Q2FN18 (HIS4_METHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
EC=5.3.1.16

Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	Mhun_0921

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864) [Reference proteome] [HAMAP]

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum › Methanospirillum hungatei

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014		PRO_0000290578
Sites
	Active site	8	1	Proton acceptor By similarity
	Active site	129	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FN18 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 097C36232796409D	FASTA	236	24,876
	10 20 30 40 50 60 MIVFPAVDIL GGRCVQLVQG KRETATSYGD PLLCAESWIN QGAEALHIVN LDGAFGSSKL 70 80 90 100 110 120 NAEKITDVII RTGVKTQLGG GIRSLDDARS WLDCGVDRII ISTFAADDPE CLTILSEEYG 130 140 150 160 170 180 SDRIMAGVDA RAGEMVTHGW ERPAGDFLEW ADLFIRKGAG SLLYTNVSVE GLCNGIDPKP 190 200 210 220 230 IRDLLSTVSV PVVVAGGITS PSDIKILKEA DAAGVVLGSA LYSGKITLQE ALEAAG

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-1 / DSM 864.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000254 Genomic DNA. Translation: ABD40671.1.
RefSeq	YP_502390.1. NC_007796.1.
3D structure databases
HSSP	HSSP built from PDB template 1VZW based on UniProtKB P16250.
ProteinModelPortal	Q2FN18.
ModBase	Search...
Protein-protein interaction databases
STRING	323259.Mhun_0921.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD40671; ABD40671; Mhun_0921.
GeneID	3924608.
KEGG	mhu:Mhun_0921.
Phylogenomic databases
eggNOG	COG0106.
HOGENOM	HOG000224614.
KO	K01814.
OMA	DGHCVRL.
ProtClustDB	PRK13585.
Enzyme and pathway databases
BioCyc	MHUN323259:GH0L-984-MONOMER.
UniPathway	UPA00031; UER00009.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01014. HisA.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR023016. Isoase_HisA. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_METHJ

Accession

Primary (citable) accession number: Q2FN18

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	March 21, 2006
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program