Q2FI60 (UGTP_STAA3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Processive diacylglycerol glucosyltransferase
EC=2.4.1.-

Alternative name(s):
Beta-gentiobiosyldiacylglycerol synthase
DGlcDAG synthase
Diglucosyldiacylglycerol synthase
Glc2-DAG synthase
Monoglucosyldiacylglycerol synthase
Short name=MGlcDAG synthase
Triglucosyldiacylglycerol synthase
Short name=TGlcDAG synthase
UDP glucosyltransferase
UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferase

Gene names

Name:	ugtP
Ordered Locus Names:	SAUSA300_0918

Organism

Staphylococcus aureus (strain USA300) [Complete proteome] [HAMAP]

Taxonomic identifier

367830 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is a processive glucosyltransferase involved in the synthesis of both bilayer- and non-bilayer-forming membrane glycolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of mono-, di- and triglucosyldiacylglycerol. Diglucosyldiacylglycerol (Glc2-DAG) is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA) By similarity. HAMAP MF_01280
Catalytic activity	UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol. HAMAP MF_01280 UDP-glucose + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol.
Pathway	Glycolipid metabolism; diglucosyl-diacylglycerol biosynthesis. HAMAP MF_01280
Sequence similarities	Belongs to the glycosyltransferase 28 family. UgtP subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolipid biosynthetic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro glucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Processive diacylglycerol glucosyltransferase HAMAP MF_01280		PRO_0000308453

Sequences

	Sequence		Length	Mass (Da)
	Q2FI60 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: A1D6E96C91B7E99D	FASTA	391	44,548
	10 20 30 40 50 60 MVTQNKKILI ITGSFGNGHM QVTQSIVNQL NDMNLDHLSV IEHDLFMEAH PILTSICKKW 70 80 90 100 110 120 YINSFKYFRN MYKGFYYSRP DKLDKCFYKY YGLNKLINLL IKEKPDLILL TFPTPVMSVL 130 140 150 160 170 180 TEQFNINIPV ATVMTDYRLH KNWITPYSTR YYVATKETKQ DFIDVGIDPS TVKVTGIPID 190 200 210 220 230 240 NKFETPINQK QWLIDNNLDP DKQTILMSAG AFGVSKGFDT MITDILAKSA NAQVVMICGK 250 260 270 280 290 300 SKELKRSLTA KFKSNENVLI LGYTKHMNEW MASSQLMITK PGGITITEGF ARCIPMIFLN 310 320 330 340 350 360 PAPGQELENA LYFEEKGFGK IADTPEEAIK IVASLTNGNE QLTNMISTME QDKIKYATQT 370 380 390 ICRDLLDLIG HSSQPQEIYG KVPLYARFFV K

References

[1]

"Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus." Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F. Lancet 367:731-739(2006) [PubMed: 16517273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USA300.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000255 Genomic DNA. Translation: ABD21520.1.
RefSeq	YP_493618.1. NC_007793.1.
3D structure databases
ProteinModelPortal	Q2FI60.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000038294; EBSTAP00000037002; EBSTAG00000038292.
GeneID	3912877.
GenomeReviews	Gene locus SAUSA300_0918 in contig CP000255_GR.
KEGG	saa:SAUSA300_0918.
PATRIC	19591163. VBIStaAur129981_1012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0707.
GeneTree	EBGT00050000024210.
HOGENOM	HBG535586.
OMA	EPAGHIV.
ProtClustDB	PRK13608.
Family and domain databases
HAMAP	MF_01280. Diacylglyc_glucosyltr. [Tree]
InterPro	IPR009695. Diacylglyc_glucosyltr_N. IPR007235. Glyco_trans_28_C. IPR023589. Pro_diacylglycrl_glcsylTrfase. [Graphical view]
KO	K03429.
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF06925. MGDG_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UGTP_STAA3

Accession

Primary (citable) accession number: Q2FI60

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	March 21, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program