DNA gyrase subunit A - Staphylococcus aureus (strain USA300)

Contribute

Send feedback

Read comments (?) or add your own

Q2FKQ0 (GYRA_STAA3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 51. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	SAUSA300_0006

Organism

Staphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]

Taxonomic identifier

367830 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus ›

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 887

887

DNA gyrase subunit A HAMAP-Rule MF_01897

PRO_0000232698

Sites

Active site

123

O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Site

Confers resistance to the antibiotic ciprofloxacin

Sequences

Sequence

Length

Mass (Da)

Tools

Q2FKQ0 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: C59597B724F9AE1B
Show »

FASTA

887

99,376

        10         20         30         40         50         60 
MAELPQSRIN ERNITSEMRE SFLDYAMSVI VARALPDVRD GLKPVHRRIL YGLNEQGMTP 

        70         80         90        100        110        120 
DKSYKKSARI VGDVMGKYHP HGDLSIYEAM VRMAQDFSYR YPLVDGQGNF GSMDGDGAAA 

       130        140        150        160        170        180 
MRYTEARMTK ITLELLRDIN KDTIDFIDNY DGNEREPSVL PARFPNLLAN GASGIAVGMA 

       190        200        210        220        230        240 
TNIPPHNLTE LINGVLSLSK NPDISIAELM EDIEGPDFPT AGLILGKSGI RRAYETGRGS 

       250        260        270        280        290        300 
IQMRSRAVIE ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT 

       310        320        330        340        350        360 
GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE ALVHYLEHQK 

       370        380        390        400        410        420 
TVVRRRTQYN LRKAKDRAHI LEGLRIALDH IDEIISTIRE SDTDKVAMES LQQRFKLSEK 

       430        440        450        460        470        480 
QAQAILDMRL RRLTGLERDK IEAEYNELLN YISELEAILA DEEVLLQLVR DELTEIRDRF 

       490        500        510        520        530        540 
GDDRRTEIQL GGFENLEDED LIPEEQIVIT LSHNNYIKRL PVSTYRAQNR GGRGVQGMNT 

       550        560        570        580        590        600 
LEEDFVSQLV TLSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPVVNA IELENDEVIS 

       610        620        630        640        650        660 
TMIAVKDLES EDNFLVFATK RGVVKRSALS NFSRINRNGK IAISFREDDE LIAVRLTSGQ 

       670        680        690        700        710        720 
EDILIGTSHA SLIRFPESTL RPLGRTATGV KGITLREGDE VVGLDVAHAN SVDEVLVVTE 

       730        740        750        760        770        780 
NGYGKRTPVN DYRLSNRGGK GIKTATITER NGNVVCITTV TGEEDLMIVT NAGVIIRLDV 

       790        800        810        820        830        840 
ADISQNGRAA QGVRLIRLGD DQFVSTVAKV KEDAEDETNE DEQSTSTVSE DGTEQQREAV 

       850        860        870        880 
VNDETPGNAI HTEVIDSEEN DEDGRIEVRQ DFMDRVEEDI QQSSDEE

« Hide

References

[1]

"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus."
Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F.
Lancet 367:731-739(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ANTIBIOTIC RESISTANCE.
Strain: USA300.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000255 Genomic DNA. Translation: ABD20924.1.
RefSeq	YP_492728.1. NC_007793.1.
3D structure databases
ProteinModelPortal	Q2FKQ0.
SMR	Q2FKQ0. Positions 31-489.
ModBase	Search...
Protein-protein interaction databases
STRING	451515.SAUSA300_0006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD20924; ABD20924; SAUSA300_0006.
GeneID	3912939.
KEGG	saa:SAUSA300_0006.
PATRIC	19589220. VBIStaAur129981_0053.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0188.
HOGENOM	HOG000076278.
KO	K02469.
OMA	IAQEDVV.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	SAUR451515:GH3C-6-MONOMER.
Family and domain databases
Gene3D	3.30.1360.40. 1 hit. 3.90.199.10. 2 hits.
HAMAP	MF_01897. GyrA.
InterPro	IPR024946. Arg_repress_C-like. IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_STAA3

Accession

Primary (citable) accession number: Q2FKQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	March 21, 2006
Last modified:	May 29, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents