Q2FLY5 (SYP_METHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Mhun_1139

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864)

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Proline--tRNA ligase HAMAP MF_01571		PRO_0000249164

Sequences

	Sequence		Length	Mass (Da)
	Q2FLY5 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: AC42CD04B2857F6E	FASTA	478	55,079
	10 20 30 40 50 60 MEEQEGTLPQ KADFSGWYND ILWRAEIMDV RYPVKGLYVW FPFGFGIRKR VYGILRELLD 70 80 90 100 110 120 RDHEETLFPL LIPEPEFMKE AEHIKGFENE VYWVTHGGTT PLDIKLALRP TSETAIYPMY 130 140 150 160 170 180 ALWVRSHADL PIKVYQIVNT FRYETKHTRP LIRLREITSF KEAHTVHATW EDAERQVEDA 190 200 210 220 230 240 IARYREFYDR LNIPVLISKR PDWDKFPGAD YTIAVDTIMP DGRTLQIGTV HHLGDHFSKT 250 260 270 280 290 300 FSIQYEDTKG QQQYCHQTCY GISERSIAAL ISMHGDDRGL MLPPEVAPVQ IVIVPVIMKK 310 320 330 340 350 360 REEEIMQAAE LLEKELQNAG YRVKIDTRDL RPGAKYYYWE MRGVPLRLEI GPRDLDNGVV 370 380 390 400 410 420 MGVLRSGGKE SLPRADIIAE VNRKMTEFTA ILQERAKTHI KDHLVMVKEE SLVNEMVSKG 430 440 450 460 470 VVAVHWCGNR ECADRLEEIA DASVLGTEVR TDLIDQTPGT CIICKKPESV VTLIGRSY

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-1 / DSM 864.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000254 Genomic DNA. Translation: ABD40888.1.
RefSeq	YP_502607.1. NC_007796.1.
3D structure databases
ProteinModelPortal	Q2FLY5.
SMR	Q2FLY5. Positions 13-478.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FLY5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3922781.
GenomeReviews	Gene locus Mhun_1139 in contig CP000254_GR.
KEGG	mhu:Mhun_1139.
NMPDR	fig|323259.5.peg.1195.
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	Q2FLY5.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_1139-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_METHJ

Accession

Primary (citable) accession number: Q2FLY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program