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Q2FKQ0

- GYRA_STAA3

UniProt

Q2FKQ0 - GYRA_STAA3

Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Staphylococcus aureus (strain USA300)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.UniRule annotation

    Catalytic activityi

    ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei84 – 841Confers resistance to the antibiotic ciprofloxacin
    Active sitei123 – 1231O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: UniProtKB-HAMAP
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA-dependent DNA replication Source: UniProtKB-HAMAP
    2. DNA topological change Source: InterPro
    3. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSAUR451515:GH3C-6-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
    Gene namesi
    Name:gyrAUniRule annotation
    Ordered Locus Names:SAUSA300_0006
    OrganismiStaphylococcus aureus (strain USA300)
    Taxonomic identifieri367830 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000001939: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 887887DNA gyrase subunit APRO_0000232698Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis.UniRule annotation

    Protein-protein interaction databases

    STRINGi451515.SAUSA300_0006.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2FKQ0.
    SMRiQ2FKQ0. Positions 31-489.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the topoisomerase GyrA/ParC subunit family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0188.
    HOGENOMiHOG000076278.
    KOiK02469.
    OMAiTTFGFNM.
    OrthoDBiEOG661H5V.

    Family and domain databases

    Gene3Di3.30.1360.40. 1 hit.
    3.90.199.10. 2 hits.
    HAMAPiMF_01897. GyrA.
    InterProiIPR024946. Arg_repress_C-like.
    IPR005743. GyrA.
    IPR006691. GyrA/parC_pinwhl.
    IPR002205. Topo_IIA_A/C.
    IPR013758. Topo_IIA_A/C_ab.
    IPR013760. Topo_IIA_like_dom.
    [Graphical view]
    PfamiPF03989. DNA_gyraseA_C. 6 hits.
    PF00521. DNA_topoisoIV. 1 hit.
    [Graphical view]
    SMARTiSM00434. TOP4c. 1 hit.
    [Graphical view]
    SUPFAMiSSF56719. SSF56719. 1 hit.
    TIGRFAMsiTIGR01063. gyrA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2FKQ0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAELPQSRIN ERNITSEMRE SFLDYAMSVI VARALPDVRD GLKPVHRRIL    50
    YGLNEQGMTP DKSYKKSARI VGDVMGKYHP HGDLSIYEAM VRMAQDFSYR 100
    YPLVDGQGNF GSMDGDGAAA MRYTEARMTK ITLELLRDIN KDTIDFIDNY 150
    DGNEREPSVL PARFPNLLAN GASGIAVGMA TNIPPHNLTE LINGVLSLSK 200
    NPDISIAELM EDIEGPDFPT AGLILGKSGI RRAYETGRGS IQMRSRAVIE 250
    ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT 300
    GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE 350
    ALVHYLEHQK TVVRRRTQYN LRKAKDRAHI LEGLRIALDH IDEIISTIRE 400
    SDTDKVAMES LQQRFKLSEK QAQAILDMRL RRLTGLERDK IEAEYNELLN 450
    YISELEAILA DEEVLLQLVR DELTEIRDRF GDDRRTEIQL GGFENLEDED 500
    LIPEEQIVIT LSHNNYIKRL PVSTYRAQNR GGRGVQGMNT LEEDFVSQLV 550
    TLSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPVVNA IELENDEVIS 600
    TMIAVKDLES EDNFLVFATK RGVVKRSALS NFSRINRNGK IAISFREDDE 650
    LIAVRLTSGQ EDILIGTSHA SLIRFPESTL RPLGRTATGV KGITLREGDE 700
    VVGLDVAHAN SVDEVLVVTE NGYGKRTPVN DYRLSNRGGK GIKTATITER 750
    NGNVVCITTV TGEEDLMIVT NAGVIIRLDV ADISQNGRAA QGVRLIRLGD 800
    DQFVSTVAKV KEDAEDETNE DEQSTSTVSE DGTEQQREAV VNDETPGNAI 850
    HTEVIDSEEN DEDGRIEVRQ DFMDRVEEDI QQSSDEE 887
    Length:887
    Mass (Da):99,376
    Last modified:March 21, 2006 - v1
    Checksum:iC59597B724F9AE1B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000255 Genomic DNA. Translation: ABD20924.1.
    RefSeqiYP_492728.1. NC_007793.1.

    Genome annotation databases

    EnsemblBacteriaiABD20924; ABD20924; SAUSA300_0006.
    GeneIDi3912939.
    KEGGisaa:SAUSA300_0006.
    PATRICi19589220. VBIStaAur129981_0053.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000255 Genomic DNA. Translation: ABD20924.1 .
    RefSeqi YP_492728.1. NC_007793.1.

    3D structure databases

    ProteinModelPortali Q2FKQ0.
    SMRi Q2FKQ0. Positions 31-489.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 451515.SAUSA300_0006.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABD20924 ; ABD20924 ; SAUSA300_0006 .
    GeneIDi 3912939.
    KEGGi saa:SAUSA300_0006.
    PATRICi 19589220. VBIStaAur129981_0053.

    Phylogenomic databases

    eggNOGi COG0188.
    HOGENOMi HOG000076278.
    KOi K02469.
    OMAi TTFGFNM.
    OrthoDBi EOG661H5V.

    Enzyme and pathway databases

    BioCyci SAUR451515:GH3C-6-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1360.40. 1 hit.
    3.90.199.10. 2 hits.
    HAMAPi MF_01897. GyrA.
    InterProi IPR024946. Arg_repress_C-like.
    IPR005743. GyrA.
    IPR006691. GyrA/parC_pinwhl.
    IPR002205. Topo_IIA_A/C.
    IPR013758. Topo_IIA_A/C_ab.
    IPR013760. Topo_IIA_like_dom.
    [Graphical view ]
    Pfami PF03989. DNA_gyraseA_C. 6 hits.
    PF00521. DNA_topoisoIV. 1 hit.
    [Graphical view ]
    SMARTi SM00434. TOP4c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56719. SSF56719. 1 hit.
    TIGRFAMsi TIGR01063. gyrA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus."
      Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F.
      Lancet 367:731-739(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ANTIBIOTIC RESISTANCE.
      Strain: USA300.

    Entry informationi

    Entry nameiGYRA_STAA3
    AccessioniPrimary (citable) accession number: Q2FKQ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3