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Q2FLH1 (CAPPA_METHJ) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31
Gene names
Name:ppcA
Ordered Locus Names:Mhun_0174
OrganismMethanospirillum hungatei (strain JF-1 / DSM 864)
Taxonomic identifier323259 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP MF_01904

Catalytic activity

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP MF_01904

Cofactor

Magnesium By similarity. HAMAP MF_01904

Subunit structure

Homotetramer By similarity. HAMAP MF_01904

Sequence similarities

Belongs to the PEPCase type 2 family.

Sequence caution

The sequence ABD39949.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processCarbon dioxide fixation
   LigandMagnesium
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processcarbon fixation

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: InterPro

   Molecular functionphosphoenolpyruvate carboxylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484Phosphoenolpyruvate carboxylase HAMAP MF_01904
PRO_0000309605

Sequences

Sequence LengthMass (Da)Tools
Q2FLH1 [UniParc].

Last modified May 3, 2011. Version 2.
Checksum: 9EAF45C89E40AC3B

FASTA48455,411
        10         20         30         40         50         60 
MKIPRTMSTQ HPDNVHTPFF TENIELTGED EVKEAYYVYS HLGCTEQMWD CEGKEVDNYV 

        70         80         90        100        110        120 
VKKLLSRYGD YFKEHHLGRD LFLTLRVPNP EIERTEAKIL LETLGSIPRS YDVAHHFYND 

       130        140        150        160        170        180 
TYAPIFEIIL PMTTSFASID NIYQYYCDFV IGQQYKRLGG RDLTIAEWIG PFHPDKIRVI 

       190        200        210        220        230        240 
PLFEDKDGML AADTILRRYF QDKDLDYQRV FLARSDPAVN YGQIGAVLLN KIALWRLHLL 

       250        260        270        280        290        300 
SEDTGIPVYP IIGAGSAPFR GNLRPDTVRR VTDEYAGAYT FTIQSAFKYD TNLEEAVSAI 

       310        320        330        340        350        360 
RYLEEREITP AREIDDTYAI SLIERYAAGY QKQIRSLAPL INRVAAYIPS RRKRKLHVGL 

       370        380        390        400        410        420 
FGYSRNMGGV SLPRAITVTA ALYSIGIPPE VLGLSALTEN DREFVLENYR YVTEDLSDAC 

       430        440        450        460        470        480 
RYLNPDSSFL PDEIKKILPD WIEIDPHSEH RVLSGQIEKA VTACQIDSVQ DMIIRAGAIR 


KFLG

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References

[1]"Complete sequence of Methanospirillum hungatei JF-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JF-1 / DSM 864.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000254 Genomic DNA. Translation: ABD39949.1. Different initiation.
RefSeqYP_501668.1. NC_007796.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ2FLH1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3922617.
GenomeReviewsGene locus Mhun_0174 in contig CP000254_GR.
KEGGmhu:Mhun_0174.
NMPDRfig|323259.5.peg.181.

Phylogenomic databases

eggNOGarNOG04744.
HOGENOMHBG392160.
ProtClustDBPRK13655.

Enzyme and pathway databases

BioCycMHUN323259:MHUN_0174-MONOMER.

Family and domain databases

HAMAPMF_01904. PEPcase_type2.
[Tree]
InterProIPR021135. PEP_COase.
IPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase.
[Graphical view]
KOK01595.
PfamPF00311. PEPcase. 1 hit.
[Graphical view]
PIRSFPIRSF006677. UCP006677. 1 hit.
SUPFAMSSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMsTIGR02751. PEPCase_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCAPPA_METHJ
AccessionPrimary (citable) accession number: Q2FLH1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: May 3, 2011
Last modified: November 16, 2011
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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