Reviewed, UniProtKB/Swiss-Prot Q2FNA2 (G3P_METHJ)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	Mhun_2462

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864) [Complete proteome] [HAMAP]

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Glyceraldehyde-3-phosphate dehydrogenase		PRO_0000300974
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	139 – 141	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	194 – 195	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	140	1	Nucleophile By similarity
	Binding site	110	1	NAD; via amide nitrogen By similarity
	Binding site	168	1	NAD By similarity
	Binding site	301	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FNA2-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 3C149595098A350B	FASTA	339	36,818
	10 20 30 40 50 60 MIKVAINGYG TIGKRVADAV SAQKDMEIIG VSKTKPSAEA LIAVQRGYPI YIADMSKKDA 70 80 90 100 110 120 FAKAGIPVAG SVEDMLKKAD IVVDGTPGGV GESNKALYEK AGVKAIWQGG EDHEVAGFSF 130 140 150 160 170 180 NAHANYKDAI GRQFVRVVSC NTTGLCRVIK AVDDAFGVVK VRAVMVRRGA DPHVVKKGPI 190 200 210 220 230 240 DAVVLDPPTI PSHHGPDVNT VLPHIDIVTM AMIVPTTQMH MHAITIELKK EVSRDDVLAV 250 260 270 280 290 300 MRSHNRIGLV QPKTAIKSTA ELKEYVMDMG RPRSDLWENG IFEASVNMVG KELFFFQAIH 310 320 330 QEADVVIENV DAIRAMMGEV RDPETSIRMT NEAMQFTAL

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000254 Genomic DNA. Translation: ABD42162.1.
RefSeq	YP_503881.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3923967.
GenomeReviews	Gene locus Mhun_2462 in contig CP000254_GR.
KEGG	mhu:Mhun_2462.
NMPDR	fig|323259.5.peg.2588.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FNA2.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_2462-MON.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006436. Glyceraldehyde-3-P_DHase_2_arc. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD004105. DapB. 1 hit. PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_METHJ

Accession

Primary (citable) accession number: Q2FNA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 21, 2006
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)