Q2FLH1 (CAPPA_METHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31

Gene names

Name:	ppcA
Ordered Locus Names:	Mhun_0174

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864) [Reference proteome] [HAMAP]

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum › Methanospirillum hungatei

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_01904
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_01904
Cofactor	Magnesium By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the PEPCase type 2 family.
Sequence caution	The sequence ABD39949.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Carbon dioxide fixation
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: HAMAP oxaloacetate metabolic process Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Phosphoenolpyruvate carboxylase HAMAP-Rule MF_01904		PRO_0000309605

Sequences

	Sequence		Length	Mass (Da)
	Q2FLH1 [UniParc]. Last modified May 3, 2011. Version 2. Checksum: 9EAF45C89E40AC3B	FASTA	484	55,411
	10 20 30 40 50 60 MKIPRTMSTQ HPDNVHTPFF TENIELTGED EVKEAYYVYS HLGCTEQMWD CEGKEVDNYV 70 80 90 100 110 120 VKKLLSRYGD YFKEHHLGRD LFLTLRVPNP EIERTEAKIL LETLGSIPRS YDVAHHFYND 130 140 150 160 170 180 TYAPIFEIIL PMTTSFASID NIYQYYCDFV IGQQYKRLGG RDLTIAEWIG PFHPDKIRVI 190 200 210 220 230 240 PLFEDKDGML AADTILRRYF QDKDLDYQRV FLARSDPAVN YGQIGAVLLN KIALWRLHLL 250 260 270 280 290 300 SEDTGIPVYP IIGAGSAPFR GNLRPDTVRR VTDEYAGAYT FTIQSAFKYD TNLEEAVSAI 310 320 330 340 350 360 RYLEEREITP AREIDDTYAI SLIERYAAGY QKQIRSLAPL INRVAAYIPS RRKRKLHVGL 370 380 390 400 410 420 FGYSRNMGGV SLPRAITVTA ALYSIGIPPE VLGLSALTEN DREFVLENYR YVTEDLSDAC 430 440 450 460 470 480 RYLNPDSSFL PDEIKKILPD WIEIDPHSEH RVLSGQIEKA VTACQIDSVQ DMIIRAGAIR KFLG

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-1 / DSM 864.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000254 Genomic DNA. Translation: ABD39949.1. Different initiation.
RefSeq	YP_501668.1. NC_007796.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	323259.Mhun_0174.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD39949; ABD39949; Mhun_0174.
GeneID	3922617.
KEGG	mhu:Mhun_0174.
Phylogenomic databases
eggNOG	COG1892.
HOGENOM	HOG000038601.
KO	K01595.
ProtClustDB	PRK13655.
Enzyme and pathway databases
BioCyc	MHUN323259:GH0L-238-MONOMER.
Family and domain databases
HAMAP	MF_01904. PEPcase_type2.
InterPro	IPR007566. PEP_COase_arc-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PIRSF	PIRSF006677. UCP006677. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR02751. PEPCase_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAPPA_METHJ

Accession

Primary (citable) accession number: Q2FLH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	May 3, 2011
Last modified:	May 1, 2013
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program