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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase 2 (eno2), Enolase 1 (eno1), Enolase 3 (eno3)
  5. Pyruvate kinase (Mhun_0465)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110NAD; via amide nitrogenUniRule annotation1
Active sitei140NucleophileUniRule annotation1
Binding sitei168NADUniRule annotation1
Binding sitei301NAD; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation (EC:1.2.1.59UniRule annotation)
Short name:
GAPDHUniRule annotation
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gapUniRule annotation
Ordered Locus Names:Mhun_2462
OrganismiMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)
Taxonomic identifieri323259 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum
Proteomesi
  • UP000001941 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003009741 – 339Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST339

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi323259.Mhun_2462.

Structurei

3D structure databases

ProteinModelPortaliQ2FNA2.
SMRiQ2FNA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 141Glyceraldehyde 3-phosphate bindingUniRule annotation3
Regioni194 – 195Glyceraldehyde 3-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
HOGENOMiHOG000223361.
KOiK00150.
OMAiNAIVPNP.
OrthoDBiPOG093Z05Z6.

Family and domain databases

HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiView protein in InterPro
IPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR10836. PTHR10836. 1 hit.
PTHR10836:SF76. PTHR10836:SF76. 1 hit.
PfamiView protein in Pfam
PF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2FNA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVAINGYG TIGKRVADAV SAQKDMEIIG VSKTKPSAEA LIAVQRGYPI
60 70 80 90 100
YIADMSKKDA FAKAGIPVAG SVEDMLKKAD IVVDGTPGGV GESNKALYEK
110 120 130 140 150
AGVKAIWQGG EDHEVAGFSF NAHANYKDAI GRQFVRVVSC NTTGLCRVIK
160 170 180 190 200
AVDDAFGVVK VRAVMVRRGA DPHVVKKGPI DAVVLDPPTI PSHHGPDVNT
210 220 230 240 250
VLPHIDIVTM AMIVPTTQMH MHAITIELKK EVSRDDVLAV MRSHNRIGLV
260 270 280 290 300
QPKTAIKSTA ELKEYVMDMG RPRSDLWENG IFEASVNMVG KELFFFQAIH
310 320 330
QEADVVIENV DAIRAMMGEV RDPETSIRMT NEAMQFTAL
Length:339
Mass (Da):36,818
Last modified:March 21, 2006 - v1
Checksum:i3C149595098A350B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000254 Genomic DNA. Translation: ABD42162.1.
RefSeqiWP_011449420.1. NC_007796.1.

Genome annotation databases

EnsemblBacteriaiABD42162; ABD42162; Mhun_2462.
GeneIDi3923967.
KEGGimhu:Mhun_2462.

Similar proteinsi

Entry informationi

Entry nameiG3P_METHJ
AccessioniPrimary (citable) accession number: Q2FNA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 21, 2006
Last modified: November 22, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families