Q2FL30 (GUAAA_METHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	Mhun_1588

Organism

Methanospirillum hungatei (strain JF-1 / DSM 864)

Taxonomic identifier

323259 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanospirillaceae › Methanospirillum

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000294268
Regions
	Domain	3 – 188	186	Glutamine amidotransferase type-1
Sites
	Active site	75	1	Nucleophile By similarity
	Active site	162	1	By similarity
	Active site	164	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FL30 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: EDA84824871ADEF0	FASTA	188	20,428
	10 20 30 40 50 60 MLPLYVVNNY GQFNHLILRA LRDLDIDAKL IPNTTPVSEV REGCQGIILG GGPDISRAGL 70 80 90 100 110 120 SHEYVRLGKP VLGICLGLHV IAQEFGGTVQ SGQKGGYGAV EVTITDHDGI LQGYPQTMQV 130 140 150 160 170 180 WASHADEVVT LPGDFDRLAT SSICGNEAIA HKHLPIFGIQ WHPEVSHTFE GHRVFENFFS ICTGQNKG

References

[1]

"Complete sequence of Methanospirillum hungatei JF-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Ivanova N., McInerney M.J., Brockman F., Culley D., Ferry J.G., Gunsalus R.P., Morrison M., Plugge C., Scholten J., Stams A.J.M., Boone D.R., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-1 / DSM 864.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000254 Genomic DNA. Translation: ABD41319.1.
RefSeq	YP_503038.1. NC_007796.1.
3D structure databases
ProteinModelPortal	Q2FL30.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FL30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3923844.
GenomeReviews	Gene locus Mhun_1588 in contig CP000254_GR.
KEGG	mhu:Mhun_1588.
NMPDR	fig|323259.5.peg.1684.
Phylogenomic databases
eggNOG	arNOG05587.
HOGENOM	HBG292341.
OMA	GQYVHRI.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	MHUN323259:MHUN_1588-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_METHJ

Accession

Primary (citable) accession number: Q2FL30

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program