Q2FJH7 (SLE1_STAA3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmuramoyl-L-alanine amidase sle1
EC=3.5.1.28

Gene names

Name:	sle1
Synonyms:	aaa
Ordered Locus Names:	SAUSA300_0438

Organism

Staphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]

Taxonomic identifier

367830 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptidoglycan hydrolase involved in the splitting of the septum during cell division By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted By similarity. Cell surface By similarity.
Sequence similarities	Contains 3 LysM repeats. Contains 1 peptidase C51 domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell wall biogenesis/degradation Septation Virulence
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	barrier septum assembly Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 334	309	N-acetylmuramoyl-L-alanine amidase sle1		PRO_0000271076
Regions
	Repeat	29 – 71	43	LysM 1
	Repeat	93 – 135	43	LysM 2
	Repeat	160 – 202	43	LysM 3
	Domain	210 – 334	125	Peptidase C51

Sequences

	Sequence		Length	Mass (Da)
	Q2FJH7 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 4C1E30AD9DE61D36	FASTA	334	35,836
	10 20 30 40 50 60 MQKKVIAAII GTSAISAVAA TQANAATTHT VKPGESVWAI SNKYGISIAK LKSLNNLTSN 70 80 90 100 110 120 LIFPNQVLKV SGSSNSTSNS SRPSTNSGGG SYYTVQAGDS LSLIASKYGT TYQNIMRLNG 130 140 150 160 170 180 LNNFFIYPGQ KLKVSGTASS SNAASNSSRP STNSGGGSYY TVQAGDSLSL IASKYGTTYQ 190 200 210 220 230 240 KIMSLNGLNN FFIYPGQKLK VTGNASTNSG SATTTNRGYN TPVFSHQNLY TWGQCTYHVF 250 260 270 280 290 300 NRRAEIGKGI STYWWNANNW DNAAAADGYT IDNRPTVGSI AQTDVGYYGH VMFVERVNND 310 320 330 GSILVSEMNY SAAPGILTYR TVPAYQVNNY RYIH

References

[1]

"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus." Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F. Lancet 367:731-739(2006) [PubMed: 16517273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USA300.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000255 Genomic DNA. Translation: ABD20550.1.
RefSeq	YP_493151.1. NC_007793.1.
3D structure databases
ProteinModelPortal	Q2FJH7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000038735; EBSTAP00000037443; EBSTAG00000038733.
GeneID	3912915.
GenomeReviews	Gene locus SAUSA300_0438 in contig CP000255_GR.
KEGG	saa:SAUSA300_0438.
PATRIC	19590140. VBIStaAur129981_0510.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1388.
GeneTree	EBGT00050000023682.
HOGENOM	HBG697363.
OMA	LTYRTIP.
ProtClustDB	CLSK884669.
Family and domain databases
InterPro	IPR007921. CHAP. IPR018392. Peptidoglycan-bd_lysin. IPR002482. Peptidoglycan-bd_Lysin_subgr. [Graphical view]
Pfam	PF05257. CHAP. 1 hit. PF01476. LysM. 3 hits. [Graphical view]
SMART	SM00257. LysM. 3 hits. [Graphical view]
PROSITE	PS50911. CHAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SLE1_STAA3

Accession

Primary (citable) accession number: Q2FJH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program