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Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Staphylococcus aureus (strain USA300)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei84Confers resistance to the antibiotic ciprofloxacin1
Active sitei123O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
Biological processAntibiotic resistance
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrAUniRule annotation
Ordered Locus Names:SAUSA300_0006
OrganismiStaphylococcus aureus (strain USA300)
Taxonomic identifieri367830 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001939 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002326981 – 887DNA gyrase subunit AAdd BLAST887

Proteomic databases

PRIDEiQ2FKQ0.

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2FKQ0.
SMRiQ2FKQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi528 – 534GyrA-boxUniRule annotation7

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000076278.
KOiK02469.
OMAiTHHWLLF.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.90.199.10. 1 hit.
HAMAPiMF_01897. GyrA. 1 hit.
InterProiView protein in InterPro
IPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_rep.
IPR035516. Gyrase/topoIV_suA_C.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
PfamiView protein in Pfam
PF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
SMARTiView protein in SMART
SM00434. TOP4c. 1 hit.
SUPFAMiSSF101904. SSF101904. 1 hit.
SSF56719. SSF56719. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2FKQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELPQSRIN ERNITSEMRE SFLDYAMSVI VARALPDVRD GLKPVHRRIL
60 70 80 90 100
YGLNEQGMTP DKSYKKSARI VGDVMGKYHP HGDLSIYEAM VRMAQDFSYR
110 120 130 140 150
YPLVDGQGNF GSMDGDGAAA MRYTEARMTK ITLELLRDIN KDTIDFIDNY
160 170 180 190 200
DGNEREPSVL PARFPNLLAN GASGIAVGMA TNIPPHNLTE LINGVLSLSK
210 220 230 240 250
NPDISIAELM EDIEGPDFPT AGLILGKSGI RRAYETGRGS IQMRSRAVIE
260 270 280 290 300
ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT
310 320 330 340 350
GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE
360 370 380 390 400
ALVHYLEHQK TVVRRRTQYN LRKAKDRAHI LEGLRIALDH IDEIISTIRE
410 420 430 440 450
SDTDKVAMES LQQRFKLSEK QAQAILDMRL RRLTGLERDK IEAEYNELLN
460 470 480 490 500
YISELEAILA DEEVLLQLVR DELTEIRDRF GDDRRTEIQL GGFENLEDED
510 520 530 540 550
LIPEEQIVIT LSHNNYIKRL PVSTYRAQNR GGRGVQGMNT LEEDFVSQLV
560 570 580 590 600
TLSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPVVNA IELENDEVIS
610 620 630 640 650
TMIAVKDLES EDNFLVFATK RGVVKRSALS NFSRINRNGK IAISFREDDE
660 670 680 690 700
LIAVRLTSGQ EDILIGTSHA SLIRFPESTL RPLGRTATGV KGITLREGDE
710 720 730 740 750
VVGLDVAHAN SVDEVLVVTE NGYGKRTPVN DYRLSNRGGK GIKTATITER
760 770 780 790 800
NGNVVCITTV TGEEDLMIVT NAGVIIRLDV ADISQNGRAA QGVRLIRLGD
810 820 830 840 850
DQFVSTVAKV KEDAEDETNE DEQSTSTVSE DGTEQQREAV VNDETPGNAI
860 870 880
HTEVIDSEEN DEDGRIEVRQ DFMDRVEEDI QQSSDEE
Length:887
Mass (Da):99,376
Last modified:March 21, 2006 - v1
Checksum:iC59597B724F9AE1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000255 Genomic DNA. Translation: ABD20924.1.

Genome annotation databases

EnsemblBacteriaiABD20924; ABD20924; SAUSA300_0006.
KEGGisaa:SAUSA300_0006.

Similar proteinsi

Entry informationi

Entry nameiGYRA_STAA3
AccessioniPrimary (citable) accession number: Q2FKQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: March 21, 2006
Last modified: August 30, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families