Reviewed, UniProtKB/Swiss-Prot Q2FK73 (PTG3C_STAA3)

Names and origin

Protein names

Recommended name:
    PTS system glucose-specific EIICBA component
Alternative name(s):
    EIICBA-Glc
      Short name=EII-Glc
Including the following 3 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component
    3- Recommended name:
            Glucose-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system glucose-specific EIIA component

Gene names

Name:	ptsG
Synonyms:	glcA
Ordered Locus Names:	SAUSA300_0191

Organism

Staphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]

Taxonomic identifier

367830 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	681 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate. Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose transport Inferred from electronic annotation. Source: InterPro phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 681	681	PTS system glucose-specific EIICBA component		PRO_0000351390
Regions
	Transmembrane	16 – 36	21	Potential
	Transmembrane	73 – 93	21	Potential
	Transmembrane	126 – 146	21	Potential
	Transmembrane	170 – 190	21	Potential
	Transmembrane	199 – 219	21	Potential
	Transmembrane	273 – 293	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	328 – 348	21	Potential
	Transmembrane	355 – 375	21	Potential
	Transmembrane	383 – 403	21	Potential
	Domain	3 – 414	412	PTS EIIC type-1
	Domain	425 – 506	82	PTS EIIB type-1
	Domain	551 – 655	105	PTS EIIA type-1
Sites
	Active site	447	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	603	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2FK73-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 7F1F4A2D4FEBD377	FASTA	681	73,925
	10 20 30 40 50 60 MRKKLFGQLQ RIGKALMLPV AILPAAGLLL AIGTAMQGES LQHYLPFIQN GGVQTVAKLM 70 80 90 100 110 120 TGAGGIIFDN LPMIFALGVA IGLAGGDGVA AIAAFVGYII MNKTMGDFLQ VTPKNIGDPA 130 140 150 160 170 180 SGYASILGIP TLQTGVFGGI IIGALAAWCY NKFYNINLPS YLGFFAGKRF VPIMMATTSF 190 200 210 220 230 240 ILAFPMALIW PTIQSGLNAF STGLLDSNTG VAVFLFGFIK RLLIPFGLHH IFHAPFWFEF 250 260 270 280 290 300 GSWKNAAGEI IHGDQRIFIE QIREGAHLTA GKFMQGEFPV MMFGLPAAAL AIYHTAKPEN 310 320 330 340 350 360 KKVVAGLMGS AALTSFLTGI TEPLEFSFLF VAPLLFFIHA VLDGLSFLTL YLLDLHLGYT 370 380 390 400 410 420 FSGGFIDYFL LGILPNKTQW WLVIPVGLVY AVIYYFVFRF LIVKLKYKTP GREDKQSQAA 430 440 450 460 470 480 TASATELPYA VLEAMGGKAN IKHLDACITR LRVEVNDKSK VDVPGLKDLG ASGVLEVGNN 490 500 510 520 530 540 MQAIFGPKSD QIKHEMQQIM NGQVVENPTT MEDDKDETVV VAEDKSATSE LSHIVHAPLT 550 560 570 580 590 600 GEVTPLSEVP DQVFSEKMMG DGIAIKPSQG EVRAPFNGKV QMIFPTKHAI GLVSDSGLEL 610 620 630 640 650 660 LIHIGLDTVK LNGEGFTLHV EEGQEVKQGD LLINFDLDYI RNHAKSDITP IIVTQGNITN 670 680 LDFKQGEHGN ISFGDQLFEA K

References

[1]

"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus." Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F. Lancet 367:731-739(2006) [PubMed: 16517273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000255 Genomic DNA. Translation: ABD20589.1.
RefSeq	YP_492905.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2FK73.
Genome annotation databases
GeneID	3914934.
GenomeReviews	Gene locus SAUSA300_0191 in contig CP000255_GR.
KEGG	saa:SAUSA300_0191.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2FK73.
OMA	RCASPEN.
Family and domain databases
InterPro	IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011299. PTS_IIBC_glc. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
ProDom	PD002243. PTS_EIIA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit. TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTG3C_STAA3

Accession

Primary (citable) accession number: Q2FK73

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	March 21, 2006
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)