Q2FM37 (ILVC_METHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Mhun_1245

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864)

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000252803
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2FM37 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: E0DC84E31EF5EFC0	FASTA	331	36,213
	10 20 30 40 50 60 MLEKYYDKDA DMSAISSKTI AVIGYGSQGR GQALNLKDSG LKVIIGLRPG KSWDLAKSEG 70 80 90 100 110 120 FEVMDVANAA KKADIIQILI PDEQQGAVYK TQIAQGLTKG KTLMFSHGFN IHFGQIVPPA 130 140 150 160 170 180 DVDVIMVAPK GPGHMVRRTY TEGKGVPALI AIHQDVSGNG KKTALAYAKG IGATRAVVFE 190 200 210 220 230 240 TSFREETETD LFGEQAVLCG GMTSLIKAGF ETLVEAGYAP EMAYLEVLHE TKLIVDLIYE 250 260 270 280 290 300 GGFTKMRNSI SNTAQFGDLT RGPRVIGQES YLAMQEILEE IQTGAFAKEW MLENMVNRPV 310 320 330 FNALTRADEE HLIEEVGKEI RATMPQFKDL K

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-1 / DSM 864.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000254 Genomic DNA. Translation: ABD40990.1.
RefSeq	YP_502709.1. NC_007796.1.
3D structure databases
ProteinModelPortal	Q2FM37.
SMR	Q2FM37. Positions 2-326.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FM37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3924187.
GenomeReviews	Gene locus Mhun_1245 in contig CP000254_GR.
KEGG	mhu:Mhun_1245.
NMPDR	fig|323259.5.peg.1311.
Phylogenomic databases
eggNOG	arNOG04335.
HOGENOM	HBG286525.
OMA	RPGKSYE.
PhylomeDB	Q2FM37.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_1245-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_METHJ

Accession

Primary (citable) accession number: Q2FM37

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	March 21, 2006
Last modified:	November 16, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program