Reviewed, UniProtKB/Swiss-Prot Q2FLH1 (CAPPA_METHJ)

Names and origin

Protein names

Recommended name:
    Phosphoenolpyruvate carboxylase
      Short name=PEPCase
      Short name=PEPC
    EC=4.1.1.31

Gene names

Name:	ppcA
Ordered Locus Names:	Mhun_0174

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864) [Complete proteome] [HAMAP]

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity.
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + CO2. HAMAP MF_01904
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_01904
Sequence similarities	Belongs to the PEPCase type 2 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Tricarboxylic acid cycle
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbon utilization by fixation of carbon dioxide Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Phosphoenolpyruvate carboxylase HAMAP MF_01904		PRO_0000309605

Sequences

	Sequence		Length	Mass (Da)
	Q2FLH1-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 7A7F85B721F42C15	FASTA	492	56,341
	10 20 30 40 50 60 MNEQYGKPLK IPRTMSTQHP DNVHTPFFTE NIELTGEDEV KEAYYVYSHL GCTEQMWDCE 70 80 90 100 110 120 GKEVDNYVVK KLLSRYGDYF KEHHLGRDLF LTLRVPNPEI ERTEAKILLE TLGSIPRSYD 130 140 150 160 170 180 VAHHFYNDTY APIFEIILPM TTSFASIDNI YQYYCDFVIG QQYKRLGGRD LTIAEWIGPF 190 200 210 220 230 240 HPDKIRVIPL FEDKDGMLAA DTILRRYFQD KDLDYQRVFL ARSDPAVNYG QIGAVLLNKI 250 260 270 280 290 300 ALWRLHLLSE DTGIPVYPII GAGSAPFRGN LRPDTVRRVT DEYAGAYTFT IQSAFKYDTN 310 320 330 340 350 360 LEEAVSAIRY LEEREITPAR EIDDTYAISL IERYAAGYQK QIRSLAPLIN RVAAYIPSRR 370 380 390 400 410 420 KRKLHVGLFG YSRNMGGVSL PRAITVTAAL YSIGIPPEVL GLSALTENDR EFVLENYRYV 430 440 450 460 470 480 TEDLSDACRY LNPDSSFLPD EIKKILPDWI EIDPHSEHRV LSGQIEKAVT ACQIDSVQDM 490 IIRAGAIRKF LG

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000254 Genomic DNA. Translation: ABD39949.1.
RefSeq	YP_501668.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3922617.
GenomeReviews	Gene locus Mhun_0174 in contig CP000254_GR.
KEGG	mhu:Mhun_0174.
NMPDR	fig|323259.5.peg.181.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FLH1.
OMA	Q2FLH1. RSDPAMN.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_0174-MON.
Family and domain databases
HAMAP	MF_01904. [Tree]
InterPro	IPR007566. PEnol-Pyrv_COase_arc. [Graphical view]
Pfam	PF04477. DUF557. 1 hit. [Graphical view]
PIRSF	PIRSF006677. UCP006677. 1 hit.
TIGRFAMs	TIGR02751. PEPCase_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAPPA_METHJ

Accession

Primary (citable) accession number: Q2FLH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)