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Protein

Glycerol kinase

Gene

glpK

Organism
Staphylococcus aureus (strain USA300)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12SubstrateUniRule annotation1
Binding sitei16ATPUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei266ATPUniRule annotation1
Binding sitei309ATP; via carbonyl oxygenUniRule annotation1
Binding sitei313ATP; via amide nitrogenUniRule annotation1
Binding sitei328ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 14ATPUniRule annotation3
Nucleotide bindingi410 – 414ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:SAUSA300_1192
OrganismiStaphylococcus aureus (strain USA300)
Taxonomic identifieri367830 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001939 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000207921 – 498Glycerol kinaseAdd BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei230Phosphohistidine; by HPrUniRule annotation1

Post-translational modificationi

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium).UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2FHD9.
SMRiQ2FHD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate bindingUniRule annotation2
Regioni244 – 245Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000222134.
KOiK00864.
OMAiWWLTGGP.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2FHD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKYILSIDQ GTTSSRAILF NQKGEIAGVA QREFKQYFPQ SGWVEHDANE
60 70 80 90 100
IWTSVLAVMT EVINENDVRA DQIAGIGITN QRETTVVWDK HTGRPIYHAI
110 120 130 140 150
VWQSRQTQSI CSELKQQGYE QTFRDKTGLL LDPYFAGTKV KWILDNVEGA
160 170 180 190 200
REKAENGDLL FGTIDTWLVW KLSGKAAHIT DYSNASRTLM FNIHDLEWDD
210 220 230 240 250
ELLELLTVPK NMLPEVKASS EVYGKTIDYH FYGQEVPIAG VAGDQQAALF
260 270 280 290 300
GQACFERGDV KNTYGTGGFM LMNTGDKAVK SESGLLTTIA YGIDGKVNYA
310 320 330 340 350
LEGSIFVSGS AIQWLRDGLR MINSAPQSES YATRVDSTEG VYVVPAFVGL
360 370 380 390 400
GTPYWDSEAR GAIFGLTRGT EKEHFIRATL ESLCYQTRDV MEAMSKDSGI
410 420 430 440 450
DVQSLRVDGG AVKNNFIMQF QADIVNTSVE RPEIQETTAL GAAFLAGLAV
460 470 480 490
GFWESKDDIA KNWKLEEKFD PKMDEGEREK LYRGWKKAVE ATQVFKTE
Length:498
Mass (Da):55,626
Last modified:March 21, 2006 - v1
Checksum:i6B11099ED0AFB561
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000255 Genomic DNA. Translation: ABD20852.1.

Genome annotation databases

EnsemblBacteriaiABD20852; ABD20852; SAUSA300_1192.
KEGGisaa:SAUSA300_1192.
PATRICi19591753. VBIStaAur129981_1304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000255 Genomic DNA. Translation: ABD20852.1.

3D structure databases

ProteinModelPortaliQ2FHD9.
SMRiQ2FHD9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD20852; ABD20852; SAUSA300_1192.
KEGGisaa:SAUSA300_1192.
PATRICi19591753. VBIStaAur129981_1304.

Phylogenomic databases

HOGENOMiHOG000222134.
KOiK00864.
OMAiWWLTGGP.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPK_STAA3
AccessioniPrimary (citable) accession number: Q2FHD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.