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Q2FHI8

- TOP1_STAA3

UniProt

Q2FHI8 - TOP1_STAA3

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Protein
DNA topoisomerase 1
Gene
topA, SAUSA300_1143
Organism
Staphylococcus aureus (strain USA300)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalytic By similarity
Sitei33 – 331Interaction with DNA By similarity
Metal bindingi82 – 821Magnesium 1; catalytic By similarity
Metal bindingi82 – 821Magnesium 2 By similarity
Metal bindingi84 – 841Magnesium 2 By similarity
Sitei139 – 1391Interaction with DNA By similarity
Sitei140 – 1401Interaction with DNA By similarity
Sitei143 – 1431Interaction with DNA By similarity
Sitei148 – 1481Interaction with DNA By similarity
Sitei155 – 1551Interaction with DNA By similarity
Active sitei298 – 2981O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei300 – 3001Interaction with DNA By similarity
Sitei488 – 4881Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri577 – 60327C4-type 1UniRule annotation
Add
BLAST
Zinc fingeri617 – 64529C4-type 2UniRule annotation
Add
BLAST
Zinc fingeri658 – 68124C4-type 3UniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSAUR451515:GH3C-1143-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:SAUSA300_1143
OrganismiStaphylococcus aureus (strain USA300)
Taxonomic identifieri367830 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000001939: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 689689DNA topoisomerase 1UniRule annotation
PRO_0000285943Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi451515.SAUSA300_1143.

Structurei

3D structure databases

ProteinModelPortaliQ2FHI8.
SMRiQ2FHI8. Positions 5-609.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 113111Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1686Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2FHI8-1 [UniParc]FASTAAdd to Basket

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MADNLVIVES PAKAKTIEKY LGKKYKVIAS MGHVRDLPRS QMGVDTEDNY    50
EPKYITIRGK GPVVKELKKH AKKAKNVFLA SDPDREGEAI AWHLSKILEL 100
EDSKENRVVF NEITKDAVKE SFKNPREIEM NLVDAQQARR ILDRLVGYNI 150
SPVLWKKVKK GLSAGRVQSV ALRLVIDREN EIRNFKPEEY WTIEGEFRYK 200
KSKFNAKFLH YKNKPFKLKT KKDVEKITAA LDGDQFEITN VTKKEKTRNP 250
ANPFTTSTLQ QEAARKLNFK ARKTMMVAQQ LYEGIDLKKQ GTIGLITYMR 300
TDSTRISDTA KVEAKQYITD KYGESYTSKR KASGKQGDQD AHEAIRPSST 350
MRTPDDMKSF LTKDQYRLYK LIWERFVASQ MAPAILDTVS LDITQGDIKF 400
RANGQTIKFK GFMTLYVETK DDSDSEKENK LPKLEQGDKV TATQIEPAQH 450
YTQPPPRYTE ARLVKTLEEL KIGRPSTYAP TIDTIQKRNY VKLESKRFVP 500
TELGEIVHEQ VKEYFPEIID VEFTVNMETL LDKIAEGDIT WRKVIDGFFS 550
SFKQDVERAE EEMEKIEIKD EPAGEDCEIC GSPMVIKMGR YGKFMACSNF 600
PDCRNTKAIV KSIGVKCPKC NDGDVVERKS KKNRVFYGCS KYPECDFISW 650
DKPIGRDCPK CNQYLVENKK GKTTQVICSN CDYKEAAQK 689
Length:689
Mass (Da):79,113
Last modified:May 1, 2007 - v2
Checksum:i1F28D3BC10BDADE1
GO

Sequence cautioni

The sequence ABD21813.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000255 Genomic DNA. Translation: ABD21813.1. Different initiation.
RefSeqiYP_493840.1. NC_007793.1.

Genome annotation databases

EnsemblBacteriaiABD21813; ABD21813; SAUSA300_1143.
GeneIDi3915296.
KEGGisaa:SAUSA300_1143.
PATRICi19591653. VBIStaAur129981_1254.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000255 Genomic DNA. Translation: ABD21813.1 . Different initiation.
RefSeqi YP_493840.1. NC_007793.1.

3D structure databases

ProteinModelPortali Q2FHI8.
SMRi Q2FHI8. Positions 5-609.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 451515.SAUSA300_1143.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABD21813 ; ABD21813 ; SAUSA300_1143 .
GeneIDi 3915296.
KEGGi saa:SAUSA300_1143.
PATRICi 19591653. VBIStaAur129981_1254.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci SAUR451515:GH3C-1143-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus."
    Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G., Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F., Perdreau-Remington F.
    Lancet 367:731-739(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: USA300.

Entry informationi

Entry nameiTOP1_STAA3
AccessioniPrimary (citable) accession number: Q2FHI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: September 3, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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