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Protein

Pyruvate formate-lyase-activating enzyme

Gene

pflA

Organism
Staphylococcus aureus (strain USA300)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi29Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi33Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi36Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei79S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei207S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Ordered Locus Names:SAUSA300_0221
OrganismiStaphylococcus aureus (strain USA300)
Taxonomic identifieri367830 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001939 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002717081 – 251Pyruvate formate-lyase-activating enzymeAdd BLAST251

Structurei

3D structure databases

ProteinModelPortaliQ2FK43.
SMRiQ2FK43.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 37S-adenosyl-L-methionine bindingBy similarity3
Regioni134 – 136S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000011458.
KOiK04069.
OMAiIGVPNKR.

Family and domain databases

InterProiView protein in InterPro
IPR034457. Organic_radical-activating.
IPR012839. Organic_radical_activase.
IPR012838. PFL1_activating.
IPR034465. Pyruvate_for-lyase_activase.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
PfamiView protein in Pfam
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF000371. PFL_act_enz. 1 hit.
SFLDiSFLDF00278. pyruvate_formate-lyase_activas. 1 hit.
SFLDG01066. organic_radical-activating_enz. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiView protein in PROSITE
PS01087. RADICAL_ACTIVATING. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2FK43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKGHLHSVE SLGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKISEPSRE
60 70 80 90 100
VTVDEMVNEI LPYKPYFDAS GGGVTVSGGE PLLQMPFLEK LFAELKENGV
110 120 130 140 150
HTCLDTSAGC ANDTKAFQRH FEELQKHTDL ILLDIKHIDN DKHIRLTGKP
160 170 180 190 200
NTHILNFARK LSDMKQPVWI RHVLVPGYSD DKDDLIKLGE FINSLDNVEK
210 220 230 240 250
FEILPYHQLG VHKWKTLGIA YELEDVEAPD DEAVKAAYRY VNFKGKIPVE

L
Length:251
Mass (Da):28,499
Last modified:March 21, 2006 - v1
Checksum:i892A603E273F6C89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000255 Genomic DNA. Translation: ABD21373.1.

Genome annotation databases

EnsemblBacteriaiABD21373; ABD21373; SAUSA300_0221.
KEGGisaa:SAUSA300_0221.

Similar proteinsi

Entry informationi

Entry nameiPFLA_STAA3
AccessioniPrimary (citable) accession number: Q2FK43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 21, 2006
Last modified: November 22, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families