Q2FIE3 (DLTA_STAA3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--poly(phosphoribitol) ligase subunit 1
EC=6.1.1.13

Alternative name(s):
D-alanine-D-alanyl carrier protein ligase
Short name=DCL
D-alanine-activating enzyme
Short name=DAE

Gene names

Name:	dltA
Ordered Locus Names:	SAUSA300_0835

Organism

Staphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]

Taxonomic identifier

367830 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of D-alanyl-lipoteichoic acid (LTA). Catalyzes an ATP-dependent two-step reaction where it forms a high energy D-alanyl AMP intermediate and transfers the alanyl residues from AMP to Dcp By similarity. HAMAP MF_00593
Catalytic activity	ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate). HAMAP MF_00593
Pathway	Cell wall biogenesis; lipoteichoic acid biosynthesis. HAMAP MF_00593
Subcellular location	Cytoplasm Probable HAMAP MF_00593.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	teichoic acid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-poly(phosphoribitol) ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	D-alanine--poly(phosphoribitol) ligase subunit 1 HAMAP MF_00593		PRO_1000025533

Sequences

	Sequence		Length	Mass (Da)
	Q2FIE3 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: FFA535607F1FD328	FASTA	485	54,670
	10 20 30 40 50 60 MTDIINKLQA FADANPQSIA VRHTTDELTY QQLMDESSKL AHRLQGSKKP MILFGHMSPY 70 80 90 100 110 120 MIVGMIGAIK AGCGYVPVDT SIPEDRIKMI INKVQPEFVF NTTDESFESL EGEVFTIEDI 130 140 150 160 170 180 KTSQDPVIFD SQIKDNDTVY TIFTSGSTGE PKGVQIEYAS LVQFTEWMLE LNKSGNEQQW 190 200 210 220 230 240 LNQAPFSFDL SVMAIYPCLA SGGTLNLVDK NMINKPKLLN EMLTATPINI WVSTPSFMEM 250 260 270 280 290 300 CLLLPTLNEE QYGSLNEFFF CGEILPHRAA KALVNRFPSA TIYNTYGPTE ATVAVTSIQI 310 320 330 340 350 360 TQEILDQYPT LPVGVERPGA RLSTTDEGEL VIEGQSVSLG YLKNDQKTAE VFNFDDGIRT 370 380 390 400 410 420 YHTGDKAKFE NGQWFIQGRI DFQIKLNGYR MELEEIETQL RQSEFVKEAI VVPVYKNDKV 430 440 450 460 470 480 IHLIGAIVPT TEVTDNAEMT KNIKNDLKSR LPEYMIPRKF EWMEQLPLTS NGKIDRKKIA EVING

References

[1]

"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus." Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F. Lancet 367:731-739(2006) [PubMed: 16517273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USA300.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000255 Genomic DNA. Translation: ABD22135.1.
RefSeq	YP_493535.1. NC_007793.1.
3D structure databases
ProteinModelPortal	Q2FIE3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000037903; EBSTAP00000036611; EBSTAG00000037901.
GeneID	3913697.
GenomeReviews	Gene locus SAUSA300_0835 in contig CP000255_GR.
KEGG	saa:SAUSA300_0835.
PATRIC	19590989. VBIStaAur129981_0925.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1020.
GeneTree	EBGT00050000024490.
HOGENOM	HBG320748.
OMA	WIYKTEF.
ProtClustDB	PRK04813.
Family and domain databases
HAMAP	MF_00593. DltA. [Tree]
InterPro	IPR010071. AA_adenyl_domain. IPR000873. AMP-dep_Synth/Lig. IPR010072. D_ala_DACP_lig. [Graphical view]
KO	K03367.
PANTHER	PTHR24095:SF34. PTHR24095:SF34. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01733. AA-adenyl-dom. 1 hit. TIGR01734. D-ala-DACP-lig. 1 hit.
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DLTA_STAA3

Accession

Primary (citable) accession number: Q2FIE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program