Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q2FKQ0 (GYRA_STAA3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit A

EC=5.99.1.3
Gene names
Name:gyrA
Ordered Locus Names:SAUSA300_0006
OrganismStaphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]
Taxonomic identifier367830 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length887 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01897.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentCytoplasm
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: InterPro

DNA-dependent DNA replication

Inferred from electronic annotation. Source: UniProtKB-HAMAP

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA topoisomerase type II (ATP-hydrolyzing) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 887887DNA gyrase subunit A HAMAP-Rule MF_01897
PRO_0000232698

Sites

Active site1231O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Site841Confers resistance to the antibiotic ciprofloxacin

Sequences

Sequence LengthMass (Da)Tools
Q2FKQ0 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: C59597B724F9AE1B

FASTA88799,376
        10         20         30         40         50         60 
MAELPQSRIN ERNITSEMRE SFLDYAMSVI VARALPDVRD GLKPVHRRIL YGLNEQGMTP 

        70         80         90        100        110        120 
DKSYKKSARI VGDVMGKYHP HGDLSIYEAM VRMAQDFSYR YPLVDGQGNF GSMDGDGAAA 

       130        140        150        160        170        180 
MRYTEARMTK ITLELLRDIN KDTIDFIDNY DGNEREPSVL PARFPNLLAN GASGIAVGMA 

       190        200        210        220        230        240 
TNIPPHNLTE LINGVLSLSK NPDISIAELM EDIEGPDFPT AGLILGKSGI RRAYETGRGS 

       250        260        270        280        290        300 
IQMRSRAVIE ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT 

       310        320        330        340        350        360 
GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE ALVHYLEHQK 

       370        380        390        400        410        420 
TVVRRRTQYN LRKAKDRAHI LEGLRIALDH IDEIISTIRE SDTDKVAMES LQQRFKLSEK 

       430        440        450        460        470        480 
QAQAILDMRL RRLTGLERDK IEAEYNELLN YISELEAILA DEEVLLQLVR DELTEIRDRF 

       490        500        510        520        530        540 
GDDRRTEIQL GGFENLEDED LIPEEQIVIT LSHNNYIKRL PVSTYRAQNR GGRGVQGMNT 

       550        560        570        580        590        600 
LEEDFVSQLV TLSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPVVNA IELENDEVIS 

       610        620        630        640        650        660 
TMIAVKDLES EDNFLVFATK RGVVKRSALS NFSRINRNGK IAISFREDDE LIAVRLTSGQ 

       670        680        690        700        710        720 
EDILIGTSHA SLIRFPESTL RPLGRTATGV KGITLREGDE VVGLDVAHAN SVDEVLVVTE 

       730        740        750        760        770        780 
NGYGKRTPVN DYRLSNRGGK GIKTATITER NGNVVCITTV TGEEDLMIVT NAGVIIRLDV 

       790        800        810        820        830        840 
ADISQNGRAA QGVRLIRLGD DQFVSTVAKV KEDAEDETNE DEQSTSTVSE DGTEQQREAV 

       850        860        870        880 
VNDETPGNAI HTEVIDSEEN DEDGRIEVRQ DFMDRVEEDI QQSSDEE 

« Hide

References

[1]"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus."
Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F.
Lancet 367:731-739(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ANTIBIOTIC RESISTANCE.
Strain: USA300.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000255 Genomic DNA. Translation: ABD20924.1.
RefSeqYP_492728.1. NC_007793.1.

3D structure databases

ProteinModelPortalQ2FKQ0.
SMRQ2FKQ0. Positions 31-489.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING451515.SAUSA300_0006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD20924; ABD20924; SAUSA300_0006.
GeneID3912939.
KEGGsaa:SAUSA300_0006.
PATRIC19589220. VBIStaAur129981_0053.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0188.
HOGENOMHOG000076278.
KOK02469.
OMATTFGFNM.
OrthoDBEOG661H5V.

Enzyme and pathway databases

BioCycSAUR451515:GH3C-6-MONOMER.

Family and domain databases

Gene3D3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPMF_01897. GyrA.
InterProIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGYRA_STAA3
AccessionPrimary (citable) accession number: Q2FKQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: March 21, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families