NC_007796.1.
3D structure databases
ProteinModelPortal	Q2FPC7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FPC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3922514.
GenomeReviews	Gene locus Mhun_1136 in contig CP000254_GR.
KEGG	mhu:Mhun_1136.
NMPDR	fig|323259.5.peg.1192.
Phylogenomic databases
eggNOG	arNOG04488.
HOGENOM	HBG672951.
OMA	CGVGTIM.
PhylomeDB	Q2FPC7.
ProtClustDB	PRK00843.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_1136-MONOMER.
Family and domain databases
HAMAP	MF_00497_A. G1P_dehydrogenase_A. [Tree]
InterPro	IPR023002. G1P_dehydrogenase_arc. IPR016205. Glycerol_DH. [Graphical view]
KO	K00096.
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_METHJ

Accession

Primary (citable) accession number: Q2FPC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	March 21, 2006
Last modified:	November 16, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program