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Q2FLH1 (CAPPA_METHJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxylase

Short name=PEPC
Short name=PEPCase
EC=4.1.1.31
Gene names
Name:ppcA
Ordered Locus Names:Mhun_0174
OrganismMethanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1) [Reference proteome] [HAMAP]
Taxonomic identifier323259 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanospirillaceaeMethanospirillum

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_01904

Catalytic activity

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_01904

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01904

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01904

Sequence similarities

Belongs to the PEPCase type 2 family.

Sequence caution

The sequence ABD39949.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processCarbon dioxide fixation
   LigandMagnesium
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbon fixation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

oxaloacetate metabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484Phosphoenolpyruvate carboxylase HAMAP-Rule MF_01904
PRO_0000309605

Sequences

Sequence LengthMass (Da)Tools
Q2FLH1 [UniParc].

Last modified May 3, 2011. Version 2.
Checksum: 9EAF45C89E40AC3B

FASTA48455,411
        10         20         30         40         50         60 
MKIPRTMSTQ HPDNVHTPFF TENIELTGED EVKEAYYVYS HLGCTEQMWD CEGKEVDNYV 

        70         80         90        100        110        120 
VKKLLSRYGD YFKEHHLGRD LFLTLRVPNP EIERTEAKIL LETLGSIPRS YDVAHHFYND 

       130        140        150        160        170        180 
TYAPIFEIIL PMTTSFASID NIYQYYCDFV IGQQYKRLGG RDLTIAEWIG PFHPDKIRVI 

       190        200        210        220        230        240 
PLFEDKDGML AADTILRRYF QDKDLDYQRV FLARSDPAVN YGQIGAVLLN KIALWRLHLL 

       250        260        270        280        290        300 
SEDTGIPVYP IIGAGSAPFR GNLRPDTVRR VTDEYAGAYT FTIQSAFKYD TNLEEAVSAI 

       310        320        330        340        350        360 
RYLEEREITP AREIDDTYAI SLIERYAAGY QKQIRSLAPL INRVAAYIPS RRKRKLHVGL 

       370        380        390        400        410        420 
FGYSRNMGGV SLPRAITVTA ALYSIGIPPE VLGLSALTEN DREFVLENYR YVTEDLSDAC 

       430        440        450        460        470        480 
RYLNPDSSFL PDEIKKILPD WIEIDPHSEH RVLSGQIEKA VTACQIDSVQ DMIIRAGAIR 


KFLG 

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References

[1]"Complete sequence of Methanospirillum hungatei JF-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27890 / DSM 864 / NBRC 100397 / JF-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000254 Genomic DNA. Translation: ABD39949.1. Different initiation.
RefSeqYP_501668.1. NC_007796.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323259.Mhun_0174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD39949; ABD39949; Mhun_0174.
GeneID3922617.
KEGGmhu:Mhun_0174.

Phylogenomic databases

eggNOGCOG1892.
HOGENOMHOG000038601.
KOK01595.

Enzyme and pathway databases

BioCycMHUN323259:GH0L-182-MONOMER.

Family and domain databases

HAMAPMF_01904. PEPcase_type2.
InterProIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamPF14010. PEPcase_2. 1 hit.
[Graphical view]
PIRSFPIRSF006677. UCP006677. 1 hit.
SUPFAMSSF51621. SSF51621. 1 hit.
TIGRFAMsTIGR02751. PEPCase_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCAPPA_METHJ
AccessionPrimary (citable) accession number: Q2FLH1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: May 3, 2011
Last modified: May 14, 2014
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families