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Q2FK43 (PFLA_STAA3) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate formate-lyase-activating enzyme

Short name=PFL-activating enzyme
EC=1.97.1.4
Gene names
Name:pflA
Ordered Locus Names:SAUSA300_0221
OrganismStaphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]
Taxonomic identifier367830 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activity

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the organic radical-activating enzymes family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Pyruvate formate-lyase-activating enzyme
PRO_0000271708

Sites

Metal binding291Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding331Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding361Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2FK43 [UniParc].

Last modified March 21, 2006. Version 1.
Checksum: 892A603E273F6C89

FASTA25128,499
        10         20         30         40         50         60 
MLKGHLHSVE SLGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKISEPSRE VTVDEMVNEI 

        70         80         90        100        110        120 
LPYKPYFDAS GGGVTVSGGE PLLQMPFLEK LFAELKENGV HTCLDTSAGC ANDTKAFQRH 

       130        140        150        160        170        180 
FEELQKHTDL ILLDIKHIDN DKHIRLTGKP NTHILNFARK LSDMKQPVWI RHVLVPGYSD 

       190        200        210        220        230        240 
DKDDLIKLGE FINSLDNVEK FEILPYHQLG VHKWKTLGIA YELEDVEAPD DEAVKAAYRY 

       250 
VNFKGKIPVE L 

« Hide

References

[1]"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus."
Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F.
Lancet 367:731-739(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USA300.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000255 Genomic DNA. Translation: ABD21373.1.
RefSeqYP_492935.1. NC_007793.1.

3D structure databases

ProteinModelPortalQ2FK43.
SMRQ2FK43. Positions 71-207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING451515.SAUSA300_0221.

Proteomic databases

PRIDEQ2FK43.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD21373; ABD21373; SAUSA300_0221.
GeneID3912962.
KEGGsaa:SAUSA300_0221.
PATRIC19589682. VBIStaAur129981_0281.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1180.
HOGENOMHOG000011458.
KOK04069.
OMAGRSNANT.
OrthoDBEOG64FKHC.

Enzyme and pathway databases

BioCycSAUR451515:GH3C-221-MONOMER.

Family and domain databases

InterProIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsTIGR02493. PFLA. 1 hit.
PROSITEPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFLA_STAA3
AccessionPrimary (citable) accession number: Q2FK43
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 21, 2006
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families